Overview of Our Integrated Proteomics and Bioinformatics Services Platform The Systems Proteomics Company 2011 March 25
Overview of Our Integrated Proteomics and Bioinformatics Services Platform The Systems Proteomics Company 2011 March 25.
Mar 26, 2015
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Overview of Our Integrated
Proteomics and Bioinformatics
Services Platform
The Systems Proteomics Company
2011 March 25
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The Kinexus Discovery Platform
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
IMMUNO-HISTOCHEM
GRAPHICSSERVICES
We have created a highly integrated platform of unique proteomics and bioinformatics services to identify disease biomarkers and drug targets and to foster signal transduction research
Our proteomics services are unmatched by any company for convenience, breadth, accuracy, cost and turn-around time
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Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
CELL CULTURE 12 cell lines Kinexus offers cell lysates from 16 of the most commonly studied human tumour cell lines prepared according to client specifications for proteomics analysis
16 cell lines
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Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
CELL CULTURE 12 cell lines16 cell linesANTIBODY
VALIDATION
PROTEINCHARACTERIZATION
>40 monkey, rat+ mouse tissues
ANIMAL TISSUES
Clients can also choose from over 230 pre-made cell and tissue lysates for use in our proteomics services
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Microarray Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
ANTIBODY MICROARRAYS >650 antibodies>800 antibodies
Close up of 1 of 16 grids on a Kinex™ Antibody Microarray - control (blue) and treated (red) have been overlaid and staggered for comparison.
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MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
ANTIBODY MICROARRAYS
REVERSEMICROARRAYS
>650 antibodies>800 antibodies
Microarray Services
>230 lysates
Close up of a section of the Standard Kinex™ Reverse Lysate Microarray. Custom KRLM chips can also be produced for our clients with their own cell/tissue lysates.
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MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
ANTIBODY MICROARRAYS
REVERSEMICROARRAYS
>650 antibodies
PROTEIN KINASE MICROARRAYS
>230 lysates
>800 antibodies
Microarray Services
~200 kinases
Close up of a section of the Kinex™ Protein Kinase Microarray. The binding of an ATP analogue to active protein kinase is shown.
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MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
ANTIBODY MICROARRAYS
REVERSEMICROARRAYS
>650 antibodies
PROTEIN KINASE MICROARRAYS
>230 lysates
>800 antibodies
~200 kinases
PEPTIDEMICROARRAYS
Microarray Services
~450 peptides
Close up of a section of the Kinex™ Kinase Substrate Microarray chip. The binding of a phosphate detection dye to phosphorylated peptides is shown.
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Multi-Immunoblotting Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
PAN-SPECIFIC ABKinases
Phosphatases 2 screens
KPKS-1.2 multi-immunoblot showinghalf of the protein kinases detected with this screen.
Multi-Immunoblotting Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
PAN-SPECIFIC AB
PHOSPHO-SPECIFIC AB
KinasesPhosphatases
Phosphosites
2 screens
4 screens
Overlay of two KPSS multi-immunoblots showing the effects of staurosporin on Jurkat T cell phosphorylation. Red bands correspond to increased phosphorylation and blue bands reveal decreases in phosphorylation.
