WT nNOS -/- iNOS -/- eNOS -/- n/i/eNOS -/- 0 20 40 60 80 100 Relaxation (%) 0 20 40 60 80 100 Relaxation (%) 0 20 40 60 80 100 Relaxation (%) 0.5 0.4 0.3 0.2 0.1 0 Contraction (g) 0.6 0.7 9 8 7 5 6 9 8 7 5 6 9 8 7 5 6 9 8 7 5 6 Acetylcholine (-log mol/L) Forskolin (-log mol/L) DEA-NONOate (-log mol/L) Phenylephrine (-log mol/L) A B C D Figure I in the Online Data Supplement
nNOS -/-
Jan 03, 2016
WT. nNOS -/-. iNOS -/-. eNOS -/-. n/i/eNOS -/-. B. A. 0. 0. 20. 20. 40. 40. Relaxation (%). Relaxation (%). 60. 60. 80. 80. 100. 100. *. *. 7. 6. 5. 9. 8. 6. 9. 8. 7. 5. Forskolin (-log mol/L). DEA-NONOate (-log mol/L). C. D. 0.7. *. *. *. *. 0.6. 0. 0.5. - PowerPoint PPT Presentation
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WT nNOS-/- iNOS-/- eNOS-/- n/i/eNOS-/-
0
20
40
60
80
100
Re
laxa
tion
(%
)
0
20
40
60
80
100
Re
laxa
tion
(%
)
0
20
40
60
80
100R
ela
xatio
n (
%)
0.5
0.4
0.3
0.2
0.1
0
Co
ntr
act
ion
(g
)
0.6
0.7
9 8 7 56 9 8 7 56
9 8 7 56 9 8 7 56
Acetylcholine (-log mol/L)
Forskolin (-log mol/L) DEA-NONOate (-log mol/L)
Phenylephrine (-log mol/L)
A B
C D
Figure I in the Online Data Supplement
Figure II in the Online Data Supplement
WT nNOS-/- iNOS-/- eNOS-/- n/i/eNOS-/-0
10
20
30
40P
lasm
a re
nin
activ
ity (
ng/
mL/
hr)
*
0
100
200
300
400
500
WT nNOS-/- iNOS-/- eNOS-/- n/i/eNOS-/-
Pla
sma
aldo
ster
one
(pg/
mL)
*
A
B
Pe
ri-re
na
l wh
ite a
dip
ose
tis
sue
we
igh
t （
g）
C57BL/6J 129SV
C57BL/6J 129SVC57BL/6J 129SV
0
0.1
0.2
0.3E
pid
idym
al w
hite
ad
ipo
se
tissu
e w
eig
ht （
g）
Sys
tolic
blo
od
pre
ssu
re(m
mH
g)
0
20
40
6080
100
120
140
160
Dia
sto
lic b
loo
d p
ress
ure
(mm
Hg
)
0
20
40
6080
100
120
140
160
B
A D
E
FC
0
0.10
0.12
0.06
0.08
0.02
0.04
C57BL/6J 129SV
NS NS
NS NS
0
2
4
6
8
10
Dia
me
ter
of
ep
idid
yma
l w
hite
ad
ipo
se c
ells
(m
)
0
20
40
6080
100
120
140
160
Me
an
blo
od
pre
ssu
re(m
mH
g)
C57BL/6J 129SV
C57BL/6J 129SV
C57BL/6J 129SV
NS
NS
Figure III A-F in the Online Data Supplement
Pla
sma
LD
L
(mm
ol/L
)
Pla
sma
sm
all
de
nse
LD
L
(mm
ol/L
)
G I
H J
K
Pla
sma
ad
ipo
ne
ctin
(g
/mL
)
Pla
sma
trig
lyce
ride
(mm
ol/L
)
0.5
1.0
1.5
0
2.0
C57BL/6J 129SV
NS
Time (minutes)
Pla
sma
glu
cose
(m
mo
l/L)
0 30 60 90 120 0 30 60 90 120
C57BL/6J 129SV
0
5
10
15
20
25
30
0
5
10
15
20
25
30
0
0.5
0.3
0.4
0.2
0.6
0.1
C57BL/6J 129SV
NS
C57BL/6J 129SV
0
0.8
1.0
0.4
0.6
0.2
0
5
10
15
20
25
C57BL/6J 129SV
NS NS
Figure III G-K in the Online Data Supplement
Pla
sma
glu
cose
(m
mo
l/L)
129SV
C57BL/6J
LLarge epicardial coronary arteries
Coronary microvessels
129SV
0Neo
intim
al f
orm
atio
n
0.05
0.10
0.15
129SVC57BL/6J0
Me
dia
l thi
cke
nin
g
0.1
0.2
0.3
0.4
0.5
129SVC57BL/6J
Per
iva
scul
ar
fibro
sis
0
0.1
0.2
0.3
0.4
0.5
129SVC57BL/6J
0Neo
intim
al f
orm
atio
n
0.05
0.10
0.15
129SVC57BL/6J0
Me
dia
l thi
cke
nin
g0.1
0.2
0.3
0.4
0.5
129SVC57BL/6J
Per
iva
scul
ar
fibro
sis
0
0.1
0.2
0.3
0.4
0.5
129SVC57BL/6J
C57BL/6J NS NSNS
NS NSNS
Figure III L in the Online Data Supplement
Actin
iNOS
Actin
iNOS
*
Actin
iNO
S /
act
in
1.0
0
0.2
0.4
0.6
0.8
nNO
S /
act
in
1.0
0
0.2
0.4
0.6
0.8
eNO
S /
act
in
1.0
0
0.2
0.4
0.6
0.8
n/i/eNOS-/-WT nNOS-/- iNOS-/- eNOS-/-
n/i/eNOS-/-WT nNOS-/- iNOS-/- eNOS-/-
n/i/eNOS-/-WT nNOS-/- iNOS-/- eNOS-/-
iNO
S /
act
in
1.0
0
0.2
0.4
0.6
0.8
nNO
S /
act
in
1.0
0
0.2
0.4
0.6
0.8
eNO
S /
act
in
1.0
0
0.2
0.4
0.6
0.8
n/i/eNOS-/-WT nNOS-/- iNOS-/- eNOS-/-
n/i/eNOS-/-WT nNOS-/- iNOS-/- eNOS-/-
n/i/eNOS-/-WT nNOS-/- iNOS-/- eNOS-/-
Actin
eNOS
Actin
eNOS
Actin
nNOS
Actin
nNOS
1 month of age 6 months of ageA D
1 month of age 6 months of ageB E
1 month of age 6 months of ageC F
Figure IV in the Online Data Supplement
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