New Functions For Plant PR-5 (Thaumatin- like) Proteins
Jan 14, 2016
New Functions For Plant PR-5 (Thaumatin-like)
Proteins
•Ubiquitous
Plant PR-5 Protein Facts
Osmotin mRNA per 10 ug total RNALa Rosa et al., (1992) Plant Physiol. 100:409
(De Vos et al., 1995)
C
N
N
C
***
**
*
**
(PDB) (Koiwa et al., 1997)
PR-5 Proteins Have Conserved Structure
** * *
* * *
* ** * * *
* *
*
Thaumatin, Osmotin TLPs, OLPs
Polyacrylamide disc electrophoresis of the soluble leaf proteins from
Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’ II. Changes in protein constitution after infection with tobacco mosaic virus
L. C. Van Loon and A. Van Kammen Laboratory of Virology, State Agricultural University, Wageningen, The
Netherlands Accepted 14 August 1969. Available online 16 April 2004.
AbstractElectrophoresis in 5, 7.5, and 10% acrylamide gels revealed that characteristic changes appear in the protein constitution of N. tabacum var. ‘Samsun,’ ‘Samsun NN,’ and N. glutinosa after infection with tobacco mosaic virus (TMV). Apart from significant quantitative changes, one new band was present in Samsun plants 4 weeks after infection. This band was characterized as TMV coat protein by coelectrophoresis and serology. A change in the electrophoretic mobility of the major band was recorded.In Samsun NN plants four new bands (I–IV) were present 1 week after infection. These bands are not related to TMV coat protein and are not new isozymes of peroxidase, polyphenoloxidase, acid phosphatase, glucose-6-phosphate dehydrogenase, or 6-phosphogluconate dehydrogenase. The bands first appear at the onset of necrosis and increase in intensity with time. Five days after infection band I ceases to increase, whereas bands II, III, and IV increase up to day 14. From day 7 onward, the four bands are also present in the noninoculated, young developing leaves, where they further increase with time. The amount of the four new protein components was related to lesion density and was reduced by treatment of the leaves with actinomycin D 2 days after infection.In N. glutinosa one new band was induced and two bands increased markedly after infection, while one had disappeared from the protein pattern. These changes relate to other protein components than those found in infected Samsun NN, but are also apparent in the noninoculated parts of the plants from day 7 after infection.The possible relation of the new protein components to the phenomenon of systemic acquired resistance is discussed.
Virology Volume 40, Issue 2, February 1970, Pages 199-211
Pathogen PerceptionSignalTransduction
RIP
PR-2sPR-1s
PR-5s
LTPsPR-14
Proteinase inhibitors PR-6
ThioninsPR-13
DefensinsPR-12
Chitinases PR-3,-4, -8, -11
Defense genes encoding antimicrobials
PR-5 Proteins Are Plant Defense Effectors
In Fond Memory
Pathogen
PR-7
RNAsePR-10
Other
Transgenic Overexpression Of PR-5 Genes Increases Stress Tolerance
Plant species PR-protein Donor Stressor Tolerance (changea) Agrostis palustris PR-5 Oryza sativa Sclerotinia homeocarpa +Brassica napus PR-5 Hordeum vulgare Plasmodiophora brassicae +Citrus sinensis PR-5 Solanum lycopersicum Phytophthora citrophthora +Daucus carota PR-5 Or. sativa Alternaria ,Aternaria,
Alternaria, Botrytis, Rhizoctonia, Sclerotinia,, Sclerotium
+
