The effects of kinetin and Alar-85 applications on the growth and flowering of Adonis autumnalis, L. Journal Title: Proceedings annual meeting - Plant Growth Regulator Society of America. Journal Volume/Issue: 1985. (12th) Main Author: Abdalla, N.M. Other Authors: El-Gengaihi, S.E., Solomos, T., Al-Badawy, A.A. Format: Article Language: English Subjects: height plant characteristics Adonis ornamental plants medicinal plants spraying kinetin daminozide stems diameter branching flowers yields Document details Title Influence of growth regulators on growth, herbage and essential oil yield in davana (Artemisia pallens Wall.). Authors Bhat, P. B.; Farooqi, A. A.; Subbaiah, T. K. Editors Bhattacharyya, S. C.;Sen, N.;Sethi, K. L. Conference paper Proceedings of the 11th international congress of essential oils, fragrances and flavours. New Delhi, India, 12-16 November, 1989 Vol. 3. 1990 pp. 81-84 Conference Title Proceedings of the 11th international congress of essential oils, fragrances and flavours. New Delhi, India, 12-16 November, 1989 Vol. 3. ISBN 1-85529-018-9 Record Number 19920311100
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The effects of kinetin and Alar-85 applications on the growth and flowering of Adonis autumnalis, L.
Journal Title: Proceedings annual meeting - Plant Growth Regulator Society of America.
Influence of growth regulators on growth, herbage and essential oil yield in davana (Artemisia pallens Wall.).
AuthorsBhat, P. B.; Farooqi, A. A.; Subbaiah, T. K.
EditorsBhattacharyya, S. C.;Sen, N.;Sethi, K. L.
Conference paperProceedings of the 11th international congress of essential oils, fragrances and flavours. New Delhi, India, 12-16 November, 1989 Vol. 3. 1990 pp. 81-84
Conference TitleProceedings of the 11th international congress of essential oils, fragrances and flavours. New Delhi, India, 12-16 November, 1989 Vol. 3.
ISBN1-85529-018-9
Record Number19920311100
Abstract
Gibberellic acid (GA3) was sprayed at 0, 50 and 100 p.p.m. on seedlings 20 days after transplantation to the field. Cycocel (CCC) [chlormequat]
(at 0, 2000 and 4000 p.p.m.) and TIBA (at 200, 300 and 400 p.p.m.) were sprayed once before flowering (30 days after transplanting) and a
second time after flowering (60 days after transplanting) as sub-plot treatments. Plant height, number of branches, plant spread, number of days
taken for 50% flowering and fresh and dry weight of flower heads and leaves were determined. Leaves and flower heads were air dried for 48 h
Abstract (English) Laboratory trials were carried out to investigate the effect of Accel on the postharvest vase life of Easter lily cut flowers. Flowering stems of Easter lily cultivar `St. Joseph' were purchased from a commercial farm in Gaborone, Botswana. Accel at 25, 50, or 75 mg L-1 significantly delayed postharvest catastrophic leaf yellowing of Easter lily, flower senescence and abscission, improved flower water uptake, increased flower vase life and retarded leaf chlorophyll and nitrogen degradation. The results indicated that Accel can be used as a commercial cut flower preservative solution for Easter lily and other cut flowers with postharvest leaf yellowing as a problem.
Submitted by:National Agricultural Research Centre (Pakistan)
Abstract (English) Laboratory trials were carried out to investigate the effect of gibberellic acid (GA3) on the postharvest quality and vase life of gerbera cut- flowers. Freshly cut flower stems of gerbera cultivar `Ida Red', with two outer disc florets open were put in flower vases containing 0, 2.5, 5, or 7.5 mg L-1 of GA3. The treatments were arranged in a Completely Randomized Design with four replicates. Gerbera cut-flowers held in GA3 at 2.5, 5 or 7. 5 mg L-1 significantly delayed flower senescence by increasing the number of disc florets open, delayed petal fading and abscission. Gibberellic acid at 2.5, 5 or 7.5 mg L-1 significantly reduced dry matter content in the flower heads and stems of gerbera cut-flowers. Gerbera cut-flowers held in 2.5, 5 or 7.5 mg L-1 GA3 had significantly higher water content in the flower heads and stems, hence maintaining flower turgidity, reduction in bent neck and flower senescence and increased flower quality after 14 days of holding compared to flowers held in distilled water. Gibberellic acid at 2.5, 5 or 7.5 mg L-1 has the potential to be used as a gerbera cut-flower preservative solution.
