NIIH Examination Date Notice The NIIH Examination Date has been finalised for 19 th March 2019. The time for examination will be 9:00 am to 11:00 am. The examination will be conducted at iDZ Powai Near Ramada Hotel Mumbai. The Hall tickets is released by mahaonline.gov.in Please Note that the exam related schedule and rules are under control of NIIH and they preserve the rights to change/modify the same as per circumstances faced.
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NIIH Examination Date Notice
The NIIH Examination Date has been finalised for 19th March 2019.
The time for examination will be 9:00 am to 11:00 am. The
examination will be conducted at iDZ Powai Near Ramada Hotel
Mumbai. The Hall tickets is released by mahaonline.gov.in
Please Note that the exam related schedule and rules are under control of NIIH and they preserve
the rights to change/modify the same as per circumstances faced.
National Institute of Immunohaematology Syllabus Document
Post Name - Technical Assistant
Syllabus: Life Science/ Microbiology/Immunology/ Biotechnology/ Biochemistry:
Immunology (20 Questions)
Emphasis on principal and uses/application ,Immunity –Basic principles and classification, Antigen,
General Characters of viruses, Classification in brief and name of the diseases they produce.,
Hepatitis viruses, HIV, (Polio, Rabies, Rata, Measles, Dengue) - Oncogenic viruses in brief, Collection
and transport of virological specimens - Laboratory diagnosis of viral infections (various methods of
virus culture, serology etc.)
Widal test and preparation of Salmonella antigens, VDRI Test, Latest agglutination tests for (RA, CRP,
ASO, Pregnancy Test, Australia Antigen, Toxoplasmosis) ELISA test RIA Test, Get diffusion techniques
and ,Immuno electro phoresis, Detection of Antigen / Antibody for Malarial (ICT), Optimal Test,
Assay of immunoglobulins
Biotechnology (20 Questions)
1. Bioinformatics: Introduction to Bioinformatics, Scope of bioinformatics, bioinformatics and the internet, useful bioinformatics and sites on the www, data explosion, sequencing DNA,RNA and proteins
2. Centrifugation: Principle of centrifugation. Rotor design and selection. Preparative centrifugation - differential, rate-zonal, isopycnic, equilibrium isodensity centrifugation with applications. Density gradient centrifugation – nature of gradient, formation, sample
3. Application and collection. Column Chromatography: Principle, working and applications of GC, affinity, ion exchange, gel permeation, HPLC
4. Details of enzymes involved in gene cloning and their mode of action: Restriction endonucleases - types, nomenclature, target sites, nature of cut ends, host control restriction and modification, star activity, isoschizomers, application of RE
5. Application of recombinant DNA technology: Diagnosis of genetic diseases – Sickle cell anaemia; Gene therapy – somatic and germ line gene therapy, Commercial products – insulin
7. Types of mutations: Point mutation, reverse mutation, suppressor mutation, frameshift mutation, conditional lethal mutation, base pair substitution, transition, transversion, missense mutation, nonsense mutation, silent mutation, neutral mutation, pleiotropic mutations.
8. Transcription - mechanism of RNA transcription: Initiation, elongation and termination; processing of t RNA, r RNA, mRNA (prokaryotes and eukaryotes)- concept of split genes, reverse transcription. Role of Inhibitor- Rifampicin , Actinomycin D
9. Translation (protein biosynthesis)– Genetic code, mechanism of translation: Activation of amino acids, chain initiation, elongation & termination: Post translational modifications of proteins
Biochemistry (20 Questions)
Fundamentals of analytical bio-chemistry and instrumentation. 1. Clinical enzymology – Diagnostic enzymes, Iso-zymes. 2. Fundamentals of Analytical Bio-chemistry & Instrumentation - Analytical balance - Centrifuges - Colorimeter and spectrophotometer - Flame photometer - Auto analyzers - Chromatography - Electrophoresis Chromatography Principle, Technique and Applications of the following kinds of chromatography :
Partition chromatography ( Paper), Adsorption Chromatography (TLC and column); Ion exchange
chromatography and Gel filtration Principles of GLC, HPLC, Affinity chromatography
1. pH and BufferspH meter and Glass electrode
2. Blood related diseases Iron deficiency anemia. Sickle cell anemia. Thalassemia
3. Amino acid and protein metabolism
4. Electrophoresis -Principle ; Factors affecting the rate of migration of sample in an electric field Supporting media – paper, cellulose acetate, agar, agarose and polyacrylamide Discontinuous electrophoresis – Native PAGE Applications of electrophoresis - Separation of proteins and Nucleic acids with one staining method for each; molecular weight determination
Life Science (20 Questions) 1. Carbohydrates - Catabolism 1. Glycolysis – process and metabolic regulation 2. Citric Acid Cycle –
a) Process and regulation. b) Importance as a central amphibolic pathway unifying all primary biological processes.
