Observe the three pictures of red blood cells. 1) Draw one cell of each picture and describe the cells. 2) Predict: Which cells was placed in low, medium.

Observe the three pictures of red blood cells.

1) Draw one cell of each picture and describe the cells.

2) Predict: Which cells was placed in low, medium and high salt solution? Explain.

3) Conclude: Why is it important to have a specific salt concentration in the blood?

Before:

Placed in hypotonic, hypertonic, Isotonic solutions.

To clarify:

After:

The inside and outside solutions are isotonic to one another.

Plant cell??

Suppose your dialysis tubing is replaced with a living fish… 1.What would happen to this fish if you placed it in tap or even in distilled water?2.What would happen to this fish if you placed it in salt water?3.So, how do some fish live in freshwater, or in saltwater?

Passive and Active Transport

Across the Cell Membrane

From Paramecium to Facilitated Diffusion

Two Molecules thick membrane

Passive Transport

Transport that doesn’t use cell energy.

Molecules flow down their concentration gradient.

Cell Membrane, Passive Transport, wandering molecules

1.Simple Diffusion Example: Water , oxygen2. Facilitated DiffusionExample: Glucose, Ions

Passive Transport

Passive Transport

Channels by Stolaf

Glucose cannot pass through

Passive Active Transport!Glucose

Osmosis

EQUILIBRIUM

Diffusion:

Channels by Stolaf

Glucose Channels open in response to the hormone Insulin

Facilitated Diffusion

Tutorial: Passive and Active Transport

Glucose TransporterEQUILIBRIUM

Lets compare:1. Diffusion (of oxygen, for

exmaple)

2. Osmosis

3. Facilitated Diffusion

Comparing In terms of:

Diffusion

Facilitated Diffusion

Osmosis

H2O

O2…

oC

1.Direction 2.Energy Source3. What’s moving? 4.Protein?

Notes

+-Glucose

Copy and Identify:1.Which is going down, which up the concentration gradient?2.Predict: where will the substance go, by passive transport? Explain

B.A.

Active Transport

Transport that requires cell energy. Notes

Transport that pushes ions up / against their concentration gradient.

1. Pumps (Charged Ions)2. Endocytosis (Large particles)3. Exocytosis (Large particles)

Active Transport

Ion Pumps – ATP Antiport

Ion Pumps -

Ion Pumps

Maintain a membrane potential with a more

negative ions inside and more positive ions outside.

Active Transport Animation Tutorial

Transport Animation (password)

Na/K pump simple animation

Pumps - by Stolaf

Endocytosis

Endocytosis of LDLPhagocytosis Simplified

Endocytosis

White Blood Cell Chases Bacteria

Exocytosis

Exocytosis animation

Identify: Passive, active transport, pumps and channels.

B.A.

Try yourself - Active or Passive?

Diffusion Channel

SymportSymportAntiport

Osmosis

Transport Animation (and more!)

Contractile vacuole Movie

A living cell is put into a surrounding of 100% pure water (most hypotonic!!) –

1.Where would the water go? 2. What would be the problem for the cell?

3. How might the cell solve it?

J.

Contractile Vacuole UC DavisContractile Vacuole new

Video: Part Two

Raise The Correct Note in response to the following slides:

Passive Transport: Blue

Active Transport: Red

Both: Blue and Red

1. Going from Low to High concentration

2. Uses ATP as source of (cellular) energy

3. Small, uncharged substances

4. Proteins are involved

5. Going from High to Low concentrations

6. Ion Channels and Glucose Transporter

7. Ion Pumps

8. Uses Heat as the source of (kinetic) energy9. Osmosis is an example of….

10. Charged substances (ions).

11. Endocytosis / Exosytosis

12. Demonstrated by dialysis tubing

13. ATP

14.

ATP

15.

16.

17.

18. Oxygen Diffusion into cells

19.

20. Demonstrated by the decalcified egg experiment

21.

15.

16.

ATP

17.

19.

Transport of Proteins Inside the Cell: Vesicular Transport

Protein Transport - ER and Golgi

Problem: How do we get a new protein from the center of the cell to the membrane?

NSolution: Vesicular Transport

?

A.Endocytosis: Purpose is to take in liquids or a large particle, as a signal for the cell.

B. Exocytosis: Purpose is to release a large molecule from the cell, to get rid of waste or to release a signal protein.

Answer to journal:

The cell membrane is said to be Selective and Semi-permeable.(or ‘selectively permeable’)

Write at least three facts you learned that support this idea.

Consider a certain concentration of salt in water.This solution can be both hypotonic and hypertonic at the same time.

How is this possible?!

Hypo-tonic

to

Hyper-tonic

to

Desalination Plant.

1. Water flows in from the estuary or sea 2. Salt water contains sodium and chloride ions3. Pressure is applied to force salt water through membrane4. Semi-permeable membrane with millions of microscopic holes 5. Clean water fit for drinking6. Saline concentrate flows out

You have just learned that water moves from lower to higher solute concentration.1.How is it possible to drive water in the opposite direction? Give an example.2. Active transport – uses consumed energy. Passive transport – does not. What is desalination, then?