Mu 1: BO CO TM TT
M TI:
TN TI : NGHIN CU QUY TRNH PHN LP CC CHT T CY GI (EXCOECARIA
AGALLOCHA L., EUPHOBIACEAE)
Sinh vin:H v tn Lp Kha
Gio vin hng dn:Hc hm. hc v. H v tn
Khoa/Vin ................................ in thoi nhm SV :
Phn Tm tt ni dung ti:Bi cnh v mc tiu : Cy Gi c tn khoa hc l
Excoecaria agallocha L., h Euphorbiaceae l mt loi cy mc cc vng t bi
ven bin, ven sng a t ct pha, c mn, nc l hoc nc mn, cy rt d tm v c
kh nng pht trin tt. T Cy Gi (Excoecaria agallocha L.) ngi ta chit c
mt s cht c hot tnh khng virus HIV, nhiu hp cht c hot tnh sinh hc
cao, c tc dng c ch mnh i vi mt s loi virus, c kh nng khng vim, chng
oxy ha v c ch mt s tc nhn gy ung th.[1] V vy ti Nghin cu chit tch v
phn lp mt s cht t cy gi (Excoecaria agallocha L.) h Euphorbiaceae
Vit Nam s gp phn tm hiu tim nng khai thc v s dng loi cy hu ch
ny.Phng php: Bn mng v sc k bn mng, ct v sc k ct.Kt qu : T cn chit
n-hexan ca dch chit tng methanol ca loi Excoecaria agallocha L. thu
c hp cht EA1 (14-taraxerene-3-one) , EA2 (14-taraxerene-3-ol) , EA3
(14-taraxerene-3-ol).Kt lun :
TI LIU THAM KHO1.
............................................................................................................................................1.
............................................................................................................................................1.
MU 2: BN BO CO Y CNG TRNH D THI SVNCKH CP TRNGB GIO DC V O TOTRNG I
HC BCH KHOA H NI------o0o------
CNG TRNH THAM DGII THNG SINH VIN NGHIN CU KHOA HC CP TRNGNM
2015
Tn cng trnh: M s:H v tn sinh vin:Nam, N Lp, kha: Khoa: Tel:Gio
vin hng dn:
I. t vn 0. Thc vt chi ExcoecariaCy Gi (Excoecaria agallocha L.)
c vng phn b tng i rng, c th gp cc khu vc ven bin t min Nam n , Sri
Lanka, Myanmar n Thi Lan, Vit Nam V pha Bc cn gp Trung Quc, Nht Bn.
Pha Nam, c th gp Bc Austalia v khu vc Thi Bnh Dng . L cy c th sc
ung cha ng kinh; gi ti p tr cc vt lot. Dch l nu vi du dng xoa p tr
thp khp, phong v bi lit. R cy t c hn cc phn ca cy trn mt t. Gi ra
ln vi Gng, dng lm thuc chm tr sng chn tay. Ht phi nng c th ch du
dng tr gh.[2]Theo thng k, h Thu du (Euphorbiaceae) l mt h ln cathc
vt c hoavi 240 chi v khong 6.000 loi. Phn ln lcy thn tho, nhng khu
vc nhit i cng tn ti cc loicy bihoccy thn g. Mt s loi cy cha nhiu nc
v tng t nh cc loixng rng.Cc cy thuc chi Excoecaria l mt loi Cy g
cao 20m, ng knh 25cm. Cnh nhnh nhiu, khc khuu, v mu xm tro. Thn c
nha m. L n, mc cch, thng tp trung u cnh, phin l dy cng hnh bu dc
hoc hnh trng bu dc, u c mi li ngn, hi lch v t, gc l t, di 5 - 8cm,
rng 2,5 - 4,5cm, mp hi gn sng, gn bn 8 - 9 i, cung l mnh, di 2cm, c
mt tuyn nh gc phin l. Hoa n tnh khc gc hay cng gc. Cm hoa c bng, di
2 - 8cm. Hoa c c 3 cnh i, hnh di, 3 nh, ch nh phnh gc. Hoa ci, l bc
ging hoa c, hoa gn nh khng cung, cnh i 3, hnh tam gic. Bu hnh trng
3 , 3 vi nhy; qu nang c cung nh, c 3 cnh, ng knh 1cm. Ht hnh cu, ng
knh 4mm, mu xm nht.[3]
Hnh 1: Cy Gi (Excoecaria agallocha L.)
