Distribution of Xanthine Dehydrogenase and Xanthine Oxidase in milk fractions : Evidence for post- transcriptional regulation of Xanthine Oxidase in the frame of the mammary innate immune defense system Nissim Silanikove, Fira Shapiro, Adi Rauch & Gabriel Leitner
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Nissim Silanikove, Fira Shapiro, Adi Rauch & Gabriel Leitner
Distribution of Xanthine Dehydrogenase and Xanthine Oxidase in milk fractions : Evidence for post-transcriptional regulation of Xanthine Oxidase in the frame of the mammary innate immune defense system. Nissim Silanikove, Fira Shapiro, Adi Rauch & Gabriel Leitner. Nitrite NADH. NitrateNAD. - PowerPoint PPT Presentation
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Distribution of Xanthine Dehydrogenase and Xanthine Oxidase in milk fractions :
Evidence for post-transcriptional regulation of Xanthine Oxidase in the
frame of the mammary innate immune defense system
Nissim Silanikove, Fira Shapiro, Adi Rauch & Gabriel Leitner
Or 2H2O2
NitrateNAD
Nitrite
NADH
SOD
Nitric Oxide is a Free RadicalN=O
Reaction of Lactoperoxidase with Hydrogen peroxide and
Nitrite
1 .LPO + H2O2 LPO compound 1
2. LPO compound I + NO2- LPO compound II + ●NO2
3 .LPO compound II + NO2- LPO + ●NO2
Scenario of NO cycling and metabolism in mammary secretion (Free radicals Biol Med, 2005)
Question Number 1
1. In the mammary gland, XOR has an essential, non-enzymatic, structural role in fat secretion (Vorbach et al. Genes Dev 2002, 16:3223)
2. It is well established that XOR associated with fat secretion is located within the inner side of MFGM ) e.g. J. Physiol 2002, 545:567)
Do we have sufficient XO to support its role in innate immunity? (Free radicals biol Med
2005, 38: 1139 )
Xanthine + hypoxanthine and uric acid concentration in oxytocin-induced and
mature milk
0
5
10
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Oxytocin Induced Mature milk
Mic
ro-m
ola
r
Xanthin+HypoxanthinUric Acid
Question Number 2
As mature fresh milk do not contains measurable amount of xanthine, but contains uric acid in the range of 30 40 -micro-molar, it is important to know whether it derived from milk xanthine or secreted as uric acid?
Fresh milk (i.e., milk secreted into the alveoli within 5 to 10 min before sampling) was obtained at the end of noon-milking following injection of oxytocine.
Distribution of xanthine oxidase, alkaline phosphatase and acid phosphatase in milk fractions