On-Chip pH Sensing with Gel Microbea d Positioned by Optical Tweezers Hisataka Maruyama 1 , Fumihito Arai 2 and Toshio F ukuda 1 1 Department of Micro-Nano Systems Engineering, Nagoya Unive rsity, Japan 2 Department of Bioengineering and Robotics, Tohoku Universi ty, Japan 9633584 黃黃黃 Ref: μTAS2006 PME MAPS
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On-Chip pH Sensing with Gel Microbead Positioned by Optical Tweezers
PME MAPS. On-Chip pH Sensing with Gel Microbead Positioned by Optical Tweezers. Hisataka Maruyama 1 , Fumihito Arai 2 and Toshio Fukuda 1 1 Department of Micro-Nano Systems Engineering, Nagoya University, Japan 2 Department of Bioengineering and Robotics, Tohoku University, Japan. - PowerPoint PPT Presentation
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On-Chip pH Sensing with Gel Microbead Positioned by Optical Tweezers
Hisataka Maruyama1, Fumihito Arai2 and Toshio Fukuda1
1Department of Micro-Nano Systems Engineering, Nagoya University, Japan2Department of Bioengineering and Robotics, Tohoku University, Japan
9633584 黃紫郁Ref: μTAS2006
PME MAPS
2007/11/28 2
Outline
• Introduction
• Gel-Tool Contained a pH Indicator
• Experimental
• Conclusions
• References
PME MAPS
2007/11/28 3
Introduction
• Environment measurement in a microchip is important to the on-chip experiment.
• Conventionally, microsphere’s surface is modified by fluorescent reagents and indicators.
• A local pH sensing method in a microchip using a functional gel-tool was developed.
• Gel-tools are UV curing and adhere to the glass, but we can manipulate them by the optical tweezers.
• Measure pH value locally from the color of gel-tool using calibrated color information in YCrCb color.
PME MAPS
2007/11/28 4
Gel-Tool Contained a pH Indicator• Gel-tool is made by salting-out of ENT-3400 (polyet
hyleneglycol, PEG) which is hydrophilic resin.
• Manipulate gel-tool by optical tweezers in a water because the relative refractive index of PEG (1.42) is higher than that of water (1.33).