EMD Millipore—with the expertise of Calbiochem ® , Chemicon ® , and Upstate ® EMD Millipore is a division of Merck KGaA, Darmstadt, Germany FEATURED ANTIBODY FAMILY: Direct Conjugated Antibodies for Neural Markers Visualizing neural architecture is still critical for much of neuroscience research today and immunostaining remains the standard technique. Our growing line of directly conjugated key markers to neural cell types and subcellular regions adds precision, reliability, and simplicity to the study of focused regions like perisynaptic zones, axons, and glial arborizations. Visit our website for a complete list of neural marker antibodies and direct conjugates and use our convenient online marker charts to plan your next experiment. Visualization of neural structure made easy. Several versatile and convenient technologies have emerged as key tools for the visualization of neural structure. Advances in immunolabeling, lentiviral live cell biosensors, and neuron-specific microfluidic chambers have allowed unparalleled views into the mechanisms of development, outgrowth, protein trafficking, gene regulation, synaptic formation, and degeneration in neural systems. EMD Millipore is a leader in developing innovative neuroscience visualization tools, including our expanding line of direct conjugated antibodies, lentiviral biosensors, and AXIS® microfluidic culture chambers. Stem Cell Cancer Epigenetics & Gene Regulation Signaling Cell Structure Neuroscience ANTIBODIES, ASSAYS, SMALL MOLECULES, INHIBITORS, AND PROTEINS New VOLUME 5 I 2012 products Fluorescent IHC staining of adult mouse brain with Anti-Synaptophysin- Alexa Fluor® 488 conjugate (Catalogue No. MAB5258A4; green) and nuclear counterstain Hoechst 33342 (blue).
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EMD Millipore—with the expertise of Calbiochem®, Chemicon®, and Upstate®
EMD Millipore is a division of Merck KGaA, Darmstadt, Germany
FEATURED ANTIBODY FAMILY:
Direct Conjugated Antibodies for Neural MarkersVisualizing neural architecture is still critical for much of neuroscience research today and immunostaining remains the standard technique. Our growing line of directly conjugated key markers to neural cell types and subcellular regions adds precision, reliability, and simplicity to the study of focused regions like perisynaptic zones, axons, and glial arborizations. Visit our website for a complete list of neural marker antibodies and direct conjugates and use our convenient online marker charts to plan your next experiment.
Visualization of neural structure made easy.Several versatile and convenient technologies have emerged as key tools for the visualization of neural structure. Advances in immunolabeling, lentiviral live cell biosensors, and neuron-specific microfluidic chambers have allowed unparalleled views into the mechanisms of development, outgrowth, protein trafficking, gene regulation, synaptic formation, and degeneration in neural systems. EMD Millipore is a leader in developing innovative neuroscience visualization tools, including our expanding line of direct conjugated antibodies, lentiviral biosensors, and AXIS® microfluidic culture chambers.
Stem Cell CancerEpigenetics & Gene Regulation
Signaling Cell StructureNeuroscience
ANTIBODIES, ASSAYS, SMALL MOLECULES, INHIBITORS, AND PROTEINSNewVOLUME 5 I 2012
products
Fluorescent IHC staining of adult mouse brain with Anti-Synaptophysin-Alexa Fluor® 488 conjugate (Catalogue No. MAB5258A4; green) and nuclear counterstain Hoechst 33342 (blue).
Discriminate somas and dendrites (green) from axons (red) and nuclei (blue) using the AXIS® microfluidic platform. Confocal micrograph of ICC staining of mouse Balb/c P3 mixed cortical neuron culture using the microfluidic AXIS® Confocal slide (AX15005PBC). Somas and dendrites were stained with anti-MAP2 (Catalogue No. MAB3418). Axons were stained using anti-βIII tubulin (Catalogue No. AB15708) and CY3. Nuclei were counterstained with DAPI.
N1E-115 cell line differentiating through an AXIS® device (AX15010) using 20X magnification. (left) Brightfield image of cells showing cell differentiation through the 150 µm wide microgrooves. (middle) Staining of cells with pan-neuronal antibody MAB2300X (green) and DAPI (blue). (right) Overlay of both images to allow visualization of growth within the AXIS® device.
Visualize synapse dynamics with unprecedented clarity by transducing your neurons with LentiBrite™ biosensors. ICC image of live primary rat hippocampal neuron cells transduced with PSD95-GFP lentiviral particles. The PSD95-GFP displays a dotted distribution along the neurites.
EMD Millipore’s newly released Anti-Amyloid β (Aβ) Peptide, clone 6C-3 monoclonal antibody (MOAB-2, clone 63, Catalogue No. MABN254) specifically detects Aβ but not APP. This antibody has been cited in a new study on Aβ deposition in neurodegenerative disease. Katherine Youmans and colleagues describe this unique and highly aggregate-specific antibody in their latest publication entitled: “Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody”*
For greater insight into the forms of Aβ peptide associated with Alzheimer’s disease (AD), the researchers used MOAB2, clone 6C3 to detect multiple Aβ 40 and Aβ 42 conformations, including unaggregated, oligomeric and fibrillar. Not only did MOAB2 consistently detect intraneuronal Aβ without binding to APP, but the antibody also showed greater specificity than did the traditionally used antibody, 6E10. Therefore, MOAB2 is therefore suitable for sensitive and specific detection of accumulating Aβ peptides in AD models.
