-
Novel regulatory mechanisms underlying the expression of the
carbohydrate
response element binding protein (ChREBP): the roles of insulin
and the POU
protein Oct-1
by
Adam Sebastian Sirek
A thesis submitted in conformity with the requirements
for the degree of Master of Science
Department of Physiology
University of Toronto
© Copyright by Adam Sebastian Sirek (2009)
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ii
Novel regulatory mechanisms underlying the expression of the
carbohydrate response element binding protein (ChREBP): the roles
of insulin and the POU protein Oct-1
Master of Science, 2009 Adam Sebastian Sirek
Department of Physiology University of Toronto
ABSTRACT
ChREBP has emerged as one of the key controllers of hepatic
lipogenesis. While the
function of ChREBP has been extensively investigated, mechanisms
underlying its
transcriptional regulation remain largely unknown. We located a
conserved POU-binding
site within mammalian ChREBP promoters, and demonstrated that
the POU homeodomain
protein Oct-1 binds to this site in the human HepG2 cell line.
Oct-1 transfection significantly
repressed ChREBP promoter activity 50-75%. Conversely, knockdown
of Oct-1 expression
with shRNA significantly increased ChREBP expression levels.
Furthermore, insulin
treatment resulted in a two-fold activation of ChREBP promoter
activity, and stimulated
endogenous ChREBP expression. We found that the stimulatory
effect of insulin on the
ChREBP promoter is at least partially dependent on the presence
of the POU-binding site,
and that insulin treatment reduced Oct-1 expression. Our
observations identify Oct-1 as a
transcriptional repressor of ChREBP, and suggest that insulin
stimulates ChREBP expression
via attenuating the repressive effect of Oct-1.
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ACKNOWLEDGMENTS
I would like to thank my two supervisors, Dr. Dominic Ng and Dr.
Tianru Jin, whose
knowledge and suggestions made possible the inception,
execution, and completion of this
project. My committee members, Dr. Khosrow Adeli and Dr. Allen
Volchuk have been
equally helpful and provided me with a solid basis on which to
critically review and reassess
my work. For all of this, I should like to express my deepest
gratitude.
I would also like to thank all of my colleagues and the members
of the Jin and Ng labs. In
particular, I would like to mention Dr. Ling Liu for her
preliminary work on the project. In
addition, I would like to thank Janie Sun and Sarah Khalid for
the countless ideas and
guidance, and of course, humour and companionship, throughout my
entire project. Were
it not for them, the time spent would have been considerably
more dull and difficult.
During my undergraduate years, my grandmother, Dr. Anna Sirek,
introduced me to Dr.
Mladen Vranic, and ever since, has been the first and most
patient critic of all my work. Dr.
Vranic provided me with my first opportunity to be involved in
medical research and has
been an exceptionally kind and generous mentor ever since my
first summer in his lab.
Without Dr. Vranic and his encouragements, I would likely have
not arrived where I am
today. I find it almost impossible to express my gratitude
toward him in proper words.
Finally, I would like to thank my wife, Danielle for all the
understanding, support, and
encouragement she has offered.
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TABLE OF CONTENTS
Abstract
.....................................................................................................................................
ii
Acknowledgments
....................................................................................................................
iii
Table of Contents
.....................................................................................................................
iv
List of
Figures............................................................................................................................
ix
List of Tables
............................................................................................................................
xii
List of Abbreviations
...............................................................................................................
xiii
List of Publications and Presentations
......................................................................................
xv
Chapter 1. Introduction
................................................................................................1
1.01 Regulation of Gene Expression
....................................................................1
1.02 General Overview of Hepatic Carbohydrate Metabolism
.............................4
1.02.1 Metabolism of Dietary Carbohydrates
.........................................................6
1.02.2 Insulin Signalling
..........................................................................................6
1.02.3 Pathophysiology of Metabolic Disorders
.....................................................9
1.03 The Carbohydrate Response Element Binding Protein
(ChREBP)................12
1.03.1 Overview
...................................................................................................12
1.03.2 Regulation of ChREBP activity by post-translational
modifications:
Phosphorylation and De-phosphorylation
.................................................14
1.03.3 Role of ChREBP in Glucose Metabolism
.....................................................16
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1.03.4 Transcriptional Regulation of ChREBP
........................................................18
1.04 The POU Homeodomain Protein Oct-1
......................................................20
1.04.1 POU Domain Superfamily
..........................................................................20
1.04.2 The Octamer Binding Proteins
...................................................................22
1.04.3 The Function of Oct-1
................................................................................24
1.04.4 Oct-1 and Metabolic
Stress........................................................................25
Chapter 2. Rationale and Hypothesis
..........................................................................27
2.01 Whether Oct-1 Functions as a Repressor of ChREBP Expression
................28
2.02 Whether Insulin Treatment Stimulates ChREBP Expression
.......................29
2.03 Whether Insulin Stimulates ChREBP Expression via
Attenuating the Effect of
Oct-1 on the ChREBP Promoter
.................................................................29
Chapter 3. Materials and Methods
.............................................................................32
3.01 Chemicals
..................................................................................................32
3.02 Plasmid Construction
................................................................................32
3.03 Cell
Culture................................................................................................33
3.04 DNA Transfection and Luciferase Reporters
..............................................34
3.05 Lentiviral shRNA Knockdown
.....................................................................34
3.06 RNA Extraction and Real Time RT-PCR
.......................................................35
3.07 Chromatin Immunoprecipitation
...............................................................35
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3.08 Nuclear Protein Extraction
........................................................................37
3.09 Immunoblotting
........................................................................................37
3.10 Electrophoretic Mobility Gel Shift Assay
(EMSA)........................................38
3.11 Statistical Analysis
.....................................................................................39
Chapter 4. Results
......................................................................................................40
4.01 Oct-1 as a negative transcriptional repressor of ChREBP
expression ..........40
4.01.1 Oct-1 interacts with the POU protein binding site located
within the
proximal promoter region of ChREBP
........................................................40
4.01.2 Oct-1 functions as a transcriptional repressor of ChREBP
..........................43
4.01.3 Lentiviral mediated knockdown of Oct-1 results in
increased ChREBP
expression
.................................................................................................46
4.01.4 Oct-1 over-expression results in decreased ChREBP
expression.................50
4.02 Insulin treatment leads to increased ChREBP expression
...........................52
4.02.1 Insulin stimulates ChREBP promoter activity
.............................................52
4.02.2 Insulin stimulates ChREBP protein expression
...........................................55
4.02.3 The effect of insulin on ChREBP expression in HepG2 cells
that express Oct-
1
shRNA.....................................................................................................55
4.02.4 The effect of insulin on ChREBP expression in HepG2 cells
over-expressing
Oct-1
.........................................................................................................59
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4.02.5 Insulin treatment increases ChREBP expression in primary
hamster
hepatocytes...............................................................................................63
4.02.6 L-PK mRNA expression can be altered by Oct-1 shRNA and
insulin
treatment
..................................................................................................65
4.03 Insulin stimulation of ChREBP expression is dependent on
the POU protein
binding site located in the proximal promoter region of the
ChREBP
promoter
...................................................................................................65
4.03.1 The repressive effect of Oct-1 on ChREBP expression is
dependent on the
POU-binding site in the ChREBP proximal promoter
..................................65
4.03.2 The stimulatory effect of insulin on ChREBP expression is
at least partially
dependent on the POU-binding site in the ChREBP proximal
promoter .....66
4.03.3 Insulin treatment attenuates Oct-1 nuclear expression
levels ...................68
4.03.4 Insulin treatment reduces Oct-1 binding to the ChREBP
promoter in intact
cells
...........................................................................................................71
4.03.5 Inhibition of PI3K or ERK affects Oct-1 binding to the
ChREBP promoter
..................................................................................................................74
4.03.6 ERK, but not AKT plays a role in mediating the
stimulatory effect of insulin
on ChREBP promoter activity
.....................................................................77
4.03.7 MEK inhibition attenuates the stimulatory effect of
insulin on ChREBP
mRNA expression
......................................................................................77
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Chapter 5. Discussion
.................................................................................................81
5.01 Oct-1 represses ChREBP expression, and this repression
relies on the POU-
binding site conserved within the ChREBP promoter
.................................81
5.02 Insulin treatment stimulates ChREBP expression
.......................................83
5.03 Insulin stimulates ChREBP expression via attenuating the
repressive effect
of Oct-1 on the ChREBP promoter
.............................................................85
Chapter 6. Summary and
Conclusions.........................................................................89
Chapter 7. Future
Directions.......................................................................................92
7.01 Whether Oct-1 is phosphorylated in response to the
activation of insulin
signalling?
.................................................................................................92
7.02 Whether, after insulin, Oct-1 is excluded from the nucleus,
degraded, or
both?
........................................................................................................92
7.03 What is the effect of Oct-1 on the ChREBP promoter in vivo?
....................93
Chapter 8. References
................................................................................................95
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LIST OF FIGURES
Figure 1: An illustration of the interactions of ChREBP and
SREBP-1c with lipogenic enzyme gene
promoters.