1.1 Tnh hnh nghin cu cy gi (Excoecaria agallocha L.)Trn th gii,
Cy Gi c nghin cu rt nhiu v thnh phn ha hc v hot tnh sinh hc. Cc cht
tch c ch yu thuc vo 2 lp cht diterpen v flavonoid.1.2 Cc
diterpenDiterpen l mt nhm cht thuc lp cht terpen, thnh phn phn t
cha 4 n v isopren (C5H8), c cng thc phn t l C20Hn, c cu to mch thng
hoc mch vng. Cc nghin cu cho bit, nha m ca cy Gi l hn hp ca cc
diterpen este (cc hp cht cha C20 ) vi cc axit bo bo ha tng t nh
axit n-cacboxylic (vi C22-C30). Cc hp cht trn c tc dng gy kch thch,
gy nhc nhi da khi tip xc.Nm 2000, Bng cc phng php ph Ammanamanchi v
ng s ca ng phn lp c 11 diterpen t dch chit n-hexan ca r ca Cy Gi
(Excoecaria agallocha L.) thu vng Godavari n . Trong c 5 diterpen
mi l: 3-oxo-ent-13epi-8(13)-epoxy-15-chloro-14-hydroxylabdan (1);
ent-15-chloro-13,14 dihydroxylabd-8(9)-en-3-on (2);
ent-15-chloro-labd-8(9)en-3,13,14-triol (3);
ent-11-hydroxy-8(14),15-isopimaradien-3-on (4);
8,13-epoxy-3-nor-2,3-seco-14-epilabden-2,4-olid (5). V 6 diterpen
bit l: ent-3-oxo-13-epi-manoyl oxid (6); ent-
3-hydroxy-13-epi-manoyl oxid (7);
(13R,14S)-ent-8,13;14,15-diepoxy-13-epi-labdan-3-on (8);
ent-16-hydroxy-3- oxo-13-epi-manoyl oxid (9);
ent-15-hydroxylabda-8(17),13E-dien-3-on (10); labda-
8(17),13E-dien-3,15-diol (11). Mt s cc Diterpen phn lp c c hot tnh
chng li s pht trin ca khi u trong c th chut [4].
Nm 2000, Tenji Konishi v cng s phn lp c 2 diterpen mi c t nha g
ca cy Gi (Excoecaria agallocha L.) thu Okinawa, Nht Bn. Cu trc ca 2
diterpen mi ny ny c xc nh nh cc phng php ph NMR v X-Ray n tinh th l
Excoecarin M (12) v Excoecarin N (13) [5].
Nm 2002, Ammanamanchi v cng s phn lp v nhn dng c 3 diterpen mi t
dch chit n-hexan ca r cy Gi (Excoecaria agallocha L.) thu vng ca
sng Godavary, n l: ent-13-epi-8,13-epoxy-
4,6-dihydroxy-3,4-secolabd-14-en-3-oat (14);
ent-13-epi-8,13-epoxy-2,3-secolabd-14-en-2,11-olid-3-oat (15);
ent-17-hydroxy-3,4-secokaura-4(19),15-dien-3-oat 3 (16) [6].
Nm 2003, Tenji Konishi v cc ng s ca ng phn lp c ba diterpen
khung secolabdan mi c tn l excoecarin S, T1 v T2 t dch chit n-hexan
ca m cy Gi (Excoecaria agallocha L.), cu trc ca cc diterpen mi ny c
xy dng da trn c s d liu quang ph 13C, 1H NMR v X-Ray n tinh th l:
13-epi-12-hydroxy-2,3-secolabda-14-en (17), axit
ent-14S,15-epoxy-2,3-seco-13-epi-labdan-2,3-dioic (18) v axit
ent-14R,15-epoxy-2,3-seco-13-epi-labdan-2,3-dioic (19). Trong T1v
T2 c cng cng thc l . V 3 diterpen bit l axit ent-12-oxo-2
,3-secobeyer-15-en-2,3-dioic (20); agallochin H (21); v
ent-15-epoxy-beyeran-3-ol (22) [7].Gn y, mt phorbol este c tn l
12-deoxyphorbol-13-(3E, 5E-decadienoat), vi c tnh chng HIV, c phn
lp t l v thn ca cy Gi (Excoecaria agallocha L.) nghin cu Ty bc
Australia. Trong qu trnh nghin cu ngi ta thy cc thnh phn diterpen c
kh nng c ch hiu qu vi virus Epstein-Barr gy ung th [1], [8].
