Online Resource 2 (A) 10 µm (L ) (R) Title:Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments Authors:Liyuan Hou 1 , Hyunsun Park 1,2 , Shigeru Okada 2,3 , Takeshi Ohama 1,2, * 1 School of Environmental Science and Engineering, Kochi University of Technology (KUT), Tosayamada, Kochi 782-8502, Japan 2 JST, CREST, 5 Sanbancho, Chiyoda, Tokyo 102-0075, Japan 3 Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan Corresponding author:Takeshi Ohama E-mail: [email protected]. Protoplasma