......................................................................................................................7
Figure 2: An illustration of the Insulin Signalling Pathway
............................................................8
Figure 3: An illustration of the major domains in ChREBP
..........................................................13
Figure 4: Mechanisms underlying ChREBP activation/inactivation
.............................................15
Figure 5: The role of ChREBP in metabolic regulation
................................................................17
Figure 6: The overall domain structure of Oct-1
........................................................................21
Figure 7: An illustration of the hypotheses of this study
............................................................31
Figure 8: The POU-binding site is highly conserved within ChREBP
promoters...........................41
Figure 9: Oct-1 binds to the POU-binding site located within the
ChREBP promoter..................42
Figure 10: Oct-1 acts as a repressor of the human ChREBP
promoter constructs in Cos-7
fibroblasts
.....................................................................................................................44
Figure 11: Oct-1 acts as a repressor of ChREBP promoter activity
in the HepG2 cell line ...........45
Figure 12: Transient transfection of Oct-1 represses ChREBP
protein expression ......................47
Figure 13: Generation of HepG2 cells stably expressing Oct-1
shRNA ........................................48
Figure 14: Oct-1 shRNA expressing cells demonstrate increased
ChREBP expression ................49
Figure 15: Oct-1 over-expressing cells demonstrate decreased
ChREBP expression ..................51
Figure 16: Insulin treatment increases ChREBP promoter activity
.............................................53
Figure 17: Insulin treatment increases ChREBP protein expression
...........................................54
Figure 18: Insulin treatment (10nM) increases ChREBP promoter
activity in HepG2 cells
expressing Oct-1 shRNA
.................................................................................................56
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Figure 19: Insulin treatment (100nM) increases ChREBP promoter
activity in HepG2 cells
expressing Oct-1 shRNA
.................................................................................................57
Figure 20: Insulin treatment (10nM) increases ChREBP mRNA and
protein levels in HepG2 cells
expressing Oct-1 shRNA
.................................................................................................58
Figure 21: Insulin treatment (10nM) increases ChREBP promoter
activity in HepG2 cells over-
expressing Oct-1
............................................................................................................60
Figure 22: Insulin treatment (100nM) increases ChREBP promoter
activity in HepG2 cells over-
expressing Oct-1
............................................................................................................61
Figure 23: Insulin treatment (10nM) increases ChREBP mRNA and
protein levels in the HepG2
cells over-expressing Oct-1
............................................................................................62
Figure 24: Insulin treatment increases ChREBP and lipogenic
target expression levels ..............64
Figure 25: The repression of ChREBP promoter activity by Oct-1
is dependent on the POU
binding site
....................................................................................................................67
Figure 26: Insulin stimulation of HepG2 cells treated with Oct-1
shRNA is dependent on the POU
binding site
....................................................................................................................69
Figure 27: Insulin stimulation of HepG2 cells over-expressing
Oct-1 is dependent on the POU-
binding site
....................................................................................................................70
Figure 28: Insulin treatment reduces Oct-1 nuclear content
......................................................72
Figure 29: Insulin treatment reduces Oct-1 binding to the ChREBP
Promoter in vivo .................73
Figure 30: Insulin stimulation leads to reduced Oct-1 content
...................................................75
Figure 31: Reduced binding of Oct-1 to the ChREBP promoter
following insulin treatment can be
blocked with ERK inhibition
...........................................................................................76
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Figure 32: Insulin stimulation of ChREBP promoter activity can
be blocked with MEK/ERK
inhibition
.......................................................................................................................78
Figure 33: The stimulatory effect of insulin on ChREBP mRNA
expression can be blocked with
MEK/ERK
inhibition........................................................................................................79
Figure 34: Proposed mechanisms underlying the stimulation of
insulin on ChREBP expression .91
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LIST OF TABLES
Table 1: An overview of the various identified forms of POU
homeodomain proteins ...23
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LIST OF ABBREVIATIONS
ACC Acetyl-Co A Carboxylase ACL ATP-Citrate Lysase AKT
Serine/Threonine Specific Protein Kinase B AMP Adenosine
Monophosphate AMPK AMP-Activated Protein Kinase bHLH Basic
Helix-Loop-Helix Domain bHLH/ZIP Basic Helix-Loop-Helix
Leucine-Zipper Domain BSA Bovine Serum Albumin bp Base Pairs C/EBP
CAAT Enhancer-Binding Protein cAMP Cyclic Adenosine Monophosphate
CBP CREB Binding Protein ChIP Chromatin Immunoprecipitation ChoRE
Carbohydrate Response Element ChREBP Carbohydrate Response Element
Binding Protein CoA Coenzyme A DNA Deoxyribunucleic Acid EMSA
Electrophoretic Mobility Shift Assay ERK Extracellular Signalling
Regulated Kinase FA Fatty Acid FAS Fatty Acid Synthase FBS Fetal
Bovine Serum Fru-6-P Fructose 6 Phosphate GAP Glyceraldehyde 3
Phosphate GFP Green Flourescence Protein Glu-6-P Glucose 6
Phosphate GSK3 Glycogen Synthase Kinase 3 GWA Genome-Wide
Association H2B Histone H2B HD Homeodomain HDAC Histone Deacetylase
1 HDL High Density Lipoprotein HSV Herpes Simplex Virus HSV IE
Herpes Simplex Virus Immediately Expressed Genes HTG
Hypertriglyceridemia IR Insulin Receptor IRS Insulin Receptor
Substrate kb Kilobase Pairs kDa Kilodalton LDL Low Density
Lipoprotein L-PK L-Type Pyruvate Kinase LUC Luciferase LXR Liver X
Receptor MAPK Mitogen Activated Protein Kinase ME Malic Enzyme MEK
MAPK/ERK Kinase
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Mlx Max-Like Protein X MLXIPL Max-Like Protein X Interacting
Protein MUFA Mono-Unsaturated Fatty Acid NAFLD Non-Alcoholic Fatty
Liver Disease NES Nuclear Entry Signal NF-Y Nuclear Transcription
Factor Y NLS Nuclear Localization Signal Oct Octamer Binding
Transcription Factor Oct-1 Octamer Binding Transcription Factor 1
Oct-2 Octamer Binding Transcription Factor 2 oxLDL Oxidized Low
Density Lipoprotein PAK-1 Protein Activated Kinase 1 PCR Polymerase
Chain Reaction PEP Phosphoenolpyruvate PI3K Phosphoinositide Kinase
3 Pit-1 Pituitary Specific Transcription Factor 1 PKA Protein
Kinase A pol RNA Polymerase POU Super family of transcription
factors containing a bipartite DNA binding domain POU domain A
bipartite DNA binding domain characteristic of members of the POU
family POU2F1 Oct-1 synonym PP Protein Phosphatase PP2a Protein
Phosphatase 2a PPARγ Peroxisome Proliferator-Activated Receptor
Isoform Gamma Pro-Rich Proline Rich Region PUFA Polyunsaturated
Fatty Acid RNA Ribonucleic Acid SCD-1 Sterol Co-Enzyme Desaturase 1
SDS Sodium Dodecyl Sulphate shRNA Small Hairpin RNA SMRT Silencing
Mediator of Retanoic Acid and Thyroid Hormone Receptor snRNA Small
Nuclear RNA Sp1 A Human Transcription Factor Involved in Early
Development SRE Sterol Response Element SREBP-1c Sterol Response
Element Binding Protein Isoform 1-c T2DM Type 2 Diabetes Mellitus
TBP TATA Box Binding Protein TCA Citric Acid Cycle Tg Triglyceride
Unc-86 Neural Transcription Factor 86 (C. elegans) Xu-5-P
Xylulose-5-Phosphate ZIP-Like A Zip-like domain
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LIST OF PUBLICATIONS AND PRESENTATIONS
(i) Project Specific Publications
Sirek, AS., Liu, L., Naples, M., Adeli, K., Ng, DS., Jin, T.
Insulin stimulates the expression of Carbohydrate Response Element
Binding Protein (ChREBP) by attenuating the repressive effect of
POU homeodomain protein Oct-1. EPub ahead of print, Endocrinology,
April 9th, 2009.
(ii) Project Specific Oral Presentations
Sirek, AS., Ng, DS., and Jin, T. Insulin stimulates the
expression of the Carbohydrate Response Element Binding Protein
(ChREBP) by attenuating the effect of the POU homeodomain protein
Oct-1. Banting and Best Diabetes Centre and Samuel Lunenfeld
Research Institute Diabetes Seminar Series, University Health
Network, Toronto, ON, March 2009.
Sirek, AS., Ng, DS., and Jin, T. Transcriptional Regulation of
ChREBP: Controlling a Key Player in Lipogenesis. Canadian Diabetes
Association Professional Conference and Annual Meetings, Montreal,
QC, October 2008.
Sirek, AS., Ng, DS., and Jin, T. Transcriptional Regulation of
the Carbohydrate Response Element Binding Protein (ChREBP): A New
Look at a Lipogenic Control. Diabetes in Motion Speaker Series,
Waterloo, ON, October, 2008.
Sirek, AS., Ng, DS., and Jin, T. The POU homeodomain protein
Oct-1 as a potential transcriptional repressor of ChREBP, a key
regulator of lipogenesis. Diabetes Discovery Research Group,
University Health Network, Toronto, ON, May 2008
(iii) Project Specific Poster Presentations
Sirek, AS., Liu, L., Ng, DS., Jin, T. A novel mechanism in
controlling the expression of ChREBP: The involvement of insulin
and the POU homeodomain protein Oct-1. American Diabetes
Association 69th Scientific Sessions, New Orleans, LA, U.S.A., June
2009.
Sirek, AS., Liu, L., Ng, DS., Jin, T. Mechanisms controlling the
expression of of the Carbohydrate Response Element Binding Protein
(ChREBP): Insulin, and Oct-1. Banting and Best Diabetes Centre
Annual Research Day, Toronto, ON, May 2009.
Sirek, AS., Liu, L., Ng, DS., Jin, T. A novel mechanism in
controlling the expression of ChREBP. Endocrinology and Diabetes
Research Group 2nd Annual Research Day, University of Toronto,
Toronto, ON, May 2009.
Sirek, AS., Ng, DS., and Jin, T. Transcriptional Control of the
Carbohydrate Response Element Binding Protein (ChREBP).
Endocrinology and Diabetes Research Group 1st Annual Research Day,
Toronto, ON, May 2008.