Nm 2007, Ji-Dong Wang v cc ng s ca ng phn lp c 4 diterpen t dch
chit etyl axetat t thn cy v l ca cy Gi ( Excoecaria agallocha L.) s
dng cc phng php sc k ct s dng trn silica gel, Sephadex LH-20. Bng
cc phng php ph nh ph hng ngoi (IR), 13C v 1H NMR xc nh c cu trc ca
4 loi diterpen mi c t tn excoagallochaols A-D l: Excoagallochaol A
(23), Excoagallochaol B (24), Excoagallochaol C (25),
Excoagallochaol D(26) .Trong Excoagallochaol D(26) l ng phn ca
Excoagallochaol C (25) [8].
Nm 2009, Zhan Chang Wang v cng s phn lp t dch chit etanol ca v
cy Gi (Excoecaria agallocha L.) c 4 diterpen trong mt diterpen mi v
ba diterpen bit. l ent-16-hydroxy-atisan-3,4-lacton (30);
ent-16-hydroxy-atisan-3-on (27); ent-atisan-3,16-diol (28); axt
ent-3,4-seco-16-hydroxyatis- 4(19)-en -3-oic (29) [9].Mt s diterpen
phn lp t g ca cy Gi (Excoecaria agallocha L.) cho thy hot tnh chng
khi u cng nh ngn chn s chng g ca mt s loi sinh vt nh s, giun ng,
rong bin [4], [9].
Nm 2010, Yongxin Li v cng s phn lp t dch chit metanol ca thn v
cnh ca cy Gi (Excoecaria agallocha L.) Trung Quc. T phn lp c su
diterpen khung ent-kauran c tn l agallochaol K-P l : Agallochaol K
(axt ent-17-hydroxy-3,4-secokaur-4(18),15-die-3-oic ) (31) ;
Agallochaol L (axt
ent-17-caffeoyloxy-3,4-secokaur-4(18),15-die-3-oic ) (32);
Agallochaol M (axt
ent-17-coumaroyloxy-3,4-secokaur-4(18),15-die-3-oic ) (33);
Agallochaol N (axt ent-3,4-secokaur-4(18),15-dien-3-oic ) (34);
Agallochaol O (ent-17-caffeoyloxykaur-15-en-3-on) (35); Agallochaol
P (ent-kaur-15-en-3b,17-diol-2-on) (36); Agallochaol M (axt
ent-3,4-secoatisan-4(18),16-dien-3-oic) (37). V c 9 diterpen bit l:
agallochin O (38); ent-17-hydroxykaur-15-en-3-on (39);
ent-kaur-15-en-3,17-diol (40); 7-deoxogeayin (41);
ent-15-hydroxylabd-8(17),13E-dien-3-on (42);
ent-15,18-dihydroxylabd-8(17),13E-dien (43); ent-3,
11-dihydroxyisopimara-8(14),15-dien-2-on (44); v
ent-3-hydroxybeyer-15-en-2,12-dion (45).Trong cc diterpen
Agallochaol K (axt ent-17-hydroxy-3,4-secokaur-4(18),15-die-3-oic )
(31), Agallochaol O (ent-17-caffeoyloxykaur-15-en-3-on) (35),
Agallochaol M (axt ent-3,4-secoatisan-4(18),16-dien-3-oic) (37),
ent-17-hydroxykaur-15-en-3-on (39), ent-kaur-15-en-3,17-diol (40),
v ent-15,18-dihydroxylabd-8(17),13E-dien (43) cho thy tim nng chng
vim hiu qu [10].
1.3 Cc flavonoid Flavonoid l cc hp cht polyphenol, a dng v cu
trc ha hc v sinh hc. Chng c hu ht cc b phn ca cy, c bit l trong cc
t bo thc vt quang hp. Cc flavonoid khng c tng hp ngi v ng vt.Nm
2010, Yusnita Rifai v cc ng s ca ng phn lp c t dch chit metanol, n-
hexan, etylaxetat v butanol t l ca cy Gi (Excoecaria agallocha L.)
