490 Series Biological Microscope OPERATOR’S MANUAL READ THIS MANUAL BEFORE USING THE MICROSCOPE
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490 Series
Biological Microscope
OPERATOR’S MANUAL
READ THIS MANUAL BEFORE USING THE MICROSCOPE
2
Contents 490 SERIES
Before use .............................................................................................. 1. Nomenclature .................................................................................... 2. Operation ............................................................................................ 2-1 Assembly ........................................................................... 2-2 Setting up .......................................................................... 2-3 Focusing ............................................................................. 2-4 Adjusting the condenser and diaphragm ............. 2-5 Using the trinocular port ............................................. 2-6 Setting the stage’s stop limit ...................................... 2-7 Adjusting focusing tension ......................................... 2-8 Changing the bulb ......................................................... 2-9 Changing the fuse .......................................................... 2-10 Maintenance ....................................................................3. General speci�cations ....................................................................4. Con�guration drawing ...................................................................5. Optional Accessories .......................................................................6. Parameters/technical terms ......................................................... 6-1 Objectives .......................................................................... 6-2 Eyepieces ........................................................................... 6-3 Electrical system .............................................................. 6-4 Parameters ........................................................................ 6-5 Technical Terms ...............................................................7. Troubleshooting ...............................................................................
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Before use 490 SERIES
IntroductionCongratulations on the purchase of your new AmScope Microscope! This manual is designed for the B490 and T490 series microscopes. Though both have identical bases, the B490 is equipped with a binocular head (2 eyetubes) and the T490 is equipped with a trinocular head (2 eyetubes & 1 photo/video port). Please take a few minutes to familiarize yourself with the features and functions of your new microscope. If you want more information on microscope, parts, and accessories, please visit our website at:
www.AmScope.comWe recommend that you study this manual thoroughly before operat-ing the microscope, and that you keep it on hand for future reference. If you have additional questions or need assistance, please send us an email at:
[email protected] include the microscope model number in your email so that we can identify your model and provide immediate help.
Precautions(1) As the microscope is a precision instument, always handle it with care, avoiding impact or abrupt movement during transportation. Do not shake the package.
(2) Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry and clean place with temperatures between 32°-100° F (0°-40°C), maximum relative humidity: 85%
(3) Avoid touching the lenses on the objective and the eyepiece so that oil and dirt from your �ngerprints do not obstruct your view.
(4) Before turning the power on, make sure that the power supply voltage is consistent with the voltage of your microscope
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1. Nomenclature 490 SERIES
Nosepiece
Objective
Mechanical Stage
Condenser, iris diaphragm
Base lens
Base
Eyepiece
Fig. 1: B490
Diopter
Fine focusing knob
Coarse focusing knob
Arm
Binocular Gemel head
Iris adjustment slider
Filter holder
Slide controls
Power switch
Dimmer
Tension knob
Eyetube base
Head -lock screw
5
1. Nomenclature 490 SERIES
C-mount
Fig. 2: T490
Adjustable trinocular port
Trinocular Gemel head
Condenser adjustment knob
Limit-stop knob
Coarse focusing knob
Fine focusing knob
Condenser screw
6
1. Nomenclature 490 SERIES
Fig. 3: Bottom
Fig. 4: Condenser, iris and �lter holder
Fuse Light screw
Halogen bulb
Iris Filter holder
Condenser screw Condenser
Iris adjustment slider
De�nitions:
Base Lens Directs the light source towards the slideCoarse Focusing Knob Used to initially bring the slide into sight and
focus
Adjustable Trinocular Port Threaded port with adjustable length for attachment of digital or video camera
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1. Nomenclature 490 SERIES
Condenser & Iris Diaphragm Controls the amount of light that hits the slide
Condenser Adjustment Knob Moves the condenser up and down
Condenser Screw Screws the condenser in place, secureing it to the mechanical stage
Dimmer Controls the amount of light that escapes from the base lens
Diopter Allows the focus to be perfected for both eyes, independent of each other
Fine Focusing Knob Used to re�ne focus to a clear image Head Lock Screw Screws the microscope head into place
Limit-Stop Knob Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective (adjustable).