2. Leukocytes: Total count, differential count and variation in number, types of leukocytes and function, Differential count, Leucopoiesis and Leukemia. Blood coagulation and lymph: Formation of blood platlets (Thrombopoiesis), clotting mechanism, Bleeding and clotting time, anticoagulants, failure of clotting mechanism.
3. Hybridoma technology: Somatic cell fusion, somatic cell genetics, production & applications of monoclonal antibodies
4. DNA finger printing: PCR, Southern blotting, RFLP; application in forensic sciences, parenthood testing etc.
5. Normal human karyotype: Karyotype preperation& banding techniques, band numbering scheme, human genome sequencing & human genome project.
Microbiology (20 Questions)
History of Microbiology, Microbes and their classification , Study of different , microscopes,
Morphology of bacteria, Motional requirements of bacteria, Preparation and uses of culture media,
Culture methods and identification of bacteria Sterilization and Disinfection
Post Name – Technician C
Syllabus: Life Science/ Microbiology/Immunology/ Biotechnology/ Biochemistry:
Immunology (20 Questions)
Immunity –Basic principles and classification, Antigen, Antibody (Immunoglobulin’s), Complement
system,
General Characters of viruses, Classification in brief and name of the diseases they produce.,
Hepatitis viruses, HIV, (Polio, Rabies, Rata, Measles, Dengue) - Oncogenic viruses in brief, Collection
and transport of virological specimens - Laboratory diagnosis of viral infections (various methods of
virus culture, serology etc.)
Widal test and preparation of Salmonella antigens, VDRI Test, Latest agglutination tests for (RA, CRP,
ASO, Pregnancy Test, Australia Antigen, Toxoplasmosis) ELISA test RIA Test, Get diffusion techniques
and Immuno electro phoresis, Detection of Antigen / Antibody for Malarial (ICT), Optimal Test, Assay
1. Antigens and Antibodies: Properties of antigen, adjuvant, epitopes. Basic structure classes &
sub- classes of antibodies with their biological activity, complement, antigenic determinants on immunoglobulins.
2. Basic & cellular immunology : Types of immunity & immune response, Mechanisms of acute
inflammation, lymphoid organism structure and classes antibodies, antigen and antibody
reactions, complement system, histocompatibility antigens (MHC) and their role in antigen presentation & cytotoxicity, cell adhesion molecules & their role in immunity
3. Hypersensitivity reactions
4. Autoimmunity 5. History of Microbiology, Microbes and their classification , Study of different , microscopes,
Morphology of bacteria, Motional requirements of bacteria, Preparation and uses of culture
media, Culture methods and identification of bacteria Sterilization and Disinfection
6) Molecular Biology (10 Questions)
1. DNA/RNA structure, transcription, translation, genomics, proteomics,stem cells 2. Sequencing Techniques, Basic Principles and Instrumentation, working and applications of- •
Purification of Proteins/ Enzymes • Protein Sequencing Techniques • DNA Sequencing
1. Antigens and Antibodies: Properties of antigen, adjuvant, epitopes. Basic structure classes &
sub- classes of antibodies with their biological activity, complement, antigenic determinants
on immunoglobulins.
2. Basic & cellular immunology : Types of immunity & immune response, Mechanisms of acute inflammation, lymphoid organism structure and classes antibodies, antigen and antibody
reactions, complement system, histocompatibility antigens (MHC) and their role in antigen
presentation & cytotoxicity, cell adhesion molecules & their role in immunity 3. Hypersensitivity reactions
4. Autoimmunity
5. Immunology of transplantation
6. Immune hemolyticanemia, Immune thrombocytopenia , Immune neutropenia 7. Immunodeficiency diseases a) Primary Immunodeficiency b) Defects in the Complement
System c) Treatment Approaches for Immunodeficiency d) Animal Models of Primary
Immunodeficiency e) Secondary Immunodeficiency & AIDS
7) Epidemiology (8 Questions)
1. Principles of Epidemiology and Epidemiological Methods
2. Epidemiology of hematological disorders
3. Health programmes related to hematological disorders in India
4. Genetics and health
5. Basic medical statistics
8) MBBS general topics (13 Questions)
1) Microbiology-Microbes and their classification , Morphology of bacteria, Motional
requirements of bacteria, uses of culture media, Culture methods , Sterilization and
Disinfection
2) General pathology of hematological and thrombotic disorders
3) General pathology of infectious diseases including Hepatitis B ,C and HIV
Post Name – Scientist B (Bioinformatics)
Syllabus:
1. Fundamentals of Bioinformatics (18 Questions))
Bioinformatics: an introduction, introduction about available computational tools and
databases on bioinformatics sites, biological literature information access, storage and
retrieval.
Bioinformatics Resources: pair wise sequence alignment and multiple sequence