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Optical Tweezers Lincoln Connor Maria Conneran
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Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Dec 18, 2015

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Page 1: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Optical Tweezers

Lincoln ConnorMaria Conneran

Page 2: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Outline

• Project Goals• Optical tweezing theory and results• Sample preparation• Quadrant photodetector motivation and

results• Future work

Page 3: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Goals

• CCD imaging of optical trapping• Implement quadrant photodetector• Reorient detection wing• Manipulate biological sample

Page 4: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Setup

Page 5: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Optical Tweezing Theory

• Different indices of refraction• Conservation of momentum• Equilibrium

R.Koebler optical trap/optical tweezer: explanation with unfocused laser

Page 6: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Applications for Optical Tweezers

• Hold, measure/apply known force, local viscosity;

Parthasarathy Lab: McIntyre Lab:

Page 7: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Optical Trapping

• Beam expander • Overfill back aperture • Video

Page 8: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Sample Preparation

• Spacer• Sealants• Cleaning • Dilution

• Sticky tape wells• Higher viscosity immersion oil

Page 9: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Dilution

• 1/1600

Page 10: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Quadrant Photodetector • Silicon (400-1050nm)• X, Y, SUM• 150kHz bandwidth• Responsivity: 0.45A/W• Dark Current of 5nA• Recommended spot size: 1 mm to 3.9 mm

Page 11: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Calibration

5.37μm/V

Piezoelectric Voltage

Page 12: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Equipartition12𝑘𝑏𝑇=

12𝑘<¿

Page 13: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Lorentzian Fit

𝑘= 𝑓 2𝜋𝛽

Page 14: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Suggestions for future work

• Improve sample preparation• Improve stability• Detection beam• SLM implementation

Page 15: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Questions?

Page 16: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Horizontal Setup

Page 17: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Lens System for QPD

Page 18: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.
Page 19: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Data Acquisition

Page 20: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

QPD DAQ

Page 21: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

GigE Camera

Olympus 100x oil immersionobjective lens

Page 22: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

Einstein-Ornstein-Uhlenbeck

Page 23: Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.