Mechanical Stage Mechanically moves the slide along an X and Y axis for optimal positioning
Nosepiece Houses the objective lenses Rotating Gemel Head Adjusts eyepieces to �t the distance between
the user’s eyes for added comfort
Tension Knob Adjusts the tension of the focusing knobs
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2. Operation 490 SERIES
2-1 Assembly (1) First take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which side is labeled up. Remove tape and open the container carefully so as to avoid dropping and damaging the optical items. Check carefully to ensure that all parts and accessories are intact.
(2) Check the parking list below: One Microscope Body One Binocular (B490) or Trinocular (T490) Rotating Gemel Head One Adjustable Trinocular Photo Port (for T490 only) Four Objectives: 4X, 10X, 40X, and 100X Two Pairs of Eyepieces: W10X and P16X Color Filters One Bottle of Immersion Oil One 6V/20W Halogen Spare Bulb One Spare Fuse One Dust Cover
(3) Remove the microscope body from the box and remove the plastic protective covering. The body of the microscope is composed of the base, the stage, the arm, and the nosepiece.
(4) Loosen the metal knob located directly above the nosepiece (the head-lock screw) completely. Place the head, �at circular side down, onto the circular opening at the top of the arm. Make sure the microscope head is securely on top of the arm and tighten the head-lock screw to lock the head in place.
(5) For the T490 model only, screw the photo port onto the top of the microscope head.
(6) Remove the eyetube caps and drop the desired eyepieces into the eyetubes without touching the lens.
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2. Operation 490 SERIES
(7) Screw the objectives into the microscope nosepiece from the lowest magni�cation to the highest, being careful not to touch the lens.
(8) Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob on the side of the base.
2-2 Setting up (1) Loosen the head-lock screw and adjust the microscope head so that it is in a comfortable position for observation. Lock the head-lock screw. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyetubes and rotating the eyepieces either towards or away from one another, until one circle of light can be seen.
(2) Place the specimen to be studied on a glass slide (or simply use a prepared slide), and place it on the stage, holding it sungly in place with the metal slide-holders of the mechanical stage. Using the slide controls, center the specimen over the stage opening, lining it up with the light and objective lens.
(3) To adjust the illumination, slowly turn the dimmer until the desired intensity of light is achieved.
2-3 Focusing (1) Turn the nosepiece to choose an objective. It is easiest to use the lowest magni�cation �rst (usually 4X) to locate and focus the specimen. As you move up in magni�cation, you may have to refocus the image a little bit each time. When using the 100X objective, a little immersion oil must be put between the objective and the cover glass on the slide in order to minimize the distortion cause by air. Always wipe the oil o� the objective with either a glass-cleaning paper or cloth after each use.
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2. Operation 490 SERIES
(2) Begin focusing by �rst looking with one eye through the eyetube without the diopter. Close your other eye. Loosen the limit-stop knob (located on the inside of the coarse-focusing knob) in order to give the mechanicalstage a full range of movement and adjust the coarse-focusing knob to bring the slide into focus. You may also need to re-center the specimen on the stage.
(3) When the object is in your �eld of vision, adjust the �ne-focusing knob until you see a clear, sharp image. BE ALERT: When moving the mechanical stage vertically, be careful not to hit the objective lens with the slide. Remember to use the limit-stop knob to avoid impact between the objective and the slide when using the 40X and 100X objectives. Lock the limit-stop knob at the position where the slide almost touches the objective.
(4) Once the image is focused through the one eyepiece, look through the other eyepiece and turn the diopter until the image is clear through that eyepiece as well
2-4 Adjusting the condenser and diaphragm (1) Using the condenser-adjustment knob, you can change the distance between the light condenser and the stage. This allows you to control the concentration of the light hitting your slide.
(2) By Changing the aperture of the iris diaphragm, you can adjust the background brightness. Adjust the aperture of the iris diaphragmm using the iris adjustment slider located directly under the stage.
(3) If you want to use a color �lter, swivel the �lter holder out, towards you. You can now place the desired color �lter into the circular opening. Slide the �lter holder back to original position before observation.