Dianthus caryophyllus osmotin Nicotiana tabacum Fusarium +Fragaria x ananassa thaumatin
osmotinThaumatococcus danielliiNi. tabacum
Botrytissalt
++
Gossypium hirsutum osmotin Ni. tabacum drought +(greenhouse)
Hyacinthus orientalis thaumatin Th. danielliii Fusarium culmorum +Nicotiana tabacum osmotin
thaumatinPR-5
Ni. tabacumTh. danielliiOr. sativa
drought, saltPythium, Rhizoctonia,Alternaria
+++
Olea europaea osmotin Ni. tabacum cold +Oryza sativa PR-5
Chitinase + TLP + Serine-threonine kinase
Or. sativa Or. sativa
RhizoctoniaSarocladium ,Xanthomonas
++
Solanum lycopersicum osmotin +chitinasethaumatin
Ni. tabacum,Phaseolus vulgarisTh. daniellii
FusariumPhytophthora
++
(greenhouse)Solanum tuberosum osmotin Ni. tabacum Phy. infestans +Triticum aestivum PR-5
osmotinOr. sativaHo. vulgareNi. tabacum
Fusarium Fusariumsalt
- (field)
+ (geenhouse)
+
0rgan Subcellular pI Loc N-ext C-ext rt ros fl po sd
At1g18250 AtTLP-1 Antifungal f l 8.8 S (1) 20 (20) 4
At1g19320 sd, si 5.7 S (1) 25 (25 S) 0
At1g20030 5.1 I (5) 2 71
At1g73620 f l mic 9 I (4) 41 TM 4
At1g75030 AtTLP-3 Antifungal f l 4.7 S (1) 23 (22 S) 3
At1g75040 PR-5; SAR fl, tri cw /apo, vac 4.7 S (1) 23 (23 S) 0
At1g75050 4.6 S (1) 34 (23 S) 3
At1g75800 4.8 S (1) 22 (22 S) 82
At1g77700 6.4 I (3) 88 56
At2g17860 4.2 S (1) 22 (22 S) 4
At2g24810 10 S (2) 26 (26 S) 0
At2g28790 f l, rt,sd, si cw /apo 7.4 S (1) 26 (21 S) 0
At4g11650 AtOSM34 rt 6.1 S (1) 22 (22 S) 21
At4g24180 4.5 S (1) 27 (27 S) 4
At4g36000 5.4 S (3) 28 (28 S) 4
At4g36010 4.7 S (1) 22 (22 S) 51 GPI
At4g38660 4.4 M (5) 29 (9 T) 100 GPI
At4g38670 f l 8.6 C (5) 20 (78 T) 34
At5g02140 f l 8.4 S (1) 20 (20) 53
At5g24620 4.5 S (1) 26 (26) 169 MA
At5g40020 sd 7.9 S (1) 27 (21) 8
Cluster A acidic 1 to 9
Cluster B neutral 10 to 99
Cluster C basic 100 to 999
>1000
Locus Literature Organ-specific gene expressionProteomics
Protein information
In vitro activity
ORF-based predictions
Arabidopsis thaliana TLPs: characteristics and tissue-specific expression.
Adv Bot Res (2009) 17: 439-489.
Insect Bact
Phyto
phth
ora
infe
sta
ns
Ery
sip
he
chic
ora
cearu
m
Botr
ytis
cin
ere
a
Myzus
pers
icae
Pseudom
onas
syringae
SA
MeJA
AC
C
Osm
otic
Salt
Dro
ught
Cold
Dextr
ose
K low
N low
At1g18250
At1g19320
At1g20030
At1g73620
At1g75030
At1g75040
At1g75050
At1g75800
At1g77700
At2g17860
At2g24810
At2g28790
At4g11650
At4g24180
At4g36000
At4g36010
At4g38660
At4g38670
At5g02140
At5g24620
At5g40020
Nutrients
Senescence
Abiotic stressesLocus Defense-related gene expression
Fungus Hormones
>100 X induction
< 2 X repression
2 to 4.99 X repression
5 to 9.99 X repression
10 to 99 X repression
< 2 X induction
2 to 4.99 X induction
5 to 9.99 X induction
10 to 99 X induction
Induction Of Arabidopsis TLPs By By Stress, Senescence And Nutrients
Adv Bot Res (2009) 17: 439-489.
The PR-5 Protein Gene Family In A Plant Species Is Fairly Large
Species Arabidopsis Rice Poplar Grape
Loci (#) 21 30 51 28
Adv Bot Res (2009) 17: 439-489.
Occurence of PR-5/Thaumatin-like Proteins
•Sequence and structural similarity with plant PR-5 proteins•As families
Yes No
Flowering plants (All)Mosses (All)Algae (All)Amoeba (Some)Fungi (Most)Insects (Some)Bacteria (Some)CyanobacteriaViruses
Mammals (All)Bony fishes (All)Birds (All)
Sp
ecie
s
Adv Bot Res (2009) 17: 439-489.