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Chapter 1. INTRODUCTION
1.01 Regulation of Gene Expression
Eukaryotic gene expression can be regulated at multiple steps,
ultimately affecting the
conversion of information stored in DNA into functional
proteins. The differences in cell
types depend on the particular genes that are expressed.
Additionally, as demonstrated in
various cancers, superfluous expression of genes at incorrect
times may lead to devastating
pathophysiological conditions. Therefore, control mechanisms in
gene expression are
important to understand. The major steps in gene expression
where control mechanisms
are often found include:
1. Transcriptional Control – controlling when and how often a
given gene is
transcribed,
2. RNA Processing Control – controlling how the mRNA transcript
is spliced or
otherwise processed,
3. RNA Transport and Localization Control – controlling which
completed
mRNAs in the cell nucleus are exported into the cytosol and
determining
where in the cytosol they are localized,
4. Translational Control – controlling which mRNAs in the
cytoplasm are
translated by ribosomes,
5. mRNA Degradation Control – controlling destabilization of
certain mRNA
molecules in the cytoplasm,
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6. Post Translational Modification – the chemical and enzymatic
modification of
proteins following their translation, and
7. Protein Activity Control – controlling the selective
activation, inactivation,
degradation, or compartmentalization of specific protein
molecules after
they have been made.
These mechanisms controlling gene expression are important in
the determination of
cell fate. There is only one mechanism, transcriptional control,
which ensures that the cell
will not synthesize superfluous intermediates. This is the
mechanism of regulation which we
chose to study in detail, in order to identify mechanisms
underlying ChREBP gene
expression.
Gene transcription is controlled by a regulatory region of DNA
close to the site in which
transcription begins, termed the “promoter”. There are many
variations on this regulatory
region, some are simple switches, which turn on or off gene
expression; while others are
more complex, and act to integrate various signals present in
the nucleus in order to
determine whether a gene should be expressed at a certain time.
Both forms of promoters,
simple and complex, contain two fundamental components: Short
stretches of DNA which
contain defined and conserved sequences; and gene regulatory
proteins which recognize
and bind to these defined sequences.
Typically, nucleotide sequences shorter than 20 bp function as
the fundamental
components for specific gene regulatory protein binding. Studies
have identified thousands
of these sequences and their interacting proteins. Generally,
molecular recognition requires
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an exact fit between the surfaces of two molecules in order to
allow their interaction (1).
This same principle applies in DNA-protein interactions. Gene
regulatory proteins, or
transcription factors, recognize a specific DNA sequence because
the surface of the protein
is complementary to the special surface features present in the
DNA double-helix.
Generally, these proteins make interactions which include
hydrogen-bonds, ionic bonds,
and hydrophobic interactions (2). While individually, these
interactions may be weak,
upwards of 20 of these interactions may act together to allow
stronger affinity (2, 3).
Interestingly, studies of DNA-protein recognition often fall
into various categories of
interaction based upon the motifs found in the protein. These
motifs often use α-helices or
β-sheets to bind to the major groove of DNA, as this groove
often contains sufficient
information to distinguish various DNA sequences.
Eukaryotes use gene regulatory proteins to activate or repress
the expression of genes.
These proteins bind to the promoter or regulatory element such
as enhancers of the gene,
which may be thousands of nucleotides distant from the gene
itself. Since this region is so
large, it has been termed the gene control region. It is
generally accepted to be made up of
a promoter (where the general transcription factors and
polymerase assemble), and
regulatory sequences (to which regulatory proteins bind to
control the rate of assembly at
the promoter). The distance between these regions may be
considered flexible space
needed for communication, and interactions between DNA-bound
proteins.
There are a small number of “general transcription factors”
which are abundant
proteins required to initiate the assembly of a complex which
recruits RNA polymerase II to
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a gene promoter. The gene regulatory transcription factors are
considerably more
numerous and allow for great specificity in control mechanisms.
Different selections of gene
regulatory proteins are present in different cell types and thus
different patterns of gene
expression are generated which give that cell its particular
characteristics. Due to this, the
understanding of transcription factors expressed in a particular
cell type can divulge a great
deal of information regarding processes specific to that tissue
and any pathologies which
may arise therein.
1.02 General Overview of Hepatic Carbohydrate Metabolism
The liver is the main site at which nutrients, which have been
absorbed from the gut and
then transferred into the blood, are processed for use by other
cells in the body. The liver is
a large organ which develops at a site in the body where a major
vein runs close to the wall
of the primitive gut. Thus, the adult organ retains a close
relationship with the circulatory
system. In fact, the majority of the liver’s blood is supplied
directly from the intestinal tract
via the portal vein. Cells in the liver that derive from
primitive gut epithelium (hepatocytes)
are arranged in interconnected sheets and chords with
blood-filled spaces running in
between them. The single layer of flattened endothelial cells
that covers the exposed
surfaces of the hepatocytes allows for one of the chief
functions of the liver: the exchange
of metabolites between hepatocytes and the blood.
Hepatocytes are required for the synthesis, degradation, and
storage of a large variety
of substances. They also play a large role in the carbohydrate
and lipid metabolism of the
entire body, and secrete a large quantity of the protein found
in blood plasma. In fact, the
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primary site for the conversion of excess dietary carbohydrates
into triglycerides (Tg) is
within the liver. An increase in dietary carbohydrates leads to
increased expression of
several key regulatory enzymes that are involved in lipogenesis,
including L-Type Pyruvate
Kinase (L-PK), Acetyl Co-A Carboxylase (ACC), Malic Enzyme (ME),
ATP-Citrate Lysase (ACL),
and Fatty Acid Synthase (FAS).
Until recently, insulin was believed to be the main inducer of
lipogenic gene expression,
mediated via the Sterol Regulatory Element Binding Protein 1-c
(SREBP-1c) (4). This
transcription factor, a member of the basic
helix-loop-helix-leucine-zipper (bHLH/ZIP)
family, induces lipogenic gene expression through binding to the
sterol regulator element
(SRE) within the promoter sequence of its target genes (5-7).
SREBP-1c is also potently
regulated by liver X receptors (LXR) which heterodimerize with
retinoid X receptors (RXR)
(8-10) and bind to a sequence known as the LXR response element.
Studies in SREBP-1c-/-
mice, however, demonstrated that there is only about 50%
reduction in fatty acid synthesis
(11). Thus, SREBP-1c alone cannot account for all lipogenic
activity in response to glucose
and insulin signalling.
In 2001, the carbohydrate response element binding protein
(ChREBP) was purified and
identified as the long-sought carbohydrate response element of
the cell (12). This
transcription factor, which responds to glucose, up-regulates a
similar panel of downstream
target genes as SREBP-1c, including L-PK, ACC, ME, ACL, and FAS.
The involvement of SREBP-
1c and ChREBP in controlling the activation of lipogenic target
genes is illustrated in Figure
1.
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6
1.02.1 Metabolism of Dietary Carbohydrates
The dietary carbohydrates which we ingest must be broken down
into simple sugars for
use by the cells as energy sources or as building blocks for
synthesizing a variety of
molecules. Though predominantly active in the liver, additional
sites of lipogenesis include
adipose tissue, and the mammary glands. Lipogenesis involves the
conversion of
carbohydrates such as glucose, into fatty acids. Fatty acids are
then used for synthesis of
phospholipids, esterified cholesterol, and triglycerides. Fatty
acids from lipogenesis and the
circulating pool both contribute significantly to the
triglyceride pool that are destined for
packaging into lipoproteins such as the very-low density
lipoprotein (VLDL) and secreted
from the liver. Synthesis of fatty acids occurs in the cytosol
of the cell and generally begins
with the molecule Acetyl-CoA. The oxidation and breakdown of
fatty acids occurs within the
mitochondria. Many of the enzymes involved in lipogenesis are
grouped into a multienzyme
complex termed “Fatty Acid Synthase” (FAS).
1.02.2 Insulin Signalling
Important in activating and regulating some of the metabolic
pathways involved in
hepatic lipogenesis is the insulin signalling pathway. Insulin,
discovered in 1921, provided
some of the first evidence for the activity of endocrine
secretions. Insulin was demonstrated
to be the key hormone which allowed the entry of glucose from
the bloodstream into the
cell, thus lowering glycemia, and in many cases, providing
effective therapy for diabetes.
Extensive studies on this important hormone have demonstrated
that it activates a
complicated signalling pathway with a large number of
targets.
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7
Figure 1: An illustration of the interactions of ChREBP and
SREBP-1c with lipogenic enzyme gene promoters.
The expression of genes involved in lipogenesis may be
controlled via two important transcription factors,
ChREBP (purple) and SREBP-1c (green). SREBP-1c has been well
characterized as an insulin-driven pathway,
while the more recent ChREBP has been linked to a
carbohydrate-induced lipogenic enzyme activation
pathway. Both transcription factors bind to specific sites in
the promoter region of the target gene (ChREBP to
ChoRE and SREBP-1c to SRE) to modulate gene expression. Negative
regulatory partners are indicated in red.
(Adapted from Towle, PNAS, 2001)
ChoRE SRE
ChREBP SREBP-1c
InsulinGlucose
Glucagon (cAMP)
PUFAs
Lipogenic Enzyme Expression
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8
Figure 2: An illustration of the Insulin Signalling Pathway
Insulin, binds to IR. IRS activates signalling cascade of
events. Insulin signalling is known to act through
two major pathways, via GSK3 and MEK/ERK. IR: Insulin Receptor;
IRS: Insulin Receptor Substrate; PI3K:
Phosphoinositide Kinase 3; AKT: Protein Kinase B; GLUT4; Insulin
Regulated Glucose Transporter 4; GSK3;
Glycogen Synthase Kinase 3; MEK: MAP Kinase/ ERK Kinase; ERK:
Extracellular Signalling Regulated Kinase.