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2. Operation 490 SERIES
2-5 Using the trinocular port The AmScope T490 is uniquely designed so that you can focus the image through the eyepieces and the trinocular port simultaneously. This “parfocal” feature allows the images through the microscope’s eyepieces, and those displayed on your computer screen or TV to be viewed at the same time! You do not need an adapter to attach your AmScope camera to the trinocular port. However, you may need an adapter if you have a non-AmScope camera.
Attaching a camera:(1) If your camera has a C-mount, simply screw the camera onto the trinocular port.
(2) If your camera has a 23mm mounting size, remove the C-mount from the top of the trinocular port by loosening the screw. Then, drop the camera into the trinocular tube.
(3) To focus through the trinocular port, simply turn the middle portion of the tube.
2-6 Setting the stage’s stop-limit (1) Set the stage to the maximum height desired.
(2) Pull the stop-limit knob towards the stage. The stage’s stop-limit is now set, allowing you to move the stage down, but not up past this point.
(3) To reset the stage’s stop-limit, simply push the stop-limit knob back towards the back panel of the microscope. The stage’s full range of motion is now restored.
12
2. Operation 490 SERIES
2-7 Adjusting focusing tension(1) To adjust the tension of the focusing knobs, �rst locate the black ridged tension-knob on the inside of the coarse focusing knob.
(2) To decrease tension, rotate the tension adjustment forward, towards the stage. To increase tension, rotate the tension adjustment backwards, towards the back panel of the microscope.
2-8 Changing the bulb(1) Before changing the light bulb, �rst pull the plug out of the electrical socket and wait for the lamp to cool down in order to avoid being burned. Also, make sure to remove the eyepieces or secure them to the microscope before turning the microscope over!
(2) Loosen the door-screw on the bottom of the microscope and pull open the small door. Pull the old bulb out and replace it with a new one. Close the door and tighten the screw.
2-9 Changing the fuse(1) First, remove the eyepieces or secure them to the microscope before turning the microscope over.
(2) Loosen the door-screw on the bottom of the microscope and pull opUsing a �at-head screwdriver, unscrew the fuse located on the bottom of the microscope.
(3)Remove the old fuse and replace with a new one. Screw the fuse back in place.
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2. Operation 490 SERIES
2-10 Maintenance (+) All glass surfaces must always be kept clean. Fine dust on the optical surface should be blown o� using a hand blower or gently wiped o� with a soft lens tissue. Carefully wipe o� oil or �ngerprints on lens surfaces using tissue moistened with a small amount of 3:7 alcohol to ether mixture
(+) Do not use this solution to wipe the surface of other components. These parts, especially those made of plastic, should be cleaned with a neutral detergent.
(+) Do not assemble or disassemble the microscope’s electrical compo-nents yourself.
(+) After use, cover the microscope with the provided dustcover. Make sure to keep it in a dry and clean place in order to prevent rust.
14
3. Speci�cations 490 SERIES
Speci�cations for the 490 Series Biological Microscope
PARTS SPECIFICATIONS WF10X/18mm
WF10X/18mm with pointer WF10X/18mm with reticle
WF16X/13mm
WF Eyepiece
WF20X/10mm X5P X01P
Plan Eyepiece
X61P4X/0.10
10X/0.25 40X(spring)/0.65 60X(spring)/0.85
DIN Achromatic Objectives
100X(spring, oil)/1.25 X4 X01
40X(spring) Plan
Objectives 100X(spring, oil)
Binocular sliding head, 360° swiveling
45° Viewing Head Trinocular sliding head, 360° swiveling
Binocular Gemel head, 360° swiveling 30° Viewing Head Trinocular Gemel head, 360° swiveling
Trinocular Port
C-mount adjustable photo port.
Simultaneous Viewing
Focusing Coaxial focusing system Nosepiece Quadruple nosepiece
Stage 130mm x 140mm mechanical stage Moving range: 50mm x 76mm
Condenser Abbe, NA=1.25 Diaphragm Iris diaphragm
Halogen light with dimmer Illumination ф50mm mirror
Lamp 6V/20W Filter Blue/yellow/green
B490 T490
23mm adjustable photo port.
