Saratov State University ______________________________________________ Department of Optics & Biophotonics __________________________________________________ Saratov Fall Meeting 2011 Ocular drugs influence on the structure of ocular tissues Anzhelika S. Orlova, Tatyana G. Kamenskikh, Ivan D. Kamenskikh, Igor O. Kolbenev, Saratov State Medical University Alexey N. Bashkatov, Elina A. Genina, Artem A. Aratov, Anastasiya M. Parkheychuk, Valery V. Tuchin Department of Optics and Biophotonics of Saratov State University e-mail: [email protected]
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Ocular drugs influence on the structure of ocular tissues
Ocular drugs influence on the structure of ocular tissues. Anzhelika S. Orlova, Tatyana G. Kamenskikh, Ivan D. Kamenskikh, Igor O. Kolbenev, Saratov State Medical University Alexey N. Bashkatov, Elina A. Genina, Artem A. Aratov, Anastasiya M. Parkheychuk, Valery V. Tuchin - PowerPoint PPT Presentation
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It is well known that diagnostics and treatment of many diseases of human eye is connected with monitoring of glucose content. However, in spite of numerous investigations dealing with the transport of the metabolite within biological tissue, the problem of monitoring of structural changes of eye cornea under action of glucose has not been studied in detail.
Goal of the study is investigation of influence of 40% aqueous glucose solution on stuctural changes in human eye cornea at the noninflamation cornea edema after post-surgical phacoemulsification of aging cataract
It is well known that diagnostics and treatment of many diseases of human eye is connected with monitoring of glucose content. However, in spite of numerous investigations dealing with the transport of the metabolite within biological tissue, the problem of monitoring of structural changes of eye cornea under action of glucose has not been studied in detail.
Goal of the study is investigation of influence of 40% aqueous glucose solution on stuctural changes in human eye cornea at the noninflamation cornea edema after post-surgical phacoemulsification of aging cataract
Cornea structure has been investigated using Heidelberg Retina Tomograph (HRT) with the Rostok Cornea Module (RCM) (Heidelberg Engineering GmbH, Germany)
Cornea structure has been investigated using Heidelberg Retina Tomograph (HRT) with the Rostok Cornea Module (RCM) (Heidelberg Engineering GmbH, Germany)
Sixty volunteers were enrolled for the study. The subjects were divided into two groups. The I group included thirty volunteers (21 male and 9 female at the age from 56 to 72 years) with post-surgical edema of cornea after phacoemulsification of aging cataract. The II (control) group included thirty health volunteers (16 male and 14 female at the age from 22 to 30 years).
For all persons the study of corneal structure and thickness was performed before the instillation of 40%-glucose solution (2 drops 0.05 ml = 0.1 ml) and in 20 and 40 minutes after the procedure with HRT and OCT
Sixty volunteers were enrolled for the study. The subjects were divided into two groups. The I group included thirty volunteers (21 male and 9 female at the age from 56 to 72 years) with post-surgical edema of cornea after phacoemulsification of aging cataract. The II (control) group included thirty health volunteers (16 male and 14 female at the age from 22 to 30 years).
For all persons the study of corneal structure and thickness was performed before the instillation of 40%-glucose solution (2 drops 0.05 ml = 0.1 ml) and in 20 and 40 minutes after the procedure with HRT and OCT
The comparison of hydration degree of rabbit cornea and collagen (gelatin) under action of solutions with different pH measured in different time. M is the mass of the sample; t is the time of action. The data about gelatin swelling have been obtained from Lykov A.V. Transport phenomena in capillaro-porous media / Moscow, 1954
The typical OCT images obtained from cornea in normal state under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 20 min; (c) – after 40 min
The typical HRT images obtained from corneum epithelium in normal state under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 20 min; (c) – after 40 min
The typical HRT images obtained from corneum epithelium with edema under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 20 min; (c) – after 40 min
The typical HRT images obtained from corneum endothelium in normal state under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 40 min
The typical HRT images obtained from corneum endothelium with edema under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 40 min
Russian Federation governmental contacts 02.740.11.0770, and 02.740.11.0879
Russian Federation governmental contacts 02.740.11.0770, and 02.740.11.0879
Grant #224014 Network of Excellence for Biophotonics (PHOTONICS4LIFE) of the Seventh Framework Programme of Commission of the European Communities
Grant #224014 Network of Excellence for Biophotonics (PHOTONICS4LIFE) of the Seventh Framework Programme of Commission of the European Communities
Acknowledgement:
The authors thank Dr. Alexander B. Pravdin (Department of Optics and Biophotonics of Saratov State University) for the help in preparation of the buffer solutions