Objectives To determine the properties and quality of the horse joint supplements Legend and Hyalovet by: •Using the rheometers to measure the viscosity and elasticity. •Finding the intrinsic viscosity using capillary viscometers.
Dec 23, 2015
Objectives To determine the properties and
quality of the horse joint supplements Legend and Hyalovet by:
•Using the rheometers to measure the viscosity and elasticity.
•Finding the intrinsic viscosity using capillary viscometers.
Rheometers
Rate Control Strain Control
Viscometer Glass tubes that
can be used to calculate the intrinsic viscosity of polymer solutions by measuring the rate at which a liquid falls through the capillary at different dilutions.
Capillary
Timing Lines
Hyaluronic Acid
•Biopolyelectrolyte•High molecular weight•viscoelastic properties•costs 100,000 per kilogram
Sources of HA•rooster combs•micro-organisms•umbilical cords
Intrinsic Viscosities of HA Supplements in PBS
0.00
200.00
400.00
600.00
800.00
1000.00
1200.00
1400.00
1600.00
1800.00
2000.00
0.00E+00 2.00E-04 4.00E-04 6.00E-04 8.00E-04 1.00E-03 1.20E-03
Concentration HA (mg/ml)
(T-T
S)/
(TS
*C)
Trial 1 Hyalovet
Trial 2 Hyalovet
Trial 1 Legend
Trial 2 Legend
Linear (Trial 2Hyalovet)Linear (Trial 1Hyalovet)Linear (Trial 2Legend)Linear (Trial 1Legend)
IV = 534IV = 531(ml/g)
Intrinsic Viscosity = 1074 ml/g
IV = 991 ml/g
Moduli of Legend and Hyalovet
0.00
0.01
0.10
1.00
10.00
0.10 1.00 10.00
Frequency (hz)
Mod
uli (
Pa) Legend Elastic Modulus (Pa)
Legend Viscous Modulus (Pa)
Hyalovet Elastic Modulus (Pa)
Hyalovet Viscous Modulus (Pa)
Viscosity of Legend and Hyalovet
0.01
0.10
1.00
0.10 1.00 10.00 100.00 1000.00
Shear Rate (1/s)
Vis
cosi
ty (
Pas
)
Legend Viscosity
Hyalovet Viscosity
They Came to Tame the Lame
Conclusions The experiments performed on the
rheometer showed similar readings for the flow of Legend and Hyalovet
Legend and Hyalovet showed the same viscous and elastic moduli.
The intrinsic viscosity of Hyalovet is twice that of Legend which shows that Hyalovet has a larger molecular weight.