Our Microscope 6 th Grade Science Certain materials are included under the fair use exemption of the U.S. Copyright Law and have been prepared according to the multimedia fair use guidelines and are restricted from further use. Photo by D. Tresten, 2002 Compound microscope
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Our Microscope 6 th Grade Science Certain materials are included under the fair use exemption of the U.S. Copyright Law and have been prepared according.
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Our Microscope
6th Grade Science
Certain materials are included under the fair use exemption of the U.S. Copyright Law and have been prepared according to the multimedia fair use guidelines and are restricted from further
use.
Photo by D. Tresten, 2002
Compoundmicroscope
Eye piece
Body tube
Base
Coarse adjustment knob
Arm
Fine adjustment knob
Revolving nosepiece
Diaphragm
Light source
Stage opening
Stage clips
Stage
Magnify 10 X
Objectivelens
IMPORTANT!Watch How Light Travels Through a
MicroscopeNote: Just study the vertical (up and down) portion of the light
beam! The horizontal (right/left) portion is not in our microscope.
Molecular Expressions Microscopy Primer: Anatomy of the Microscope - Transmitted Light Microscopy Optical Pathways: Interactive Java Tutorial
To find the total magnification at which a microscope is set, just multiply the power of the eyepiece (10x) times the power of the objective that is in place.
Click to practice finding total magnification…
Magnification: Multiply
If…Eye piece 10 X
Objective Lens 4 x
Total magnification is 40 X
Practice calculating Total Magnification:
If the eye piece is 10 x and the objective lens is 40 x the
magnification is...
If the eye piece is 10 x and the objective lens is 20 x the
magnification is...
If the eye piece is 10 x and the objective lens is 10 x the
magnification is...
400x
200x
100x
Microscopes are not toys!!
They are expensive tools!!
Microscope Rules
1. Always use two handsto carry a microscope!
One hand goes under the baseand one on the arm.
2. Do not touch the lenses. If they are dirty, only clean with special lens paper.
3.Be cautious when handling glass slides. Notify teacher if a slide breaks.
(Students should not handle broken glass.)
4. Always keep the scope away from the _______ of the_________.
Care of the MicroscopePage 3 of 3
edgetable
How to Use the Microscope
1. STAGE down! (away from objectives)
2. Plug in the microscope.3. Twist 4x (Low-Power) OBJECTIVE until it
clicks into place! (using nosepiece NOT objectives)
4. Stage CLIPS out!5. SLIDE on6. Stage CLIPS back in (carefully placed on top
of slide)
7. Switch on LAMP
Before using the Scope, Wake It Up:
Use of the MicroscopePage 1
1. Make sure that the
stage is all the way
DOWN and slide
is in place.
Down
In place
2. Set Scope to Low Power (4x)
In other words, set the 4x objective in place over the stage opening.
4x Objective
3. Turn the coarse adjustment knob to raise the stageto focus the slide.
4. Next, turn the fine adjustment knob until the specimenis in sharp,clearfocus.
Use of the MicroscopePage 3
5. If there is too little light, rotate the disc diaphragm to another opening.
How to Focus on a Specimen, Cont’d:
Close-up of disc diaphragm(as seen from underneaththe stage)
Do you see the largest hole that lets the most light pass? Which hole transmits the least light?
What is this?
“Field of Vision” in a Microscope
Pointer in eyepiece
Always put our microscopes “to bed” after labs:
Use of the MicroscopePage 4
1. Lamp off
2. Unplug
3. Stage down (Away from objectives)
4. All slides are removed
5. Clips IN
6. Clean stage
7. Dust cover ON
8. Put all slides and other materials in proper places