Submitted by:National Agricultural Research Centre (Pakistan)
Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
El-Saeid H. , Abouziena H. , AbdAlla M. 2011. EFFECT OF SOME BIOREGULATORS ON WHITE LUPINE (Lupinus termis) SEED YIELD AND ITS COMPONENTS AND ON ENDOGENOUS HORMONES CONTENT IN SEEDS, EJPAU 14(4), #02.Available Online: http://www.ejpau.media.pl/volume14/issue4/art-02.html
EFFECT OF SOME BIOREGULATORS ON WHITE LUPINE (LUPINUS TERMIS) SEED YIELD AND ITS
COMPONENTS AND ON ENDOGENOUS HORMONES CONTENT IN SEEDS
As for straw yield per plant, the increase was observed as a result of growth regulators
treatments except kinetin at 50 and 100 mg∙dcm-3 which decreased significantly the value of
this character (Fig. 2). On the other side, these treatments had no significant effect on crop
index, harvest index and shelling percentage. Generally, these treatments decreased those
characters, except plants sprayed with kinetin. The highest values of crop and harvest index
were obtained as a result of kinetin at 50 mg∙dcm-3, while the maximum value of shelling
percentage was obtained at 25 mg∙dcm-3. The results showed also that migration coefficient
was obtained under the effect of kinetin at 100 mg∙dcm-3.
Figure 2. Effect of some bioregulators on seed and straw yields per white lupine plant
There was significant effect of plant growth regulators application on lupine seed yield. The
maximum yield was recorded with the application of kinetin at 50 mg∙dcm-3 followed by
kinetin at 100 mg∙dcm-3 as well as IAA at 100 mg∙dcm-3 and minimum yield was recorded in
unsprayed plants (Fig. 2).
The increment of seed yield as a result to application of CK could be attributed to that
exogenous application of cytokinin to developing flowers prevents their abortion and permits
the initiation of seed development [3]. Morris [21] reported that accumulation of cytokinins
in developing seeds coincides with the highest rates of cell division, which is consistent with
the idea that CK increase the sink strength of seeds for assimilates. Matthysee and Scott [20]
stated that CK serve as a carrier from the root apex as it regulates the growth of lateral roots
and lateral branches of the shoots. These results are in agreements with those reported by
Seymour[26], Khalil et al. [15] and El-Saeid et al. [8]. Prusinski et al. [24] reported that the
seed yield of yellow lupine treated with auxin was significantly higher than when treated with
CK and the non-treated control.
Endogenous hormonal content
There is a relationship between the exogenous and endogenous hormones, especially GA3,
and there is a relationship between the end product and endogenous hormones [4]. The results
in Table (2) indicated that spraying the lupine plants with IAA or NAA at the three
concentrations used caused a significant increase of the endogenous hormones contents i.e.
GA and IAA in white lupine seeds. While application of kinetin at 50 and 100 mg∙dcm-3 led
to a significant increment of the two endogenous hormones (GA and IAA), however spraying
kinetin at the low concentration (25 mg∙dcm-3) had a slight enhancement of the endogenous
hormones contents in lupine seeds.
Table 2. Effect of some bioregulators on chemical composition of white lupine seeds (combined analysis of two seasons)
Treatments Characters
SubstancesConcentration
[mg∙dcm-3]
Migration
coefficient
Chemical contents in seeds
[ %]
Endogenous
hormones
[ng∙g-1 seeds]
total
carbohydrates
crude
proteinsoil GA IAA
Control – 0.59 24.7 39.4 15.9 25 31
IAA
100 0.61 25.2 45.3 14.7 68 99
200 0.69 25.3 45.7 14.3 95 200
400 0.61 24.7 45.5 14.9 124 300
NAA 10 0.63 24.7 43.7 15.0 61 58
20 0.65 25.8 44.7 15.0 88 90
40 0.64 24.7 43.8 15.1 107 95
Kinetin
25 0.70 25.6 48.7 14.3 32 47
50 0.77 27.7 51.6 13.0 51 63
100 0.81 29.3 50.8 12.9 66 76
LSD0.05 0.06 0.2 0.4 0.1 18 43
It's evident that the level of IAA increased endogenous GA in the seeds, such increase were
362%, 218% and 166%, compared with control seeds when plants treated with 400, 200 and
100 mg∙dcm-3 IAA, respectively. However the content of IAA in the seeds produced from
plants treated by different levels of IAA was more than the control seeds. Such increases
were 812%, 497% and 206%, when plants treated with 400, 200 and 100 mg∙dcm-3 IAA
respectively.