8 flavonoid. Cu trc ca cc cht c xy dng nh s dng cc phng php ph,
trong c 2 flavonoid mi (45), (46) v 6 flavonoid glycosid bit:
afzelin (47); quercitrin (48); rutin (49);
kaempferol-3-O-(2-O-acetyl--L-rhamnopyranosid (50); kaempferid 3
O--L-rhamnopyranosid (51) v kaempferol 3-Oa-Larabinofuranosid
(52).Ba glycoside flavonoid bao gm 2 hp cht mi (45 v 46) c phn lp t
cy Gi (Excoecaria agallocha L.) l cht c ch v chng li t bo ung th
tuyn ty ca ngi v t bo ung th tuyn tin lit mang li nhiu hiu qu tch
cc [11].
Nm 2011, Yongxin Li v cc ng s ca ng phn lp c t dch chit butanol
ca cy Gi (Excoecaria agallocha L.). Bng cc phng php phn tch trn
quang ph IR, MS, NMR xc nh c cy trc ca bn flavonoid mi. Bn
flavonoid mi c phn lp l: Excoecariphenol A (53); Excoecariphenol B
(54) ; Excoecariphenol C (55) ; Excoecariphenol D (56). Nhng
flavonoid mi ny c th nghim v cho kt qu tch cc trong phng chng bnh
vim gan HCV [12].
2. Hot tnh sinh hc ca cy gi (Excoecaria agallocha L.)Cy Gi
Excoecaria agallocha c hot ng tt nh cha nhng thnh phn ha hc, c bit
l:0. Diterpen0. Triterpen0. V nhng flavonloid
Mt s c tnh tr liu ca cy Gi:
c s dng theo truyn thng cha tr:0. Nhng vt thng 0. Nhng vt chch
ca mt s loi ng vt bin0. Nhng bnh gy l lot0. c xem nh mt thuc ty x v
lm nn
R cy Gi c th cha tr c au rang v sung.Nc p ca l cy Gi un si trong
du c p dng trong:0. Nhng bnh thp khp0. Bnh phong ci0. Bnh bi litNc
nu sc ca l cy Gi c dung trong cha bnh ng kinh v nhng bnh l lot. V
cy Gi l cht ty x v lm nn. Du t v cy Gi c hiu qu chng nhng bnh thp
khp, bnh phong ci v bnh t lit.
II. KT QU NGHIN CU
Thc nghim mu l cy Gi:
S 1: Quy trnh chit mu thc vt
Mu l kh 5.7kg1. Chit vi MeOH 95%2. Ct loi MeOHCn MeOH530gThm
2lit H2OChit vi ethyl axetat ct loi dmCn chit EtOAc 54.5gDch nc cn
li310gChit vi n-hexanct loi dmCn chit n-hexan 96.1g
S 2: Quy trnh phn lp cht t cn n-hexan ca l cy Gi50g cn
n-hexan
Sc k ct silicagel (10/1)n-hexan/EtOAc5% EtOAc10% EtOAcP2
12.4g
P1 6.6g
20%-50% EtOAcI.1I.2I.3P3-P7
EA2 (3) (taraxerol)
EA3 (2)(epitaraxerol)EA1 (1)(taraxerone)
5.7kg bt l kh ca cy Gi c chit siu m trong MeOH 95% 3ln (3
ting/ln). Dch chit tng c c quay p sut gim thu c 530g cn tng MeOH.
