November Update

November 2012

In this issue…

• PeproGrow-ESC

• R-Spondin Proteins

• Human EGFL7

• Human C1inh

• Human IL-36RA

• Human ANGPTL-3

• Human ANGPTL-6

• Special Promotions

www.peprotech.com

®

PeproGrow-ESC Complete Stem Cell MediaPeproTech now introduces PeproGrow-ESC Stem Cell Media. PeproGrow-ESC is a complete, serumfree, chemically defined formulation medium for feeder-free culture systems for maintenance andexpansion of human embryonic and induced pluripotent stem cells (ESCs) in the undifferentiatedstate (SSEA4+/Oct4+) using BD Matrigel® as surface coating. The proprietary formulation containsrelevant growth factors including FGF2, but no Insulin. The complete PeproGrow-ESC is a novel formulation that was developed and designed by PeproTech in collaboration with Rutgers Stem CellTraining Course. It represents the best currently available media for supporting the proliferation ofstem cells in an undifferentiated state.

Catalog Number: BM-ESC PeproGrow-ESC

Catalog Number: AF-BM-ESC Animal Free PeproGrow-ESC

R-Spondin ProteinsThe R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consistsof four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondinstructural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in amanner that causes the stabilization and resulting accumulation of the intracellular signaling protein,β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype,and can also regulate the proliferation and differentiation of certain stem cell populations.

Recombinant CHO cell derived R-Spondins are glycoproteins containing between 222 and 251amino acid residues.

Catalog Number: 120-38 R-Spondin-1

Catalog Number: 120-43 R-Spondin-2

Catalog Number: 120-44 R-Spondin-3

NOVEMBER 2012 • PEPROTECH PRODUCT UPDATE

AvailableSoon!

ProductUpdate

Special Discounts Available on Bulk Products!Bulk products available at

special discount prices.

Contact [email protected]

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Manufacturer

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Cytokine Products

Human EGFL7 (Epidermal Growth Factor Like Protein 7)EGFL7 (Epidermal Growth Factor Like Protein 7, Multiple EGF Like Domains Protein 7, VE Statin) isa multi-domain protein containing two EGF like domains and one EMI domain. It is expressed almostexclusively in endothelial cells and functions to promote normal development of the vascular system,particularly tubulogenesis. EGFL7 is capable of antagonistic binding to Notch receptors resulting inthe inhibition of Notch signaling in HUVEC and neural stem cells. In research models inducing hypoxia and subsequent reoxygenation, (H/R), EGFL7 can inhibit ICAM-1 expression and enhancethe inhibition of NF-kappaB activation. Additionally, EGFL7 can chemoattract endothelial cells andbind to the extracellular matrix. The overexpression of EGFL7 is observed in various cancers, and isgenerally correlated with increased metastasis and a poor prognosis.

Recombinant E. Coli derived EGFL7 is a 28 kDa protein containing 251 amino acid residues.

Catalog Number: 100-61 Human EGFL7

Human ANGPTL-3, ANGPTL-6ANGPTL-3 (Angiopoietin like protein 3) and ANGPTL-6 (Angiopoietin like protein 6) are membersof the angiopoietin family of structurally related proteins, characterized by a coiled N-terminal domain and a C-terminal fibrinogen like domain. These proteins are primarily expressed in the liver and can exert activities related to both angiogenesis and metabolism. ANGPTL-3 inhibitslipoprotein lipase (LPL) and endothelial lipase (EL), which has the effect of increasing plasma levelsof triglycerides and HDL associated cholesterol. The fibrinogen like portion of the ANGPTL-3 proteincan bind alpha-5/beta-3 integrins leading to endothelial cell adhesion and migration. ANGPTL-6participates in regulating glucose homeostasis by inhibiting transcriptional activity of Fox01 and reducing the production of glucose-6 phosphatase (G6Pase). ANGPTL-6 can also chemo-attract endothelial cells as part of its angiogenic related activity.

Recombinant CHO cell derived ANGPTL-3 is a glycoprotein containing 449 amino acid residues.Recombinant CHO cell derived ANGPTL-6 is a glycoprotein containing 450 amino acid residues.