(Image courtesy of Jane Sun)
IR
Insulin
Ras
IRSPI3K
Raf
MEK
ERK
Akt
PPP
P
GSK-3
Glycogen
Synthesis
Transcriptional Activation
PP2A
GLUT4 vesicle
GLUT4
Glucose
Apoptosis
Cytoplasm
Nucleus
Translation
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9
Insulin binds to the cell surface via the aptly named insulin
receptor. This in turn
activates a cascade of signalling events (13). Figure 2
summarizes some relevant elements
of this cascade for this study. These events include the
phosphorylation of both the
receptor and a number of members of the insulin receptor
substrate (IRS) family (14).
Tyrosine phosphorylation on IRS caused by insulin is a critical
event in mediating insulin
action and is generally impaired in cases of insulin resistance
(15, 16). Various pathways
may be activated in response to IRS phosphorylation
including:
1. The MAPK Pathway – which mediates various cellular effects
such as growth
and mitogenesis, and
2. The PI3K-Akt Pathway – which mediates gluconeogenesis,
glycogen and
protein synthesis in addition to being involved in GLUT4
translocation.
In insulin resistant states, the insulin signalling pathway is
dysregulated resulting in
inhibited glucose uptake.
1.02.3 Pathophysiology of Metabolic Disorders
Dysregulation of lipogenesis plays an important role in the
pathophysiology of many
metabolic diseases, including obesity, type 2 diabetes mellitus
(T2DM), and the metabolic
syndrome (17-19). Subjects with these disorders are known to be
at risk for a variety of
complications, including non-alcoholic fatty liver disease
(NAFLD), atherosclerosis, and
many cardiovascular complications (17, 20-23). Additionally,
subjects with T2DM and its
cardiovascular complications are often accompanied with
hyperinsulinemia,
-
10
hypertriglyceridemia (HTG), obesity, and insulin resistance.
Hence, there is increased
mortality in T2DM patients as a result of liver, cardiovascular,
and other complications (21,
24).
Diabetes mellitus is known to have two major forms, type 1
(T1DM), which is caused by
an autoimmune destruction of insulin producing β-cells of the
pancreas , and type 2, which
is caused by defects in insulin signalling and insulin
secretion. Over nutrition is commonly
accepted as a promoting factor for T2DM (25-27). As insulin
resistance progresses to
hyperglycemia, homeostatic mechanisms are activated to transform
these sugars into fats.
Thus, enhanced lipogenesis, development of obesity, and
hyperlipidemia frequently
precede the onset of type 2 diabetes.
Closely associated with T2DM is non-alcoholic fatty liver
disease (NAFLD). This condition
is emerging as one of the most common chronic liver diseases in
the Western hemisphere
(28), and contains a large spectrum of liver pathologies,
including steatosis, steatohepatitis,
cirrhosis, and hepatocellular carcinoma (5, 29-32). NAFLD has
been associated with obesity,
and insulin resistance (33-36), and this insulin resistance has
recently been identified as a
crucial patho-physiological factor in the development and
progression of NAFLD (37).
With the role of ChREBP elucidated in controlling lipogenic gene
expression, the
question of its role in the pathophysiology of hepatic steatosis
was raised. Triglycerides
(Tgs), synthesized from fatty acids, may derive their
bioconstituents from either plasma
nonesterified fatty acids, or fatty acids generated via de novo
lipogenesis. Excessive
accumulation of Tgs is one of the primary characteristics of
hepatic steatosis (38, 39).
-
11
Additionally, it is highly likely that ChREBP plays a large role
in the development of obesity
in cases of T2DM as the increased carbohydrates will lead to
ChREBP activation and
ultimately fat production. Interestingly, leptin-deficient
(ob/ob) mice show increased
hepatic lipogenesis and hepatic steatosis (40, 41). This had
previously been attributed to
SREBP-1c and PPARγ (42-44). However studies in SREBP-1c-/- and
PPARγ-/- mice showed only
partial improvement in the development of hepatic steatosis (23,
43). Recent studies have
implicated the involvement of ChREBP-mediated lipogenesis in the
development of fatty
liver (17, 40, 41).
It is well known that the number of patients displaying obesity
and elements of the
metabolic syndrome has reached epidemic proportions in both
Western (45-47) and
developing (48-52) countries. Due to these statistics, it has
become ever more important to
study the underlying metabolic control pathways which may
underlie these conditions.
Though SREBP-1c was first identified as an important
transcription factor in the control of
hepatic lipogenesis, it was not until later that ChREBP was
recognized as the carbohydrate
response element of the cell (23). The involvement of ChREBP in
a great number of
pathologies, including T2DM, obesity, NAFLD, and other elements
of the metabolic
syndrome, makes this transcription factor an important target
for future studies in order to
understand the underlying transcriptional control of hepatic
lipogenesis.
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12
1.03 The Carbohydrate Response Element Binding Protein
(ChREBP)
1.03.1 Overview
Studies in recent years have identified the Carbohydrate
Response Element Binding
Protein (ChREBP, also known as Mlx Interacting Protein Like,
MLXIPL) as another important
activator of lipogenic gene expression (12, 53-59). Due to this,
ChREBP is now understood to
be a key determinant of lipid synthesis in vivo, and as such,
has been intensively
investigated due to the possible implications for a variety of
metabolic disorders.
ChREBP was first identified in 2001 using affinity
chromatography and mass
spectrometry (53). The human gene that encodes ChREBP is located
on Chromosome 7. The
protein that was purified contained 852 amino acids and a
molecular weight of
approximately 95 kDa. This protein contains several regulatory
domains, including a nuclear
localization signal, a nuclear entry signal, a basic
helix-loop-helix-leucine-zipper (b/HLH/Zip),
and a leucine-zipper like (Zip-Like) domain (Figure 3). ChREBP
imparts transcriptional control
via binding to the carbohydrate response element (ChoRE) of its
lipogenic target gene
promoters (54, 60-65).
In primary cultures of hepatocytes, ChREBP was shown to induce
the activation of L-PK
following glucose treatment (12). The activation of L-PK and
other lipogenic enzymes lead to
the realization that this was the elusive “glucose-responsive
element” of the cell. ChREBP
does not homodimerize or bind to the ChoRE as a homodimer (54,
65). An analysis of
ChREBP using the yeast-two-hybrid system identified an
additional bHLH/ZIP protein, Mlx,
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13
Figure 3: An illustration of the major domains in ChREBP
ChREBP is a member of the bHLH/ZIP Leu-ZIP family of
transcription factors. It has been primarily linked to
the carbohydrate-induced activation of lipogenic gene
expression. Various regulatory phosphorylation sites
which have been well characterized in ChREBP cellular
localization and activation are denoted. Additionally, a
putative nuclear entry and nuclear localization site have been
identified near the N-terminus. (Adapted from
Kawaguchi et al., PNAS, 2001)
Glucose Sensing Module
N - - C
1 864
Ser196 Ser568 Ser626 Thr666
NLSNES Pro-Rich Pro bHLH ZIP ZIP-Like
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14
which interacts with the bHLH/ZIP domain of ChREBP (65). Mlx is
a member of the
Myc/Max/Mad family of transcription factors. This family serves
as a common interaction
partner within a transcription factor network (66). Evidence for
the interaction between Mlx
and ChREBP was provided through the use of a dominant-negative
form of Mlx (54).
According to the model proposed by the Towle group, two
ChREBP-Mlx heterodimers bind
to the two E boxes of the ChoRE in order to provide a
transcriptional complex necessary for
glucose regulation (54, 62, 63, 65). Interestingly, it appears
that the Mlx loop region, but not
the loop region of ChREBP, is the determinant for the regulation
of the glucose response, in
which ChREBP binds to the ChoRE found in lipogenic target genes
(63).
1.03.2 Regulation of ChREBP activity by post-translational
modifications:
Phosphorylation and De-phosphorylation
ChREBP has been shown to undergo post-translational
modifications which modulate its
subcellular localization and transcriptional activity. ChREBP
has also been demonstrated to
shuttle from the cytoplasm to the nucleus following stimulation
with high glucose (>25 mM)
(12). Later, it was shown that in primary culture, and in livers
from high-carbohydrate fed
mice, ChREBP is indeed concentrated in the nucleus when
activated (67). Based upon the
current model (Figure 4), ChREBP is located within the cytosol
under basal conditions.
Following a stimulatory event, such as high glucose
concentrations (>25 mM), xylulose-5-
phosphoate (Xu-5-P) selectively activates protein phosphatase 2a
(PP2A). PP2A then acts to
dephosphorylate ChREBP at the serine 196 (Ser-196) site,
allowing its nuclear translocation.
Upon entry into the nucleus, ChREBP is further dephosphorylated
by PP2A at the threonine
666 (Thr-666)
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15
Figure 4: Mechanisms underlying ChREBP
activation/inactivation
ChREBP is activated by an offshoot of the glycolytic pathway
Xu-5-P (derived from G-6-P). Xu-5-P up-
regulates the activity of PP2A which in turn dephoshorylates
ChREBP at the Ser196 site. This action allows
ChREBP to enter the nucleus where PP2A further dephosphorylates
the peptide at the Thr666 site, allowing
binding to the DNA with its partner Mlx. Fatty acids and
hormones have been shown to reverse this process
through PKA and AMPK as indicated on the right side of the
figure.
Transcriptional Activation of
Lipogenic targets
Cytoplasm
Nucleus
Xu-5-P Ser196Thr666
Ser196Thr666
Fatty Acids Hormones
cAMP
PP2A
PKA
ACS
AMP
AMPK
Glucose
PP2A
-
16
residue. This event allows the binding of ChREBP to the ChoRE
and thus results in the
activation of target gene expression.