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4. Con�guration drawing 490 SERIES
CCD
VID
EO
CAM
ERA
400P
H
490T
H30
G
400T
H45
49
0BH
30G
40
0BH
45
400M
H45
400M
S
400B
L 40
0MR
400N
P4
400F
B
A10
0X
A40
X A
10X
A4X
PA10
0X
PA60
X P
A40
X P
A10
X P
A4X
WF1
0X18
W
F10X
18P
WF1
5X16
W
F20X
10
EP5
EP1
0 E
P16
490
Seri
es B
iolo
gica
l M
icro
scop
e Co
n�gu
ratio
n dr
awin
g
400C
D1.
25
DRY
100
OIL
100
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5. Optional Accessories 490 SERIES
NAME DESCRIPTION MODEL # PURPOSES
5X EP5X23 For obtai ning 20X, 50X, 200X and 500X magni�cation powers
20X EP20X23 For obtaining 80X, 200X, 800X and 2000X magni�cation powers
25X EP25X23 For obtaining 250X and 2500X magni�cation powers
10X with pointer EP10X23P For easier identifying of objects
Eyepiece
10X with reticle EP10X23R For measuring objects 2X A2X For obtaining 20X and 32X
magni�cation powers 5X A5X For obtaining 50X and 80X
magni�cation powers 20X A20X For obtaining 200X and 320X
magni�cation powers 60X A60X For obtaining 600X and 960X
magni�cation powers Plan 4X PA4X
Plan 10X PA10X Plan 40X PA40X
Objective
Plan 100X PA100X
For obtaining high quality high resolution images
Dry Dark�eld Condenser
DK-DRY100 For obtaining low power dark�eld images
Dark�eld
Condenser Oil Dark�eld Condenser
DK-OIL100 For obtaining high power dark�eld images
CMOS Digital
MD400 (350K) MD600 (1.3MP) MD700 (2.0MP) MD800 (3.0MP) MD900 (5.0MP)
For obtaining still or live digital images
CCD Digital MD1000 For obtaining high quality still or live digital images
Camera
CCD TV Video CCD-NP
Optional AccessoriesThere is a variety of accessories available that can enhance your microscope’s capabilities and give it additional features. Some of them are listed in the table below:
For live images on TV
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6. Parameters/Technical terms 490 SERIES
Aluminum case AC-400 For carrying microscope around Case Hard wood case WC-400 For storage
Simple PCS Phase contrast images Phase Contrast Kit Turret PCT High quality phase contrast
images Stage Warmer TCS-100 For controlling stage
temperature Fluorescent
Kit FK-YX Fluorescent images
Please �nd more accessories on our website at: www.AmScope.com
6-1 Objectives
6-2 Eyepieces
Type Magni�cation Numerical Aperture
(N.A.) Medium
Par-focal Distance
(mm)
Magni�cation Marks
(color ring) 4X 0.10 Air 45 Red
10X 0.25 Air 45 Yellow 40X 0.65 Air 45 Light blue 60X 0.85 Air 45 Deep blue
DIN Achromatic Objective (195mm)
100X 1.25 Cedar oil 45 White
4X 0.10 Air 45 Red 10X 0.25 Air 45 Yellow 40X 0.65 Air 45 Light blue
Plan Objective (195mm)
100X 1.25 Cedar oil 45 White
Type Wide �eld eyepiece Plan eyepiece Magni�cation 10X 15X 20X 5X 10X 16X Field of view
(mm) ф18 ф13 ф11 ф18 ф18 ф15
Optional Accessories cont’d...
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6. Parameter/technical terms 490 SERIES
6-3 Electrical system There are two options for electrical systems. Both are equipped with 6V/20W halogen lamps.