Catalog Number: 130-18 Human ANGPTL-3

Catalog Number: 130-21 Human ANGPTL-6

PEPROTECH PRODUCT UPDATE • NOVEMBER 2012

NOVEMBER 2012 • PEPROTECH PRODUCT UPDATE

Human C1inh (C1 inhibitor)C1 Inhibitor (C1inh, C1 Esterase Protein, Serpin G1) is a member of the serpin family of structurally related proteins, and is theprimary regulator of the immune complement system. C1inh is a protease inhibitor that functions to inhibit the complementsystem in order to prevent over-activation or spontaneous activation. Inhibition is achieved by binding to and irreversibly inhibiting the C1r and C1s proteases of the C1 complex, which has the effect of shutting down all subsequent downstreamevents in the complement activation cascade. C1inh can also inhibit various other proteases, including Kallikrein, Factor XIa,and Factor XIIa. Deficiencies in C1inh are the primary cause of hereditary angioedema (HAE, hereditary angioneurotic edema),a disease characterized by edema in the respiratory and gastrointestinal tracts. In certain clinical situations, the direct administration of C1inh can be used to treat HAE and certain other conditions.

Recombinant CHO cell derived C1inh is a highly glycosylated glycoprotein containing 488 amino acid residues, and is fullyfunctional in its ability to inhibit the C1 complex.

Catalog Number: 130-20 Human C1inh

Human IL-36RAThe IL-1 family is comprised of 11 structurally related ligands, including the recently re-named IL-36RA (IL-1F5), IL-36α (IL-1F6),IL-36β (IL-1F8), and IL-36γ (IL-1F9). The interaction of IL-36 ligands with the IL-1Rrp2 receptor (IL-1R6) can induce various activities, including dendritic cell maturation and activation. IL-36RA can antagonize the NF-kappaB signaling induced by eitherIL-36α, -β or -γ by binding to the IL-1Rrp2 receptor in a manner that prevents the initiation of functional signaling.

Recombinant IL-36RA is an E. Coli derived 17 kDa protein containing 154 amino acid residues.

Catalog Number: 200-36RA Human IL-36RA

Take Advantage of our 3 for the Price of 2 Deal by December 31, 2012!*Buy any two Size A or B vials of Proteins or Antibodies and receive a third vial of equal or lesser value for free now through December 31, 2012. All Size A & B vials can be found in our current catalog and on our website at www.peprotech.com. To request our current catalog, contact [email protected].

* Excludes Animal-Free Products, ELISA Development Kits, Cytokine Packages and Media Supplements. Offer valid to End-User Clients only. Distributors, wholesale purchasers and any other non-End-User Clients are not eligible for this discount. Offer cannot be combined with any other pricingdiscounts/contracts/quotations. Offer discounts are not reflected in prices or totals provided online. Discounts will be applied to final invoice pricing.

PRODUCT UPDATE

is intended to update

our customers about

products and services

offered by PeproTech.

PeproTech Inc.

5 Crescent Avenue

P.O. Box 275

Rocky Hill, New Jersey

08553-0275

Phone: (800) 436-9910

Fax: (609) 497-0321

[email protected]

www.peprotech.com

PEPROTECH PRODUCT UPDATE • NOVEMBER 2012

We invite you to visit our New & Improved Website: www.peprotech.com

• MSDS & Lot-Specific Product Data Sheet Search

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PeproTech Inc.5 Crescent Avenue • P.O. Box 275Rocky Hill, New Jersey 08553-0275 • USA

New Products

Proteins Catalog #

Human BMP-10 120-40

Human LIF 300-05

Human Uteroglobin 150-18

Human Vimentin 110-10

Murine LIF 250-02

Animal-Free Proteins

Human IFN-λ1 AF-300-02L

Human IL-8 (78 a.a.) (CXCL8) AF-200-08M

Human IL-17E AF-200-24

Human IL-33 AF-200-33

Human IL-13 AF-200-13

Human LIF AF-300-05

Human SDF-1α (CXCL12) AF-300-28A

Human SDF-1β (CXCL12) AF-300-28B

Human TPO AF-315-14

Polyclonal Antibodies Catalog #

Biotinylated Anti-Rat IL-1α 500-P180GBt

Mini-ELISA

Development Kits

Human sRANK-Ligand 900-M142

Murine IL-21 900-M368

Murine GM-CSF 900-M55

Murine VEGF 900-M99

Rat MCP-1 900-M59

Rat IP-10 900-M449

Cell Culture

Media Products

CHO Media CM-CHO-S