Questions have been raised as to the accuracy of this model, as
mutants of ChREBP in
which one or several of the proposed phosphorylation sites have
been mutated (including
Ser-196 and Thr-666), retain the ability to activate target gene
expression in response to
glucose stimulation (68). It has been suggested that the
post-translational control of
ChREBP is complex, and may require mechanisms other than PKA
mediated
phosphorylation in order to regulate the activation of
downstream targets (69).
1.03.3 Role of ChREBP in Glucose Metabolism
ChREBP has been shown to activate the transcription of a large
set of targets involved in
glycolytic and lipogenic pathways, including L-PK ACC and FAS
(12, 70). Binding of ChREBP to
these target promoters was confirmed with chromatin
immunoprecipitation (ChIP) (55).
Knockdown of ChREBP, achieved with a siRNA approach, removed the
glucostimulatory
effect on the expression of L-PK, ACC, and FAS genes (70). As
illustrated in Figure 5, ChREBP
is required for both basal and carbohydrate-induced expression
of several liver-enzymes for
coordinated control of glucose metabolism and fatty acid
synthesis (18, 53, 55, 58, 63).
Studies using the ChREBP-/- mouse model, generated in 2004, have
revealed the role of
ChREBP under physiological and pathophysiological conditions
(71). As anticipated, ChREBP-
/- mice showed impaired glycolytic and lipogenic pathways in the
liver. These mice also
exhibited glucose and insulin intolerance (71), reduced
expression of mRNA of genes that
encode ME, ACC, FAS, and L-PK, along with decreased lipogenesis
(55, 71). Additionally,
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17
Figure 5: The role of ChREBP in metabolic regulation
The wide range of ChREBP target sites and the implications to
cellular metabolism has caused ChREBP to
become a target of great interest in research. ChREBP, bound to
the ChoRE of various target genes regulates
the expression of a variety of important enzymes in metabolic
processes. Well-characterized targets are
indicated here in yellow and include: SCD-1, FAS, ACC, ACL, ME,
and L-PK. (Adapted from Dentin et al.,
Biochimie, 2004).
Gly
coly
sis
Glucose
Glu-6-P
Fru-6-P
GAP
PEP
Pyruvate
Xu-5-P
Lipo
genesis
MUFA-CoA
SFA-CoA
Malonyl-CoA
Acetyl-CoA
Citrate
Acetyl-CoA
TCA Cycle
Malate Oxaloacetate
Transcriptional Activation of
Lipogenic targets
PP2A
Cytoplasm
Nucleus
-
18
these mice showed reduced fat pads, indicating that ChREBP may
play a function in that
tissue. There is corroborating evidence for this notion as
ChREBP expression was shown to
be induced during adipocyte differentiation in 3T3-L1 cells
(72). Lastly, ChREBP-/- mice
demonstrate a marked intolerance to fructose-rich diets
(71).
1.03.4 Transcriptional Regulation of ChREBP
The identification of ChREBP as a key hepatic glucose sensor
involved in channelling
carbohydrates into lipogenic pathways explained why in vitro
high ambient glucose alone
was sufficient in stimulating a panel of lipogenic genes;
independent of the well
characterized SREBP-1c (12, 17, 58, 59, 73-75). Recent
genome-wide association studies
have linked certain ChREBP polymorphisms with low ratios of high
density lipoprotein (HDL)
/ HTG levels, characteristic of subjects at risk for liver and
cardiovascular diseases (76-78). In
addition, the assessment of ChREBP-/- mice showed that despite
normal SREBP-1c
activation, the expression of mRNA that encode enzymes required
for lipogenesis, were
reduced (54, 55, 71).
Although the stimulatory effect of glucose on the function of
ChREBP in activating
lipogenic gene expression has been extensively investigated (23,
75), we know very little
about mechanisms underlying the expression of this master
controller of lipogenesis.
Several recent studies suggested that high ambient glucose,
insulin, PPARγ agonist, Liver X
receptor (LXR) and polyunsaturated fatty acids (PUFAs) may also
affect ChREBP mRNA
expression levels (17, 23, 70, 75, 79, 80). Whether these
components affect ChREBP
expression at the gene transcriptional level, however, has not
been investigated.
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19
Furthermore, in the hyperinsulinemic ob/ob mouse model, ChREBP
expression level is
substantially higher, indicating a potential role of insulin in
stimulating ChREBP production
(61, 81).
A recent study examined the cis-regulatory elements of the rat
ChREBP gene promoter
(82). Although this study revealed a few nuclear protein binding
sites, including those for
the transcription factors Sp1 and NF-Y, on the rat ChREBP
promoter, how transcription
factors mediated physiological and pathological signals in
regulating ChREBP transcription
was not examined. Furthermore, there is almost no information
regarding the mechanisms
underlying the expression of the human ChREBP (hChREBP) gene
promoter. More recently,
Hashimoto et al demonstrated that the thyroid hormone is able to
activate ChREBP
promoter activity (83). This activity, however, appeared to be
dependent on coordination
with LXRs to regulate ChREBP transcription. As ChREBP has been
identified as a labile
protein with a short half-life of 30 minutes (84), understanding
the players involved in its
transcription is of great importance to better understand the
role of ChREBP in human
diseases. In this study, we show the existence of an
evolutionarily conserved POU protein
binding site within the proximal region of the ChREBP promoter
that interacts with the
ubiquitously expressed POU protein Oct-1.
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20
1.04 The POU Homeodomain Protein Oct-1
1.04.1 POU Domain Superfamily
Oct-1 is a member of the POU homeodomain superfamily of highly
conserved eukaryotic
transcription factors involved in the regulation of embryonic
development and other bio-
physiological functions. The POU domain was first identified in
1988 and takes its name
from the first four POU transcription factors in which it was
found: Pituitary-Specific
Transcription Factor-1 (Pit-1); The Octamer-Binding
Transcription Factors, Oct-1 and Oct-2;
and the Neural Unc-86 Transcription Factor (Unc-86 was derived
from Caenorhabditis
Elegans) (85-88). The POU domain was distinguished from other
homeodomains by a region
found N-terminal to it; which, when first identified had no
known homologues. Since the
two sub-domains (POUS and POUHD) were separated by a variable
linker sequence (87), it
was suggested that the POU domain was bipartite in nature.
Figure 6A demonstrates the
two subdomains of the POU domain for the POU family member
Oct-1. POU proteins are
characterized based upon the presence of a bipartite DNA binding
domain referred to as the
POU domain. This domain consists of both a homeodomain and a
helix-turn-helix structure,
joined by a flexible polypeptide “leash” or linker (Figure 6B).
The entire protein is encoded
in a single gene and is synthesized as a continuous polypeptide
chain. The covalent joining
of two structures in this way results in a large increase in the
affinity of the protein for its
specific DNA sequence compared with the DNA affinity of either
separate structure.
This structurally bipartite POU domain has an intrinsic
conformational flexibility, which
appears to confer a functional diversity to this particular
class of transcription factors (89).
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21
Figure 6: The overall domain structure of Oct-1
Oct-1 is a member of the POU homeodomain superfamily of
transcription factors. Oct-1 is the only
ubiquitously expressed member of this family, and has been
implicated as a potent positive or negative
regulator of a large variety of target genes. (A) This family is
characterized by the POU domain closely linked to
a homedomain which allows a great specificity to target
sequences by “wrapping” around the DNA sequence.
(B) The structure shown (called a POU-domain) consists of both a
homeodomain and a helix-turn-helix
structure joined by a flexible polypeptide “leash,” indicated by
the broken lines (Adapted from Alberts et al,
the Molecular Biology of the Cell, 4th Edition, 2002).
0 755
POU HD
LinkerActivation
Domain
B
A
Homeodomain
POU Domain
Linker
-
22
POU domains are an example of a classic modular molecular
construction, utilizing
ancient folds used in transcriptional control within prokaryotes
and early eukaryotes and
adapting them to regulatory processes in highly specialized
tissues within complex
organisms. Interestingly, this modular structure imparts a
functional versatility that allows
the POU domain to participate in transcriptional regulation of a
variety of target genes.
POU domain proteins have been grouped based upon the properties
of the linker that
separates the two DNA-binding domains (90-92). The seven major
POU domain families,
defined by their linker sequences are listed in Table 1.
1.04.2 The Octamer Binding Proteins
The ubiquitously expressed Oct-1 and its closely related,
tissue-specific form Oct-2 were
identified as proteins which target the octamer regulatory
element ATGCWAAT (W=A/T)
(93, 94). Oct-2 is mainly expressed in B lymphocytes and plays a
much more specialized role
than Oct-1; being responsible for regulation of immunoglobulin
gene expression (95, 96).
This effect, however, is to some extent redundant, as Oct-1 can
act as a functional
substitute to Oct-2 to regulate B cell immunoglobulin gene
expression (97-99).
Oct-1 was identified early-on to interact with a variety of
other transcriptional
regulators in order to activate the transcription of a variety
of genes, including snRNA (100),
and histone H2B (101). It is also used by the herpes simplex
virus to activate the expression
of the viral immediate early (HSV IE) genes (102). Oct-1
activates transcription of target
genes by various means. For example, the snRNA genes,
transcribed by two RNA
polymerases, pol II and pol III, are activated via Oct-1
behaving as an accessory factor to
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23
Type Characteristics Examples
I Neuronally expressed Pit-proteins
II Important in development and cellular stress response Oct-1,
and Oct-2
III Involved in neural development Brn 1, 2, 4 and Tst-1
IV Involved in development Brn 3.x class, Unc-86
V Involved in early embryogenesis Oct-4
VI Expressed in the central nervous system Brn 5, MPOU
VII Critical for early development stages POU-2
Table 1: An overview of the various identified forms of POU
homeodomain proteins
Table adapted from references (92, 103).