em pha long cn tng MeOH vi 2lit nc v chit phn b ln lt vi n-hexan v
etyl axetat, c quay gim p thu c 96.1g cn n-hexan, 54.5g cn etyl
axetat v 310g cn nc. Tin hnh phn lp cht t 50g cn n-hexan ca dch
chit tng MeOH t l ca cy gi bng sc k ct silica gel (t l cht/silica
gel=10/1) vi h dung mi n-hexan/etyl axetat, tng dn t l etylaxetat t
0%-100% thu c 7 phn on chnh.Phn on 1(6.6g) thu c khi ra gi bng h
n-hexan/etyl axetat vi t l etyl axetat 5%. Phn on 1 c tinh ch bng
sc k ct (t l cht/silica gel=1/25), vi h dung mi ra gii l
n-hexan/etyl axetat = 65/1 thu c 3 phn on chnh I.1, I.2, I.3. Kt
tinh phn on I.1 trong n-hexan, ra li bng n-hexan lnh thu c 0.15g
cht EA1(taraxer-14-en-3-one) l tinh th hnh kim, mu trng, Rf= 0.52
(n-hexan/etylaxetat = 20/1), tonc= 241-243oC. Phn on I.3 c kt tinh
li trong n-hexan thu c 96mg EA3 (epitaraxerol) l tinh th hnh kim,
mu trng, Rf=0.33 (n-hexan/etylaxetat = 20/1), tonc= 268-269oC.Phn
on 2(12.4g) thu c khi ra gi bng h n-hexan/etyl axetat vi t l etyl
axetat 10%. Sc k ct phn on 2 (t l cht/silica gel=30/1), dung mi ra
gii l n-hexan/etylaxetat=50/1 thu c 1phn on chnh. Kt tinh trong
CH2Cl2 v ra li bng CH2Cl2 lnh thu c 0.60g cht EA2 (taraxerol), dng
tinh th hnh phin mu trng, Rf= 0.61 (n-hexan/etylaxetat = 10/1),
tonc= 279-280oC.* Tho lun kt qu
Hp cht EA1 (1): 14-taraxerene-3-oneHp cht EA1 thu c t cn chit
n-hexan ca dch chit tng metanol ca loi Excoecaria agallocha L. l
cht kt tinh hnh kim, mu trng. Ph 1H-NMR c dng ph ca mt hp cht
triterpen trong c s xut hin ca 8 nhm metyl bc bn trong phn t th hin
qua 8 tn hiu singlet ti H 0.83 (3H), 0.91 (3H), 0.92 (3H), 0.95
(3H), 1.06 (3H), 1.08 (3H), 1.09 (3H), v 1.14 (3H). Ngoi ra, trn ph
cn thy xut hin tn hiu mt proton olefin ti H 5.55 (1H, dd, J = 8.0,
3.5 Hz). Ph 13C-NMR xut hin tn hiu ca 30 cacbon, da vo kt qu ca cc
ph DEPT cho thy hp cht ny c 8 cacbon bc bn ti C 217.5, 157.6, 47.5,
38.8, 37.7, 37.5, 35.7, 28.7; 4 nhm metin ti C 117.1, 55.7, 48.8,
48.7; 10 nhm metylen ti C 40.6, 38.3, 37.6, 36.6, 35.1, 34.1, 33.5,
33.1, 19.9, 17.4; v 8 nhm metyl ti C 33.3, 29.9, 29.8, 26.1, 25.5,
21.4, 21.3 v 14.8. Trong tn hiu ca nhm cacbonyl c xc nh ti C 217.5,
mt ni i b th 3 v tr ti C 157.6 (C) v 117.1 (CH). Trn ph ESI-MS ion
dng cht EA1 cho pic ion phn t ti m/z = 425 [M + H]+. Cng thc phn t
c xc nh thng qua cc d liu ph MS v 13C-NMR l C30H48O. Phn tch cc tn
hiu proton ca cc nhm metyl trn ph 1H-NMR v chuyn dch ha hc ca cc
nguyn t cacbon trn ph 13C-NMR khng nh EA1 l mt triterpen khung
taraxeran. So snh d liu ph ca EA1 vi ti liu [] cho php kt lun EA1
chnh l 14-taraxeren-3-one. Hp cht EA3 (2): 14-taraxerene-3-ol
(epitaraxerol)Hp cht EA3 thu c di dng tinh th mu trng. Trn ph
ESI-MS ion dng cht EA3 cho pic ion phn t ti m/z = 427 [M + H]+. Cng
thc phn t c xc nh thng qua cc d liu ph MS v 13C-NMR l C30H50O. Trn
