Non Homologous End Joining. Homologous Recombination Non Homologous End Joining.

Non Homologous End Joining

Homologous Recombination

Non Homologous End Joining

Differences between HR and NHEJ:

• Error- free / Error- prone• Cell cycle regulated • Varies between species

HR NHEJ

yeast mammalsplants

HR NHEJ

MHC rearrangementMeiosis

Cell-induced Double Strand Brakes

V(D)J recombination

NHEJ Defects

• SCID (Severe Combined Immune Deficiency).

• Cancer – genome instability: rearrangements, deletions, amplifications.

Ku 70/80 heterodimer

Ku has a role in telomeres

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KU 70/80

Ku is critical for telomere maintenance

Ku Plays Several Roles at Telomeres

Telomere addition

Telomeric silencing

Ku is a multifunctional protein

HR

NHEJ