Origin of a Species: History and observations of one high throughput crystallization laboratory J. R. Luft, R. J. Collins, S. M. Gulde, A.M. Lauricella, C. A. Mancuso, J. L. Smith, C. K. Veatch, and G. T. DeTitta Recent Advances in Macromolecular Crystallization Le Bischenberg, France May 8-11, 2005
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Origin of a Species: History and observations of one high throughput crystallization laboratory J. R. Luft, R. J. Collins, S. M. Gulde, A.M. Lauricella,
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Origin of a Species: History and observations of one high throughput
crystallization laboratory
J. R. Luft, R. J. Collins, S. M. Gulde, A.M. Lauricella, C. A. Mancuso, J. L. Smith,
C. K. Veatch, and G. T. DeTitta
Recent Advances in Macromolecular CrystallizationLe Bischenberg, France
May 8-11, 2005
Hypothesis
By executing a large number of precipitation reactions for a large number of proteins we could predict crystallization conditions for a previously un-crystallized protein by comparison of precipitation behavior.
Protein Binary Code PSSUnknown 1011010010110010 --
Protein B 0111010111010010 11Protein A 0001100101010101 5
What is the most efficient way to execute precipitation reactions?
• Batch, in particular microbatch in a capillary– Minimize solution volume requirements for
precipitation cocktails
Good, but not Great• Maximum throughput (manual)
50 experiments/technician/day• Crystals were a ‘by-product’.