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24
locate the activation complex which is identical for both
polymerases (104). In other
cases, Oct-1 has been shown to act in synergy with recruited
factors in order to up-regulate
gene transcription.
The Oct-1 POU domain complexed with the octamer site from the
H2B promoter
revealed the detailed interactions underlying the DNA
recognition element (105). The two
subdomains of the POU domain make independent interactions with
two half-sites on the
operator and are bound on opposite faces of the DNA. The ATGC
sub-site of the octamer
element is contacted by the POUS domain with an extensive array
of hydrogen bonds while
the WAAT site tends to bind by the POUHD domain. The role of the
linker region in POU
proteins has been postulated to be specific for promoter
recognition and co-operative
effects (106, 107). Additionally, POU proteins have increased
sequence-specific DNA
recognition through their ability to homo and heterodimerize
(108, 109). The net result of
this highly stringent design is a transcription factor with the
potential to effectively regulate
a large series of targets with high efficiency.
1.04.3 The Function of Oct-1
Extensive examinations have shown that Oct-1 may function as a
transcriptional
activator or a repressor (82, 110-129) for a large profile of
genes. While Oct-1-/- mice are
embryonically lethal (130), studies on fibroblasts isolated from
Oct-1-/- mice, demonstrated
that this POU protein plays an important role in regulating the
expression of genes that
mediate the cellular stress responses (69, 119, 131, 132).
Oct-1-/- fibroblasts were
hypersensitive to gamma radiation, doxorubicin, and hydrogen
peroxide stresses (132).
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25
Interestingly, these cells harboured elevated reactive oxygen
species and demonstrated
altered expression of genes associated with the cellular stress
response (120, 132-134).
These observations indicate that Oct-1 is an important stress
sensor and a key part of the
cellular response pathway to stress.
Oct-1 and Oct-2 DNA binding site specificity has been shown to
be regulated in vitro by
different kinases (135). Phosphorylation of Oct-1 by PKA
activation has been shown to
inhibit its DNA binding activity in nuclear extracts of Daudi
cells. It is thought that
differential binding of Oct-1 to the octamer sequences may be
possible through regulation
of its phosphorylation (135). In addition, PP2A, a positive
regulator in ChREBP activation,
has been implicated as a negative regulator of Oct-1 DNA binding
to the H2B promoter
(135). Additionally, a recent study has identified a series of
serine and threonine sites on the
Oct-1 peptide which are phosphorylated following oxidative
stress (136).
1.04.4 Oct-1 and Metabolic Stress
The POU homeodomain superfamily of transcription factors are
involved in an ever-
expanding variety of regulatory mechanisms. More recently, Oct-1
was shown to mediate
the effect of oxidized LDL (oxLDL) in repressing the expression
of vascular cytochrome P450
(CYC) monooxygenases (119). In human coronary arterial
endothelial cells, knockdown of
Oct-1 expression prevented oxLDL-mediated silencing of CYP
expression (119).
Furthermore, inhibition of oxLDL receptor (LOX-1) attenuated
oxLDL-mediated endothelial
DNA damage, Oct-1 DNA binding, and reversed impaired production
of endothelial-derived
hyperpolarization factor (119). Therefore, Oct-1 activation in
response to oxidative stress is
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26
among the pathological responses in metabolic dysfunction of
coronary arterial
endothelium (119). With the trends in public health towards
obesity and the increasing
prevalence of many aspects of the metabolic syndrome, the
stresses placed upon the
body’s cells and regulatory pathways are ever-increasing.
It is therefore likely that transcription factors such as Oct-1
play an important role for a
large number of these pathophysiologic processes. Dysregulation
or altered function of Oct-
1 which has been clearly demonstrated to greatly impact its
downstream targets. Our
observations presented here indicate that Oct-1 plays an
important role in the molecular
control of hepatic lipogenesis through its interaction with the
promoter region of ChREBP.
As a repressor of lipogenic induction, and a sensor of
physiological changes to stress, Oct-1
may prove to be an important element in the control of a variety
of human diseases.
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27
Chapter 2. RATIONALE AND HYPOTHESIS
Studies focussing on ChREBP have demonstrated that this
transcription factor plays an
important role in regulating hepatic lipogenesis. While the
post-translational regulation and
function of ChREBP has been extensively investigated, very
little is known about the
transcriptional regulation of this important gene. An
understanding of the mechanisms
involved in the mechanistic control of lipid production would
greatly aid in the development
of treatments for, and the understanding of, a variety of
pathological conditions, including:
obesity, T2DM, NAFLD and the metabolic syndrome. Thus, an
understanding of the basic
molecular mechanisms which underlie lipid biosynthetic process
activation is most
desirable, indeed it is imperative.
Previous studies of the POU homeodomain protein Oct-1 in Dr.
Jin’s laboratory indicated
that it was a transcriptional repressor of another homeodomain
transcription factor, the
cdx-2 gene. We thus hypothesized that Oct-1 would act to
negatively regulate ChREBP gene
expression. While ChREBP has been demonstrated to be
post-translationally regulated by
various factors, few studies have investigated the
transcriptional regulation of its
expression. Interestingly, studies in hyperinsulinemic mice
indicated that ChREBP mRNA and
protein levels were significantly increased (40, 41). Glucose,
however, has not been shown
to significantly upregulate ChREBP mRNA expression. Thus we
sought to determine whether
insulin affected ChREBP gene expression. Additionally, very
little is known about the effect
of insulin on ChREBP as studies have focussed on the
glucose-response of this important
transcription factor. We hypothesized that insulin stimulation
would increase ChREBP
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28
expression, and that this increase in expression may be, at
least in part, due to an
attenuation of the repressive effect of Oct-1 on gene
expression.
2.01 Whether Oct-1 Functions as a Repressor of ChREBP
Expression
The first aim of our study was to assess the ChREBP promoter and
its interaction with
the POU homeodomain protein Oct-1. This aim was focussed to
identify the first potential
transcription factor acting on the expression of the ChREBP
promoter. Until recently,
lipogenesis was understood to be activated primarily through the
SREBP family, and in
particular, SREBP-1c (137-139). With the discovery and
characterization of ChREBP in 2001
(12, 53), the field of lipogenic regulation opened to include a
carbohydrate-driven element.
When we started this line of investigation, there had been only
limited studies which
attempted to examine the ChREBP promoter region and its
transcriptional regulation (82).
Our project began with the identification of a highly-conserved
POU binding site within
the human ChREBP proximal promoter, specific for the POU
homeodomain proteins. Oct-1
has been shown to act as a potent transcriptional regulator of
various genes, and has been
linked to important cellular metabolic pathways; in particular,
the cell’s stress response
system. With this knowledge, we tested our first hypothesis that
Oct-1 acts as a
transcriptional repressor of ChREBP gene expression. Indeed, we
found through a variety of
experimental formats that Oct-1 is a negative regulator of
ChREBP promoter and Oct-1
over-expression repressed ChREBP mRNA and protein
expression.
-
29
2.02 Whether Insulin Treatment Stimulates ChREBP Expression
The second aim of our study was to examine the effect of insulin
on ChREBP gene
expression. There is evidence that the expression of ChREBP, its
function primarily induced
by carbohydrates, is also elevated in states of
hyperinsulinemia. Studies in hyperinsulinemic
mice demonstrated that ChREBP mRNA and protein levels are
increased compared to
control animals (40, 41). We therefore proposed that insulin
acts to stimulate ChREBP gene
expression. Within this aim, we chose not to assess chronic
insulin stimulation, but rather
the effect of acute insulin treatment on ChREBP gene expression.
This allowed us to make
effective use of the protocols and cell lines utilized in the
first aim, and also allowed a
standpoint from which we would approach our third aim (see
below). Observations from
our second aim demonstrate that insulin acts as a stimulatory
agent of the expression of
ChREBP promoter, mRNA and protein levels. When we coupled our
insulin stimulation
experiments with the Oct-1 knockdown cell line, and Oct-1
over-expressing cell lines,
generated for Aim 1, we further characterized the insulin
stimulation as possibly being
influenced by Oct-1 content.
2.03 Whether Insulin Stimulates ChREBP Expression via
Attenuating the Effect of
Oct-1 on the ChREBP Promoter
The third aim of our study was an extension of the first
elements in order to assess the
potential for interaction between Oct-1 and insulin with regard
to ChREBP gene expression.
Since aim 1 sought to identify a potential negative regulator,
and aim 2 sought to identify a
potential positive regulator, it was likely that there would be
some interface between the
two elements in order to achieve their effect on the ChREBP
promoter. The hypothesis for
-
30
our third aim was that insulin acted to stimulate ChREBP
expression via modulating the
repressive effect of Oct-1 on the ChREBP promoter. In fact,
using a variety of techniques, we
demonstrated that insulin appeared to exert its stimulatory
effect, at least in part, by
decreasing the Oct-1 content, and thereby releasing the
repression of Oct-1 on the ChREBP
promoter.
All three aims and hypotheses of this study are summarized in
Figure 7.
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31
Figure 7: An illustration of the hypotheses of this study
A recapitulation of the mechanisms involved in activation of
hepatic lipogenesis. A) Oct-1 binding to the
POU site located in the ChREBP promoter (human position -96 to
-103 bp) results in transcriptional repression
of ChREBP. B) Insulin acts to stimulate ChREBP expression via
attenuating the repression of the ChREBP
promoter by Oct-1. Insulin may cause a phosphorylation event
excluding Oct-1 from the POU site present in
the ChREBP promoter. The conserved POU binding site ATGCTAAT is
denoted on the double helix in orange
P P
A
B
Oct-1
ChREBP Expression
Oct-1
ChREBP Expression
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32
Chapter 3. MATERIALS AND METHODS
3.01 Chemicals
The antibiotics G418 (invitrogen Life Technologies, Burlington,
Ontario, Canada) and
Puromycin (Sigma-Aldrich Canada Ltd., Oakville, Ontario, Canada)
were used to select cell
lines to maximal transfection or infection efficiency for the
shRNA mediate gene knockdown
approach and Oct-1 over-expression approach. The MEK/ERK
inhibitor PD98059, the AKT
1/2 inhibitor, and the PI3k inhibitor LY294002 were purchased
from Calbiochem (EMD
Biosciences, Inc., San Diego, California, USA). Cells were
starved in serum-free medium
overnight, and then pre-treated with either the MEK or AKT
inhibitor for one hr prior to
experimentation. DMSO treatment was used as a control in each
experiment. All other
chemicals were purchased from Sigma-Aldrich.