* Youmans, K. et al. Mol Neurodegener. 2012; 7: 8.
Small Molecules & InhibitorsAPE1 Inhibitor III 262017-10MG
Autophagy Regulators Panel 189488-1EA
β-Catenin/Tcf Inhibitor V, BC21 219334-10MG
BCRP Inhibitor III, YHO-13177 197227-10MG
β-Glucuronidase Inhibitor 347423-10MG
Casein Kinase II Inhibitor VIII 218860-10MG
Choline Kinase-a Inhibitor, CK37 229103-5MG
DUB Inhibitor V, PR-619 662141-25MG
DUB Inhibitor VI, P22077 662142-25MG
EIF2AK3 Activator, CCT020312 324879-5MG
Glutaminase Inhibitor, Compound 968 352010-10MG
GSK-3β Inhibitor XXV 361568-10MG
GSK-3β Inhibitor XXVII 361570-10MG
ICMT Inhibitor II, FTPAT 420351-10MG
ILK Inhibitor, Cpd 22 407331-5MG
Integrin avβ3 Antagonist, P11 407272-5MG
JAK2 Inhibitor IX, WP1193 420151-10MG
KLF5 Inhibitor, CID 5951923 422625-10MG
LDN-211904 428201-5MG
MDM2 Antagonist IV, Nutlin-3a 444152-5MG
Mer RTK Inhibitor, UNC569 445835-10MG
MMP-2 Inhibitor IV 444294-10MG
MT1-MMP Inhibitor, NSC405020 444295-25MG
PDGFRβ/RAF Kinase Inhibitor 521238-10MG
PFKFB3 Inhibitor, 3PO 525330-25MG
PIM-Kinase Inhibitor IX, SGI-1776 526528-10MG
Rac Inhibitor III, EHop-016 553513-10MG
RORa/g Agonist, SR1078 557352-10MG
STAT3 Inhibitor XI, STX-0119 573126-25MG
STAT3 Inhibitor XIV, LLL12 573131-10MG
Tigecycline 610225-50MG
Trk Inhibitor III, GNF-5837 648451-10MG
EMD Millipore’s newly released Anti-LIMD1 monoclonal antibody, clone 3F2/C6 (MABD85) has recently been cited in an important study revealing key molecular mechanisms of hypoxic response. Daniel Foxler and associates published their results in a 2012 Nature Cell Biology paper, entitled: “The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity” *
Having observed that the LIMD1 protein was present in complexes also containing hypoxic response regulators VHL and HIF1a, the authors of the study used the new antibody to localize and quantify coexpression of LIMD1 and HIF1a under hypoxic and normoxic conditions. Complementing these studies with transcriptional knockdown and heterologous expression during hypoxic events revealed a role for LIMD1 in regulating cellular responses. The researchers discovered that LIMD1 simultaneously bound prolyl hydroxylases (PHDs) and VHL, creating the protein complex responsible for degrading HIF1a.
* Foxler, D. et al. Nature Cell Biology 2012; 14:201-208.
EMD Millipore’s newly released Cdc42/Rac1 GTPase Inhibitor, ML141 acts as a highly selective blocker of Cdc42 GTPases. Cdc42 regulates cell structure and polarity in order to effect cell cycle progression and other processes. The discovery of this inhibitor was recently described: “A potent and selective inhibitor of Cdc42 GTPase” *
In this probe report from the United States National Institutes of Health (NIH) Molecular Libraries Program, Surviladze and colleagues have shown that ML141 selectively blocks the activity of Cdc42 GTPase without significantly affecting any other member of the Rho family of GTPases. This selective inhibitor will be useful in unraveling the role of Cdc42 in cell adhesion, cytoskeletal rearrangements, cell motility, and migration.
* Surviladze, Z et al. Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-. 2010 Feb 27.
PUBLICATION HIGHLIGHT ON NEw INHIBITORS:
Description Catalogue No.
Proteins & EnzymesCHIP (c-Myc tagged), active 23-053
microtubule attachment, and melanosome aggregation
in a dose-dependent manner (~10 to 40 µM). Shown to
perturb protein trafficking within the primary cilia and
cause a significant increase in intraflagellar transport
protein 88 (IFT88) levels at the distal tip of primary
cilia. Reported to disrupt primary cilium-dependent Gli
regulation and block Hedgehog signaling.
Calbiochem®: Publish or Perish
Phagophore
LC3
LC3Autophagosome Autolysosome
AutophagyInduction
Lysosome
Low NutrientsExerciseAdiponectinGhrelin
LKB1
CAMKK2
Growth Factors
Ulk1/2
ProteinAggregate
ProteinAggregate
Ulk1/2
FIP200Agt13
mTORmTORC1
AKTAMPK
P
P PP
P P
NH
NH
NHO
O
CI
CI
CI
CN
10
VOLUME 5 I 2012
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