ph 1H-NMR ca EA3 xut hin tn hiu ca 8 nhm metyl bc bn ti H 0.82,
0.86, 0.91(6H), 0.94(9H), 1.09 di dng cc singlet, mt tn hiu proton
ni i ti H 5.52 (dd, J = 8.0, 3.5Hz) v mt tn hiu proton ti H 3.40
(1H, br). Nhn chung, ph 1H-NMR ca EA3 kh ging vi hp cht EA1, trn ph
ca EA3 c xut hin thm tn hiu proton ti H 3.40 y l tn hiu ca nhm
oximetin. iu ny cho php d on EA3 l mt dn xut b kh nhm cacbonyl
(>C=O) ca EA1. Ph 13C-NMR ca EA3 xut hin tn hiu ca 30 cacbon kh
ging vi hp cht EA1. Tuy nhin, trn ph 13C-NMR ca EA3 khng thy xut
hin tn hiu ca nhm cacbonyl (C 217.5) m thay vo l tn hiu ca nhm
oxymethine ti C 76.2. iu ny mt ln na khng nh d on trn l ph hp. Phn
tch cc d kin ph thu c v so snh vi ti liu cng b [], hp cht EA3 c xc
nh l epitaraxerol, mt dn xut ca taraxerone.Hp cht EA2 (3):
14-taraxerene-3-ol (taraxerol)Hp cht EA2 thu c di dng tinh th hnh
phin mu trng, c nhit nng chy tonc= 279-280oC. Ph 1H-NMR ca EA2 xut
hin 8 tn hiu singlet ca 8 nhm metyl bc bn ti H 0.80 (3H), 0.82(3H),
0.91(6H), 0.93(3H), 0.95(3H), 0.97(3H), 1.09(3H), mt tn hiu proton
ni i ti H 5.54(dd, J = 8.0, 3.5 Hz), v mt tn hiu proton ti H 3.19
(1H, m). Ph 13C-NMR ca EA2 xut hin tn hiu ca 30 nguyn t. Cc ph 1H-
v 13C-NMR ca EA2 v EA3 hon ton tng ng vi nhau. Ch c mt im khc bit v
chuyn dch proton v cacbon ca nhm oximethine l: tn hiu proton ca nhm
oximethine trong EA2 (H 3.19) chuyn dch v pha trng mnh hn trong EA3
(H 3.40), cn tn hiu cacbon ca nhm oxymethine trong EA2 (C 78.8)
chuyn dch v pha trng thp hn trong EA3 (C 76.2) iu ny cho php d on
EA2 l ng phn ca EA3 vi nhm oxymethine nm v tr 3. Phn tch cc d kin
ph thu c v so snh vi ti liu cng b [], hp cht EA2 c xc nh l
taraxerol. Nh vy khi nhm cacbonyl (>C=O) trong EA1 b kh ha s thu
c hai dng ng phn 14-taraxerene-3-ol (EA3) v 14-taraxerene-3-ol
(EA2). y l ln u tin hp cht ny c phn lp t loi E.Agalocha. Khi nghin
cu kh nng c ch enzym acetylcholinesterase (AchE) t mt s loi thc vt
v nm, Peter J. Houghton v cng s pht hin c kh nng c ch AchE ca
14-taraxeren-3-ol vi gi tr IC50 l 79M [63].
V trTaraxerone3-taraxerol3-taraxerol
23 41.08s0.93s0.94s
24 41.06s0.80 s0.82s
25 101.09s0.91s0.94s
26 81.14s0.97s1.09s
27 130.92s1.09s0.91s
28 170.83s0.82s0.86s
29 200.91s0.91s0.91s
30 200.95s0.95s0.93s
Hp cht EA1, EA2, EA3V tr CEA1 (1)14-taraxeren-3-on[TL1,2]EA2
(3)14-taraxeren-3-ol[TL2]EA3 (2)14-taraxeren-3-ol(epitaraxerol)
[TL3]
C (CDCl3, 125MHz)H (CDCl3, 500MHz)C (CDCl3, 125MHz)H (CDCl3,
500MHz)C (CDCl3, 125MHz)H (CDCl3, 500MHz)
138.3(CH2)37.637.7(CH2)
234.1(CH2)2.56 (1H, ddd, J=16.0, 7.6, 3.5 Hz, Ha-2), 2.32(1H,
ddd, J=16.0, 6.5, 3.5 Hz, Hb-2)26.725.0(CH2)
3217.5(C)78.83.19 (m)76.2(CH)3.40(br)
447.5(C)38.839.1(C)-
555.7(CH)55.449.2(CH)
619.9(CH2)18.618.7(CH2)
735.1(CH2)35.033.7(CH2)
838.8(C)38.638.0(C)
948.7(CH)48.648.9(CH)