3.02 Plasmid Construction
A 1.4 kb human ChREBP (hChREBP) gene 5’ flanking sequence was
isolated from the
human hepatocellular carcinoma cell line HepG2 by Dr. Ling Liu
in Dr. Jin’s laboratory. The
sequence information has been deposited into the Genbank (Access
number: FG480402).
Four human ChREBP (hChREBP)-luciferase (LUC) fusion gene
constructs were generated
using a nest deletion strategy, inserting various lengths of the
5’ flanking region of hChREBP
into a pPGL2 basic (Promega/ Fischer Scientific, Nepean,
Ontario, Canada) plasmid
construct. The constructs were designated as -1.4 kb
hChREBP-LUC, -328 bp hChREBP-LUC, -
123 bp hChREBP-LUC (wt), and -123 bp hChREBP-LUC (mut). As
illustrated in Figure 10A, all
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33
fusion gene constructs contain the wild-type Oct-binding site
(ATGCTAAT) with the
exception of -123 bp hChREBP-LUC (mut) in which the Oct-binding
site was mutated
(ATACGGAT).
The plasmid constructs pcDNA3.1-Oct-1 and pcDNA3.1-Cdx-2 were
generated by
inserting a copy of the Oct-1 or Cdx-2 coding sequence into a
pcDNA3.1/Myc/HisA
expression vector (Invitrogen Life Technology).
3.03 Cell Culture
The HepG2 cell line was cultured in α-MEM (Invitrogen Life
Technologies), enriched with
5% fetal bovine serum (FBS), and cultured without antibiotics
for all of the experiments.
Cos-7 fibroblasts were cultured in DMEM-Low (Invitrogen Life
Technologies) containing 5%
FBS. All tissue culture plates were incubated at a temperature
of 37°C enriched with 5%
CO2. Primary hamster hepatocytes were isolated in collaboration
with the Khosrow Adeli
Lab (Hospital for Sick Children, Toronto, ON). Methods for
isolating and culturing primary
hepatocytes from Syrian Golden Hamsters have been reported
previously (140, 141).
Briefly, livers from anaesthetized (isoflurane) male hamsters
weighing 100-120 g (Charles
River, Montreal, QC) were perfused and digested using
specialized media (Life Technologies,
Inc.) according to the manufacturer’s instructions. Hepatocytes
released from digested liver
tissue were washed twice in hepatocyte wash medium, resuspended
in attachment media
(Williams E containing 5% FBS, 1 ug/ml insulin, and antibiotics)
and seeded in Primaria cell
culture plates (BD Biosciences) at a density of 1.1 x 106
cells/35 mm dish. Following a 4 hr
attachment period the cells were cultured in serum free α-MEM
for 4 hr prior to insulin
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34
treatment. All animal protocols were approved by the animal
ethics committee at the
Hospital for Sick Children, Toronto, Ontario.
3.04 DNA Transfection and Luciferase Reporters
Transfection of DNA plasmids was achieved utilizing
Lipofectamine 2000. Cells were
cultured in serum-free medium, one hr prior to transfection. DNA
and Lipofectamine 2000
were used in a 2:1 ratio (i.e. 2µg DNA : 1µl Lipofectamine
2000). Transfection was allowed
for 16-18 hrs followed by treatment and cell harvesting.
Luciferase activity was analyzed
using an EG&G Berthold Lumat LB 9507 Luminometer, and
relative luciferase activity was
normalized to the protein concentration in each extract. SK-LUC
and CMV-LUC were used as
positive and negative controls, respectively, in each
experiment. Additionally, a pool of
HepG2 cell clones that over-express Oct-1 were generated by
stably expressing pcDNA3.1-
Oct-1 with antibiotic (G418) selection.
3.05 Lentiviral shRNA Knockdown
Lentiviral knockdown of Oct-1 was achieved using the pGIPZ
Lentiviral shRNAmir system
(ThermoFischer Scientific, Huntsville, Alabama, USA), in which,
GFP expression serves as the
indication of infection efficiency. A helper packaging and
enveloping plasmid were co-
transfected with each of the individual shRNA constructs in the
HEK293T cell line. Following
72 hrs of lentiviral growth and secretion, the medium containing
the virus was harvested
and the titre was determined. HepG2 cells were then infected
with each of the three shRNA
constructs for Oct-1 or the shRNA construct for the scrambled
control. Infection was
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35
allowed for 72 hrs and then cells were selected to 100%
infection efficiency with the use of
the antibiotic agent puromycin.
3.06 RNA Extraction and Real Time RT-PCR
RNA was extracted using Trizol reagent (Invitrogen Life
Technology) as per
manufacturer’s instructions. cDNAs were generated using the
Superscript First Strand
Synthesis System for RT-PCR (Invitrogen Life Technologies).
Real-time RT-PCR was
conducted with Rotor-Gene 3000 (Corbett Research, Dorval,
Quebec, Canada), utilizing the
SYBR Green PCR Master Mix (Applied Biosystems, Foster City,
California, USA). DNA
sequences of the primers for real time RT-PCR in detecting
hChREBP mRNA are: forward: 5’-
GGTCACTTCATGGTGTCGTC -3’, and reverse: 5’- CACATCTGTAGGCCAGGCT
-3’. DNA
sequences for the primers for real time RT-PCR in detecting L-PK
are: forward: 5’-
GAGTCGGAGGTGGAAATTGT -3’, and reverse: 5’- CCGCACCACTAAGGAGATGA
-3’.
Expression levels of ChREBP mRNA are presented as relative copy
numbers (with the
untreated samples defined as 1), normalized to the expression of
β-actin mRNA.
3.07 Chromatin Immunoprecipitation
HepG2 cells (90% confluent) were treated with or without 10 nM
insulin for 4 hrs.
Following the treatment, the cells were exposed to 1%
formaldehyde for 10 min at room
temperature. Glycine was added to neutralize the solution to a
final concentration of 0.125
M for 5 min. The samples were then washed twice with cold PBS
and then harvested in PBS.
Cells were collected by centrifugation, and resuspended in lysis
buffer (5 mM HEPES (pH
8.0), 80 mM KCL, 1% NP40, 100 mM PMSF, and protease inhibitors).
Following 10 min of cell
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36
lysis, nuclei were pelleted and resuspended in the nuclear lysis
buffer (50 mM Tris-Cl (pH
8.0), 10 mM EDTA, 1% SDS, proteinase inhibitors). Chromatin in
the harvested samples was
sonicated to an average length of 600 bp and separated by
centrifugation. The sheared
chromatin was cleaned with blocked salmon sperm DNA/protein A
agarose for 30 min at
4°C. The cleaned chromatin was separated from other products by
centrifugation and
stored in a clean eppendorf. A portion of the cleaned chromatin
was diluted 10 fold with
the IP dilution buffer (0.01% SDS, 1.1% Triton 100, 1.2 mM EDTA,
16.7 mM Tris-Cl (pH 8.0),
167 mM NaCl, protease inhibitors), and was incubated overnight
(with rotation at 4°C) with
the Oct-1 antibody. Blocked salmon sperm DNA/protein A agarose
was added to the IP
pellet and incubated for 3 hrs at 4°C with rotation. The IP
product was collected by
centrifugation and washed sequentially in: low salt wash buffer
(0.1% SDS, 1% Triton X-100,
2.0 mM EDTA, 20 mM Tris-Cl (pH 8.0), 150 mM NaCl, protease
inhibitors), high salt was
buffer (0.2% SDS, 1% Triton X-100, 2.1 mM EDTA, 20 mM Tris-Cl
(pH 8.0), 500 mM NaCl,
protease inhibitors), and a LiCl immune complex washing buffer
(0.25 mM LiCl, 1% NP40, 1
mM EDTA, 10 mM Tris-Cl (pH 8.0), 1% deoxycholate, protease
inhibitors). A portion of this
sample was saved for Western Blot analysis. The remaining sample
was reverse cross linked
with TE/1%SDS and heated at 65°C overnight. Following
incubation, proteinase K (10
mg/ml) was added and the sample was incubated at 37°C for 2 hrs.
The DNA product was
then diluted with buffer PB (Qiagen PCR Purification Kit), and
run through a suspension
column. The column was washed with buffer PE (Qiagen PCR
Purification Kit), and finally
eluted in ddH2O. DNA sequences of the primers for the
amplification of the OCT binding site
containing hChREBP promoter region are: forward: 5’-
CAGGACTCCAAGGAAAGACG -3’, and
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37
reverse: 5’- GTCTGTGTCCGAGTCCGAGT -3’. DNA sequences of the
primers for detecting the
intron product (contains no OCT binding site, serving as the
negative control) are: forward:
5’- AGGGCATCTAAGGTCCTGGT -3’, and reverse: 5’-
CCCCAGCTATCTCTGACTGG -3’.
Expression levels are presented as relative copy numbers (with
the untreated samples
defined as 1) and normalized to the input values.
