Organism

OrganismH2

μmol/ mg drywt/h

Growth conditions H2 evolution assay conditions

Anabaena cylindrica B-629 0.103 Air + CO2 (5%); 7000 lx at the, surface of the culture vessels

Ar + CO2 (3%); 4000 lx at thesurface of the culture vessels

Oscillatoria brevis B-1567 0.168 Air + CO2 (5%); 7000 lx at the surface of the culture vessels

Ar + CO2 (3%); 4000 lx at thesurface of the culture vessels

Calothrix scopulorum 1410=5

0.128 Air + CO2 (5%); 7000 lx at thesurface of the culture vessels

Ar + CO2 (3%); 4000 lx at thesurface of the culture vessels

Calothrix membranacea B-379

0.108 Air + CO2 (5%); 7000 lx at thesurface of the culture vessels

Ar + CO2 (3%); 4000 lx at thesurface of the culture vessels

OrganismMaximum hydrogen evolution

Growth conditions H2 evolution assay conditions

Anabaena sp. N-7363

36 μmol/mg chl a/h

- Ar

Anabaena variabilis PK84

167.60 μmol/mg chl a/h

Ar(73%) + N2(25%) +CO2(2%); 90µE m2s-1

Ar(93%) + N2(5%) +CO2(2%); 90 µE m2s-1

Anabaena variabilis PK17R

59.18 μmol/mg chl a/h

Ar(73%) + N2(25%) +CO2(2%); 90 µE m2s-1

Ar(93%) + N2(5%) +CO2(2%); 90 µE m2s-1

Anabaena variabilis AVM13

68 μmol/mg chl a/h

Air + CO2(1%);100 µE m2s-1

-

Organism used - Rhodobacter sphareroidesMedium used – asy medium Culture was harvested - 4000 rpm for 20mins Cultivated in gl medium [10 mM sodium glutamate 83 mMsodium lactate 1.5 mMsodium hydrogen carbonate ]Outcome Hydrogen production After hydrogen production was detected the culture was changed to gl medium with FeSO4 limitation

Photo fermentation for malate for hydrogen production