(1) 220V～240V power supply: 220V～240V ±10%, 50Hz This electrical system is CE and GS certi�ed
(2) 100V～120V power supply: 100V～120V ±10%, 60Hz This electrical system is UL certi�ed
6-4 Parameters(1) Total magni�cation: 20X～1600X
(2) Field of view: ф0.8mm～ф4.5mm
(2) Mechanical tube length: 160mm
(4) Object to primary image distance: 195mm
(5) Fine focusing sensitivity: 0.002mm
6-5 Technical terms(1) Total magni�cation= magni�cation of objective multiplied by magni�-cation of eyepiece
(2) Field of view= line field of view of the eyepiece divided by the magnification of the objective
(3) Object to primary image distance= n·sinα (max), N.A. is an important parameter that marks the features of the objective and condenser. The “n” is the refractive index of the medium (air or immersion oil) between the
·
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6. Parameter/technical terms 490 SERIES
objective lens and the specimen. The “α ” is 1/2 the aperture angle. The larger the N.A., the high the resolution of the objective
(4) Object to primary image distance = the distance between the object plane to the primary image plane. The conjugate distance is �xed
(5) Mechanical tube length = the distance between the objective shoulder and the ocular shoulder
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7. Troubleshooting 490 SERIES
Troubleshooting for the 490 Series Biological Microscope
O P T I C A L I S S U E S
The nosepiece is misaligned
There is no cover glass on the slide The cover glass is not standardThe specimen is upside down
SYMPTOM CAUSE REMEDY
One side of the �eld of view is
darker
Turn the nosepiece until it clicks into place
Stain or dust has accumulated on the condenser, object, eyepieces, or base lens
Obstructions are observed in
the �eld of view
Clean the lens
Stains has accumulated on the specimen
Stains have accumulated on the lens
Clean the specimen
Clean the lens
Unclear Image
The immersion oil has accumulated on the dry objective
No immersion oil used with the 100X objective
Air bubble in the immersion oilUsed wrong oilThe aperature is not open to an appropriate diameter
Stain or dust has accumulated on the lens in the inlet of the head
The condenser is not in the right position
Add the cover glassUse glass w/0.17mm thicknessFlip it over
Clean lens thoroughly
Use immersion oil
Pop the air bubbles
Adjust the iris diaphragm
Use standard cedarwood oil
Clean the lens
Adjust the condenser
One side of the �eld of view is
dark or the image moves
while focusing
The �eld of view is not
bright enough
The specimen slide is not �xed
The nosepiece is not in the right position
The iris diaphragm is not big enough
The condenser is not in the right position
Stain or dust has accumulated on the condenser, objective, eyepieces, or base lens
Secure slide to the stage with clips
Turn the nosepiece until it clicks into place
Adjust the diaphragm
Adjust the condenser
Clean the lens
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7. Troubleshooting 490 SERIES
Troubleshooting for the 490 Series Biological Microscope cont’d...
O P T I C A L I S S U E S
The mechanical stage is not properly secured
Tension knob is too tight
SYMPTOM CAUSE REMEDY
The color of the image is not
accurate
Adjust the brightness-adjustment knob
No �lter is used
The objective touches the cover glass
Use desired �lter
The slide is not secured correctly Adjust the slide
The coarse-focusing knob won’t raise the
stage
The tension knob is too loose
Limit-stop knob locked
Limit-stop knob locked
Tighten the mechanical stage
Loosen it a little
Tighten it a little
Loosen the limit-stop knob and lock it after refocusing
The microscope
does not light up
The bulb burns out frequently
The bulb is not inserted correctly
The bulb burned out
The fuse burned out
The voltage is too high
Used wrong bulb
Insert it correctly
Replace the bulb
Replace the fuse
Use correct power supply
Replace w/correct bulb
The brightness-adjustment knob is not in the right position
M E C H A N I C A L I S S U E SThe cover glass is upside down
The cover glass is not standard Use glass w/0.17mm thickness
Flip the cover glass over
Unable to move the slide
smoothly
The microscope is unplugged
Check the power cable ‘s plug
Focus-knob does not turn
Stage declines by itself
The �ne-focusing knob won’t raise the
stage
Loosen the limit-stop knob and lock it after refocusing
E L E C T R I C A L I S S U E SThe bulb �ickers The bulb is close to burning out Replace the bulb
The fuse burns out frequently
The voltage is too high Use the correct power supply or get a voltage adapter
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