3.08 Nuclear Protein Extraction
The method for nuclear extraction has been described previously
(142). Briefly, nuclear
extraction buffers were prepared containing: Buffer A: 10mM
HEPES (pH 7.9), 10mM KCl,
0.1 mM EDTA, 0.1 mM EGTA, 1mM DTT, and 0.5 mM PMF, Buffer C:
20mM HEPES (pH7.9),
0.4 mM NaCl, 1mM EDTA, 1mM EGTA, 1mM DTT, and 1mM DTT. Cells
were pelleted and
then lysed with buffer A. 25 µl NP-40 was added and cells were
vigorously vortexed. Nuclei
were pelleted, and the cytosolic fragment transferred to a fresh
eppendorf tube for storage.
The nuclear pellet was lysed with buffer C, and nuclear
fragments pelleted. The supernatant
containing the nuclear content was transferred to a fresh
eppendorf tube and stored prior
to blotting.
3.09 Immunoblotting
The cell lysis buffer contained 10% NP-40, Sodium Deoxycholate,
and 10X SDS.
Immediately prior to use, a protease inhibitor tablet, (Complete
Mini, EDTA-free) (Roche
Pharmaceuticals, Indianapolis, IN, USA) was added. Following
lysis, the cellular fragments
were pelleted and the supernatant was transferred to a new
eppendorf tube which
contained an appropriate volume of 10X SDS loading dye. For
immunoblotting, equal
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38
amounts of total cellular protein were separated by SDS/PAGE,
transferred to immobilon,
and immunoblotted with antibodies following the protocols
provided by manufacturers.
Immunocomplexes were visualized by an enhanced chemiluminescence
detection kit (Super
Signal West Pico Chemiluminescent Substrate) (Thermo Scientific,
Rockford, IL, USA) by
using horseradish peroxidase-conjugated secondary antibodies
(Santa Cruz Biotech,
California, USA). Antibodies against Oct-1, GSK-3,
phospho-GSK-3, ERK, phospho-ERK, as
well as β-actin were purchased from Santa Cruz Biotechnology
Inc. (Santa Cruz Biotech). The
antibody for ChREBP was purchased from Novus Biologicals (Novus
Biologicals, Littleton,
Colorado, USA).
3.10 Electrophoretic Mobility Gel Shift Assay (EMSA)
The POU-binding site containing probe (ChREBP-POU probe) was
made by annealing
two complementary oligo nucleotides: ChREBP-POU-forward: 5’-
GATCTGCGTAAGGATTATGCTAATACAAGCCCCGCG -3’, and ChREBP-POU-
reverse: 5’-
GATCCGCGGGGCTTGTATTAGCATAATCCTTACGCA -3’. The position of this
probe in the
hChREBP promoter is indicated in Figure 9. The mutant
POU-binding site containing probe
(ChREBP-POU mut) was made by annealing the following two
complementary oligo
nucleotides: ChREBP-POU mut forward: 5’-
GATCTGCGTAAGGATTATACGGATACAAGCCCCGCG -3’, and ChREBP-POU
reverse: 5’-
GATCCGCGGGGCTTGTATCCGTATAATCCTTACGCA- 3’. The EMSA was performed
by
incubating approximately 50x103 (cpm) end-labeled DNA probe
(0.25ng/reaction) with
nuclear protein in binding buffer (10 mM Tris-HCI (pH 8.0),40 mM
KCl, 6% glycerol, 1 mM
-
39
dithiothreitol, and 0.05% NP-40) for 30 min at 30°C. Each
incubation also contained 2-8 µg
poly-dI/dC and the PCR generated fragment was used as a
nonspecific competitor DNA. The
reaction was then loaded onto a 5% nondenaturing polyacrylamide
gel. After the
electrophoresis, the gel was dried prior to exposure to an X-Ray
film for 16-24 hrs.
3.11 Statistical Analysis
Quantitative results are expressed as a mean ± the standard
deviation (SD). Statistical
analyses were performed using the student’s t-test or ANOVA.
Null hypotheses were
rejected at p > 0.05. All experiments were performed
independently as a minimum of
triplicates.
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40
Chapter 4. RESULTS
4.01 Oct-1 as a negative transcriptional repressor of ChREBP
expression
4.01.1 Oct-1 interacts with the POU protein binding site located
within the
proximal promoter region of ChREBP
Following the isolation and examination of the 1.4 kb human
ChREBP gene 5’ flanking
sequence, we identified a typical POU protein binding site at
the position between -103 bp
and -96 bp. This octamer motif (ATGCTAAT) and the flanking
sequences were found to be
conserved between human and rodent (mouse and rat) species
(Figure 8). Importantly, we
have also examined the human SREBP-1c gene promoter and could
not locate a POU
protein binding site. Except for the ubiquitously expressed
Oct-1, no other POU
homeodomain proteins are abundantly expressed in the
hepatocytes.
To examine whether Oct-1 interacts with this binding site, we
conducted EMSA using
nuclear proteins isolated from the human HepG2 cells against the
probe designated as
ChREBP-POU. As shown in lane 2 of Figure 9A, HepG2 nuclear
proteins formed two
complexes with the ChREBP-POU probe: a major complex C1 and a
minor complex C2. To
investigate whether one of them represents the interaction
between Oct-1 and the ChREBP-
POU probe, Oct-1 antibody and a control GATA-1 antibody were
utilized in the EMSA. The
pre-incubation of nuclear proteins with the Oct-1 antibody (lane
3) but not with the control
GATA-1 antibody (lane 4) resulted in a retarded migration of C1,
suggesting that the major
complex C1 contains Oct-1.
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41
Figure 8: The POU-binding site is highly conserved within ChREBP
promoters
An illustration of the rat, mouse, and human ChREBP proximal
promoter regions. POU, the evolutionarily
conserved POU protein binding site. The sequence ATGCTAAT was
found to be specific for the POU protein
Oct-1. TATA, TATA box. TSS, transcription start site. DNA
sequence information was obtained from Genbank
(NM_032951, human; NM_133552, rat; and NM_021455, mouse).
Rat ----GAAAAGTAGGGACTA----AAAATTGCTCACA------GCAGACAGAAAGGTTAAG
-152
Mouse
----GAAAAGTAGAGACTA----AAAAGTGCTCTCA------ACAGAAAGAAAGGCGAAG
-167
Human
TGGAGCAAAGTAGGGGCGGCTCTATCAGGACTCCAAGGAAAGACGGGAAGGGAGATGGGG
-154
* ******* * * * * *** * * * ** ** *
Y-box POU
Rat C---------------CAGGACCAGTGGTTTCACACCAGGCTCATTGGTTATGCTAATTT
-107
Mouse
CA--CCGCCAAAGCTCCAGGATCAGTGGTTTCACACCACGCTCACTGGTTATGCTAATTT
-109
Human
CCAACCCGCCAGAGCTCCAGAGCACCGGGTTCTCACCTGGCGTAAGGATTATGCTAATAC
-94
* * ** ** ** *** **** ** * * **********
SRE SP-1
Rat AAACATCACCTGCCAACCAAGA-CCACGCCCACCACAAGGACAAAT-AGTGGGGCAGAGG
-48
Mouse
AAACATCACCTGCCAACTAAGGGCCACGCCCACCACAAGGACCTATCAGGAGAACAGGGG
-49
Human
AAGCCCCGCC--CCGTCCCAGG-CCCCGCCCACCCGATCGGCCTAT----GGTGTGGGGG
-41
** * * ** ** * ** ** ******** * * * ** * * **
TATA box TSS
Rat CGTGGCCATGGTAATTAGGCTCAGCTGGCAAGGATTGGCTCTCAAACTGCTAT-------
+5
Mouse
CGTGGCCATGGTAATTAGGCGCAGCTGGCTACGTTAAGCGCTCTCCAGAGCGC------- +4
Human
CGGGGCTATGTTAATGAGGCCCGGCTGGCCAAGAGCTGTTCCCCGCGCTGCGCGGAGCAG
+20
** *** *** **** **** * ****** * * * * *
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42
Figure 9: Oct-1 binds to the POU-binding site located within the
ChREBP promoter
EMSA shows the interaction between HepG2 nuclear proteins and
the POU binding site probe. (A) EMSA
identifying C1 as the Oct-bound probe through the use of Oct-1
Ab. (B) Extended separation by un-denatured
PAGE further confirms the existence of the supershifting with
the Oct-1 Ab. C1, complex 1; C2, complex 2; FP,
free probe, and NE, nuclear extract.
Supershift
C1
C2
1 2 3 4 5 6 7 8
NE - + + + + + + +
Oct-1 Ab (µl) - - 1 2 - - - -
Competitorwt
- - - - - -100
x200
x
Competitormut
- - - -100
x200
x- -
B
NE - + + +
Oct-1Ab
- - + -
GATA-1 Ab
- - - +
C1
Supershift
A
C2
FP
1 2 3 4
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43
The retardation of the C1 complex in the presence of Oct-1
antibody in the un-
denatured PAGE was minimal (Figure 9A). To further verify that
this does represent a
“supershifting” event, we extended the separation time of the
PAGE, and observed with
two different dosages of Oct-1 antibody a clear retardation in
the migration of the C1
complex (Figure 9B, comparing lanes 3 and 4 with lane 2).
Furthermore, the addition of an
excess amount of unlabelled ChREBP-POU probe (lanes 7 and 8),
but not the mutant one
(lanes 5 and 6), completely blocked the formation of the C1
complex, indicating that the
ATGCTAAT motif is essential for the formation of C1. We
therefore conclude that Oct-1
binds to the evolutionarily conserved POU protein binding site
in the human ChREBP gene
promoter.
4.01.2 Oct-1 functions as a transcriptional repressor of
ChREBP
To investigate whether the binding of Oct-1 to the hChREBP
promoter affects ChREBP
gene transcription, LUC reporter gene analyses