1035.7(C)37.437.5(C)
1117.4(CH2)17.317.4(CH2)
1237.6(CH2)37.535.1(CH2)
1337.7(C)35.637.3(C)
14157.6(C)158.0158.2(C)
15117.1(CH)5.55(dd, J=8.0, 3.5, H-15) 116.75.54(dd, J=8.0,
3.5)116.7(CH)5.52(dd, J=8.0, 3.5)
1636.6(CH2)36.536.7(CH2)
1737.5(C)37.835.8(C)
1848.8(CH)49.548.7(CH)
1940.6(CH2)41.241.2(CH2)
2028.7(C)28.628.8(C)
2133.5(CH2)33.533.1(CH2)
2233.1(CH2)32.932.2(CH2)
2326.1(CH3)1.08s 27.70.93s 33.3(CH3)0.94s
2421.3(CH3)1.06s 15.30.80s 22.1(CH3)0.82s
2514.8(CH3)1.09s 15.20.91s 29.9(CH3)0.94s
2629.8(CH3)1.14s 29.6 0.97s 26.0(CH3)1.09s
2725.5(CH3)0.92s 25.7 1.09s 29.8(CH3)0.91s
2829.9(CH3)0.83s 29.7 0.82s 21.2(CH3)0.86s
2933.3(CH3)0.91s 33.1 0.91s 28.2(CH3)0.91s
3021.4(CH3)0.95s 21.1 0.95s 15.2(CH3)0.93s
KT LUNTrong qu trnh nghin cu ny, chng ti thu c mt s kt qu
sau:10. x l mu cy Gi to cao chit tng v cao chit phn on bng cc dung
mi hu c khc nhau.1. Bng vic kt hp cc phng php sc k phn lp c 3 hp
cht t cao chit n-hexan ca cy Gi, kt hp vi cc phng php ph hin i bao
gm cng hng t ht nhn mt chiu (1H-NMR, 13C-NMR, DEPT), ph khi lng
(ESI-MS) phn lp c cu trc ha hc ca 3 hp cht. Cc hp cht l:0. Hp cht
EA1 (1): 14-taraxerene-3-one0. Hp cht EA3 (2): 14-taraxerene-3-ol
(epitaraxerol)0. Hp cht EA2 (3): 14-taraxerene-3-ol (taraxerol)
TI LIU NGHIN CU1. Tuyn tp bo co Hi ngh Sinh thi v Ti nguyn sinh
vt ln th 3, 22/10/2009 - Vin ST&TNSV - Vin KH&VN Vit Nam.2.
L nh Mi, Dng c Huyn, Nguyn Tin Bn (2004), Chi n ta (Excoecaria L.),
chi Lnh cng (Fissistigma Griff.) Ti nguyn thc vt ng Nam , Nh xut bn
Nng nghip, H Ni, tp 9(6), trang 3-18.3. Phm Hong H,Cy c Vit Nam,
quyn 2 trang 284.4. Ammanamanchi S.R. Anjaneyulu , Vadali Lakshmana
Rao, (2000),Phytochemistry, V. 55, p. 891-893.5. Tenji Konishi,
Takao Konoshima, Takashi Maoka and Yasuhiro Fujiwara, (2000),
Tetrahedron Letters, V.41, p. 3419.6. Ammanamanchi S.R. Anjaneyulu,
Vadali Lakshmana Rao, (2002), Phytochemistry, V.62, p. 585-586.7.
Tenji Konishi, Takao Konoshima, Takashi Maoka and Yasuhiro
Fujiwara, (2003), Phytochemistry, V.64, p. 835- 840.8. Ji-Dong Wang
, Wen Zhang , Zhen-Yu Li , Wen-Sheng Xiang ,Yue-Wei Guo , Karsten
Krohn, (2007), Phytochemistry, V.68, p. 2436-2440.9. Zhan Chang
Wang , Yi Ming Lin , Dan Qin Feng , Cai Huan Ke , Peng Lin , Chong
Ling Yan and Jun De Chen, (2009), Open Access, V.14, p.414- 420.10.
Yongxin Li, Jun Liu, Shanjiang Yu , Peter Proksch, Jun Gu, Wenhan
Lin, Phytochemistry, (2010), V.71, p. 2124- 2130.11. Yusnita Rifai,
Midori A. Arai, Samir K. Sadhu, Firoj Ahmed, Masami Ishibashi,
(2011), Bioorganic & Medicinal Chemistry Letters V.21, p.718-
722.12. Yongxin Li, Shanjiang Yu, Dong Liu, Peter Proksch, Wenhan
Lin, (2011), Bioorganic & Medicinal Chemistry Letters ,
p.1-313. Nguyn nh Triu, Cc phng php vt l hin i ng dng trong ha hc,
Nh xut bn i hc quc gia H Ni, 2012.
PH LC