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Overview: Genetic Mapping
Basic Principles
Genetic Mapping in Experimental Organisms
Genetic Mapping in Humans
Genes, RFLPs, SNPsMeiosis, Independent Assortment, Linkage and Crossing Over
Two- and Three-point crossesComplementation Analysis
Pedigree Analysis
Function of Human Genes
Fig. 20-13Pg. 497
Genetic Map of Drosophila
Fig. 5-14
Fig. 2-8
Gametes(haploid cells)
Crossing Over Occurs Between DNA of Homologous Chromosomes
Paternal
Maternal
PairedHomologousChromosomes
A
A
a
a
b
b
B
BaB
Ab
Pairing of Homologous Chromosomes
P
M
Fig. 2-13, 2-14
Crossing Over Occurs Between DNA of Homologous Chromosomes
1168 Family Pedigrees with at least two ASD individuals
Neurexin Proteins
Pedigree for Inheritance of Blood Type and Nail-Patella Syndrome
GelElectrophoresis
I
II
Pedigree for Inheritance of ASD and a DNA Marker
M LASD
~ 2 cM
orf1 orf2 orf3 orf4
~ 500,000 bp
Band11q13
Chromosome 11
Population Genetics (Chapter 25)
Mendelian Genetics Applied to Populations
The Hardy-Weinberg Equation (p2 + 2pq + q2)
Test populations for microevolutionEstimate frequency of carriers for recessive genetic disordersAssess effect of natural selection and mutation on microevolutionAssess effect of inbreedingDNA Profiling (“DNA fingerprinting”)
HIV-1 Structure
(Gp120)
HIV Infection Pathway
Protein encodedby CCR5-1 allele(CCR5-1 / CCR5-1 or CCR5-1 / CCR5-∆32)
HIV-1
Gp120
Cytosol
Extra-cellularSpace
Membrane
CCR5
CD4
CCR5 RFLP
CCR5-1
CCR5-∆32
CCR5 Genotype Analysis
Fig. 25-3
CCR5-∆32 Allele Frequency
Fig. 25-4
Assumptions of the Hardy-Weinberg Equation
1. Individuals of all genotypes have equal rates of survival and reproductive success (no selection).
2. No new alleles are created or converted from one allele toanother by mutation.
3. Individuals do not migrate into or out of the population(no gene flow).
4. The population is infinitely large (no genetic drift).
5. Individuals in the population mate randomly (no inbreeding)