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Multi-Immunoblotting Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
PAN-SPECIFIC AB
PHOSPHO-SPECIFIC AB
CUSTOM AB
KinasesPhosphatases
Phosphosites 4 screens
2 screens
2 screens
KCPS 1.0 - 18 antibodies tested on 1 lysate sample
KCSS 1.0 - 8 cell/tissue samples tested with up to 3 antibodies
Mass Spectrometry Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
PROTEIN IDIdentify cross-reactive proteins
PROTEINPHOSPHORYLATION
Identify phospho-sites in a protein
PROTEOMEPHOSPHORYLATION
Track hundreds ofphosphosites
KINASE SUBSTRATEPROFILING
Identify kinaseSubstrate proteins
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In Silico Prediction Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
KINASE MATCHPREDICTION
Identify kinases for a phosphosite
Obtain a specificitymatrix for a kinase
KINASE SPECIFICITYPREDICTION
PHOSPHOPROTEINMATCH PREDICTION
Identify substratesof a kinase
Identify kinases formany phosphosites
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Probe Production Services
MASSSPEC
SERVICES
IN VIVOSERVICES
KINETWORKSBLOTTINGSERVICES
KINEXMICROARRAY
SERVICES
IN SILICOSERVICES
PROBE PRODUCTION
SERVICES
SYNTHETICPEPTIDESSoluble peptides
Active enzymes and substrates
RECOMBINANTPROTEINS
PEPTIDEARRAYS
Peptides onmacroarrays
Peptides onmicroarrays
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Antibody-driven Biomarker Discovery
PREPARATION
DISCOVERY
VALIDATION
VALIDATION
LOCATION
CORRELATION
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In Vivo custom cell culture and treatment
Kinex™ Antibody Microarrays
Kinetworks™ Multi-immunoblotting
Kinex™ Reverse Lysate Microarrays
IHC Immunohistochemistry
KiNET and SigNET websites
EGF receptor 3D structure with kinase inhibitor drug Tarceva
Protein Kinase Drugs
More than 30% of world pharmaceutical research is focused on protein kinases
Over 400 human diseases linked to defects in kinase signalling
19 drugs approved and > 150 in clinical trials
Most kinase inhibitors in clinical development are for cancer treatment
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Kinexus Discovery Platform Assists All Phases of Drug Discovery and Development
DRUG ANALYSIS
DRUG TARGETDISCOVERY
DRUG DISCOVERY
DRUG TARGETVALIDATION
PRE-CLINICALVALIDATION
CLINICALVALIDATION
BIOMARKERDISCOVERY
PROTEINSCREENING
CELL/TISSUESCREENING
HTP COMPOUNDSCREENING
CELLS MECHANISM
ANIMALSTOXICOLOGY
HUMAN PHASE I, II + III TRIALS
PATIENTSCREENING
MASS SPECIN VIVO MULTI-BLOTMICROARRAYS IN SILICOPROBE
PRODUCTION
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Kinexus Services for Kinase Drug Discovery
KINASE DRUG SPECIFICITY
DRUG TARGETKINASE
DISCOVERYKINASE ASSAY DEVELOPMENT
KINASE SUBSTRATEDISCOVERY
PROTEINSCREENING
CELL/TISSUESCREENING
HTP COMPOUNDSCREENING
CELLS MECHANISM
Kinex™ KAM
Kinetworks™
In Silico Prediction
KiNET DB
SigNET KB In Silico - IKMP
Kinex™ KAM - CKSP
Mass Spec - MSKS
Kinex™ KPKM
In Silico - IKSP
Kinex™ - KKSM
Peptide Production
Kinase Testing - IKPT
Binding Assays - Kinex™ - KPKM
Activity Assays - KICP
KiNETDATABANK
Kinetworks™ Immunoblotting DataBase
First launched in 2006 Features results from >6,000 immunoblots Over 200,000 protein measurements Search by protein, treatment or cell/tissue Over 95% unpublished data
Online at www.kinet.ca KiNET DataBase
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PhosphoNET KnowledgeBaseFree, online access>92,000 confirmed and 560,000 predicted
human phosphorylation sites>23,000 human proteinsFunctional information for ~2% of phosphositesEvolutionary analysesKinase prediction
SigNETKNOWLEDGE-
BANK
SigNET KnowledgeBank
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Online at www.phosphonet.ca
www.kinexus.ca
ADVISORY BOARD
Kinexus Bioinformatics Corp.Suite 1, 8755 Ash StreetVancouver, B.C.Canada V6P 6T3
Phone: 1-604-323-2547Facsimile: 1-604-323-2548E-mail: [email protected]
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