The amounts of GA in the seeds produced from plants treated by different levels of NAA are
shown in Table 2. It's evident that the level of NAA increased endogenous GA in the seeds,
such increase were 281%, 211% and 111%, compared with control. However the content of
IAA in the seeds produced from plants treated by different levels of NAA was more than the
control seeds. Such increases were 174%, 112% and 56%, when plants treated with 40, 20
and 10 mg∙dcm-3 NAA, respectively.
Data in Table 2 indicated that sprayed white lupine plants with kinetin at concentrations of
25, 50 and 100 mg∙dcm-3 resulted in significant increase the GA content in lupine seeds by
167%, 104% and 26%, relative to the unsprayed plants, respectively. In the same direction
the amount of IAA in the seeds was increased by 147%, 91% and 26%, respectively in
comparison to untreated plants. Similar finding was obtained by Khalil et al. [15], El-Saeid et
al. [8]. Khalil et al. [15] found that Kinetin treatments increased the level of IAA,
gibberellins and cytokine's in lentil plants.
It seems very fruitful to correlate and discuss changes in the separate endogenous groupings
of active materials existing into extracted of seeds from plants treated by different levels of
IAA, NAA and kinetin. The high levels of GA and IAA in seeds produced from plants treated
by IAA relative to those treated by NAA or kinetin may explain the increase in plant height
and number of leaves and pods. Its well known that the increase in the levels of both auxin
and gibberellins promote cell division and cell enlargement and hence increased plant height
[12]. On the other hand, high levels of auxins and gibberellins together were found to
enhance leaf production [25].
Chemical contents in seeds
Data recorded in Table (2) revealed that spraying white lupine plants with the three growth
substances i.e. IAA, NAA or kinetin at different concentrations caused an increase in resulted
in increase of total carbohydrate as well as crude protein contents, compared to unsprayed
plants. The highest significant values of total carbohydrates and protein contents were
observed under the effect of kinetin at 100 and 50 mg∙dcm-3 respectively. It's apparent from
the data that fixed oil percent was decreased as a result of applied the three bioregulators
treatments. So it could be concluded that there is a negative correlation between fixed oil and
carbohydrate as well as protein content.
Using kinetin at 25, 50 or 100 mg∙dcm-3 exhibited highly significant increments in the total
carbohydrate and protein content and decreased the oil % rather than IAA, NAA and non-
treated control treatments (Table 2). Similar findings were reported with Khalil and
Mandurah [16], Tagade et al. [29] and Khalil et al. [15]. However Prusinski et al. [24]
reported that auxin treatment had no significant effect on chemical composition or seed yield
components of yellow lupine. Leopolda and Kriedeman [18] reported that kinetin plays an
important role in protein synthesis through activation of nucleic acid synthesis, which needed
phosphorus as participate part and potassium as stimulus to enzyme system.
CONCLUSIONS
1. Application of the three bioregulators IAA, NAA and kinetin increased the number of branches as well as pods and seeds per plant resulting in the significantly enhancement of seed yield per white lupine plant.
2. The maximum seed yield was recorded with the application of kinetin at 50 mg dcm∙ -3 followed by kinetin at the dose of 100 mg dcm∙ -3.
3. There was a negative correlation between fixed oil and carbohydrate as well as protein content.
4. Spraying white lupine plants with IAA or NAA at the three concentrations used and kinetin at 50 and 100 mg dcm∙ -3 caused a significant increase of the endogenous GA and IAA hormones contents in seeds.
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H.M. El-SaeidBotany Department, National Research CenterDokki, Cairo, Egypt
Hussein Fawzy AbouzienaBotany Department, National Research CenterDokki, Cairo, Egyptemail: [email protected]
M.S.A. AbdAllaBotany Department, National Research CenterDokki, Cairo, Egypt
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