Neuroscience research brochure

NeuroscienceInspiring technology for creative scientists

Fast, reliable tissue dissociation

Simple, effective myelin removal

Primary neural cell isolation in as little as one hour

Contrast agents specifically optimized for small animal imaging

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Introducing a new milestone in cell analysisThe MACSQuant™ Analyzer paves the way to successful research

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3 Sample preparation

• Time-savingandstandardizeddissociationofneuraltissues

• Removalofmyelindebrisforbetterresultsfromadulttissuesamples

6 Cell separation

• MACS®Technology,thegoldstandardincellseparation

• Pureastrocytes,microglia,neuronsandoligodendrocytes

• Standardizationwithautomatedcellseparation

10 Cell culture

• Serum-freemediumandsupplementforlong-termviability

• Premium,GMP,andresearchgradecytokines

12 Neuroimaging

• Contrastagentsoptimizedforsmallanimalimaging

• MRI,opticalimaging,CTandultrasound

9 Cell analysis

• Titratedhigh-qualityantibodiesandbrilliantfluorochromes

• Easy-to-usebench-topflowcytometers

14 Molecular analysis

• Fastisolationoffunctionalmitochondria

• EfficientmRNAamplificationfromsmallsamples

• GenomicServicesformRNAandmicroRNAexpression

Contents

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Introducing a new milestone in cell analysisThe MACSQuant™ Analyzer paves the way to successful research

Informational brochure

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Tissue dissociation kitsThe secret of success in any experiment lies in the preparation of the starting material. Get going fast with Miltenyi Biotec’s NeuralTissueDissociationKits(NTDK)andthegentleMACS®Dissociators that help streamline and standardize the generation of single-cell suspensions.

• Gentleandefficient:titratedenzymesandoptimizedbuffers

• Conservedepitopesforoptimaldownstreamapplications

• Convenience:everythinginonebox,includingdetailedprotocols

• Reproducibleresultsfromstandardizedcomponents

In order to conserve the epitopes of interest, it is important to choose the correct protease because some epitopes are degraded by trypsin, others by papain. Two kits were therefore developed, NTDK (T) and NDTK (P).

Antigen compatibility of Neural Tissue Dissociation KitsTable 1 shows the recommended kit regarding antigen compatibilityforsubsequentcellseparationoranalysis.EpitopesensitivitiesweretestedwithMACS®Antibodies.

Sample preparationGet a good start

Figure 1: Flow cytometric analysis of cells dissociated with trypsin-based NTDK (T) and papain-based NTDK (P) and then labeled with Anti-GLAST-PE. After trypsin-based dissociation the epitope is conserved and 20% of the cells are positive for GLAST whereas after papain-based dissociation the antibody binds to only 6%. Thus, the NTDK (T) is recommended for use with Anti-GLAST products. For detailed information visit www.macsneuroscience.com/info and download poster 1.

NTDK (T) NTDK (P)

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Antigen Species Cell type

Neural Tissue Dissociation Kits (P)

Prominin-1 Mouse Neural stem and progenitor cells

A2B5 Human, mouse, rat Glial-restricted precursors

O4 Human, mouse, rat Immature oligodendrocytes

CD11b Human, mouse Microglia

CD81¹ Mouse, rat Microglia, macrophages,endothelial cells, glia

CD31¹ Mouse Endothelial cells

CD133 Human Neural progenitor cells

AN2 (mouse NG2)²

Mouse NG2 glia

Neural Tissue Dissociation Kits (T)

Prominin-1² Mouse Neural stem and progenitor cells

O4 Human, mouse, rat Immature oligodendrocytes

PSA-NCAM Human, mouse, rat Neuronal precursors, oligodendrocyte progenitors

CD24 Mouse Neuronal precursors, ependymal cells

A2B5³ Human, mouse, rat Glial-restricted precursors

CD11b Human, mouse Microglia

CD271 (LNGFR)

Human Schwann cells, motor neurons

CD105 Mouse Endothelial cells

Ter-119 Mouse Erythrocytes

GLAST Human, mouse, rat Astrocytes, radial glia

CD133 Human Neural progenitor cells

AN2 (mouse NG2)²

Mouse NG2 glia

1 Slight epitope sensitivity with the use of the papain-based kit; therefore, a further dilution of the enzyme mix is recommended. (1:10;5µLinsteadof50µL)2 Incubation for re-expression of antigen necessary.3 Slight epitope sensivity with the use of the trypsin-based kit; therefore, use of the papain-based kit is recommendedEpitope sensitivities have been tested with antibodies available from Miltenyi Biotec.

Table 1: Antigen compatibility with papain (P) and trypsin (T)-based kits

If the epitope is not sensitive to proteases, we recommend the papain-based kits.

• NeuralTissueDissociationKit(T)*and(P)*

• BrainTumorDissociationKit,human(T)*and(P)*

• NeuralTissueDissociationKit–PostnatalNeurons(P)

• NeurosphereDissociationKit(T)and(P)

• EmbryoidBodyDissociationKit,humanandmouse**

*ThesekitscanbeusedforevenmorereliableresultswiththegentleMACSDissociators. Protocols for manual dissociation using pipettes are also available. **KitcanonlybeusedwiththegentleMACSDissociators.

GLAST

20% 6%

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Automated tissue dissociationTeam the tissue dissociation kits with the gentleMACS Dissociatorsfor:

• Ultimatereproducibility,independentofuser

• Asafeandsterileclosedsystem

• Push-buttontechnologyinsteadofpipetting

• Processingmultiplesamplesinparallel

“… one of our research focuses is related to the role of brain-intrinsic immune cells in malignant brain tumors, especially in the most malignant variant, the glioblastoma …

The best results were obtained by using the gentleMACS® system in combination with the Brain Tumor Dissociation Kit. “

Michel Mittelbronn,Ph.D, Institute of Neurology Goethe-University Frankfurt, Germany

The gentleMACS Dissociators provide different programs for gentle preparation of single-cell suspensions or for homogenization.

Up to two samples can be processed with the gentleMACS Dissociator and up to eight samples at a time with the gentleMACS Octo Dissociator.

Visit www.macsneuroscience.com/videos to watch the preparation of neural tissue with the gentleMACS Dissociator.

Sample preparationGet a good start

Figure 3: Neuronal precursors in culture. The cells show the same morphology in culture after (A) manual dissociation using NTDK (T) and after (B) gentleMACS dissociation using NTDK (T). ßIII-Tubulin (green), PSA-NCAM (red).

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B

“Your digestion kit has not only improved our yields but the preparation has much less debris. I wish I had used it when we initiated these studies.”

Richard Ciavarra,PhD, Eastern Virginia Medical School, USA

Figure 2: The gentleMACS and gentleMACS Octo Dissociators shown with a tissue dissociation kit, C-Tubes, and M-Tubes.

The gentleMACS Dissociators not only combine reliability and reproducibility in an easy-to-use system, the resulting cells do not look any different from cells prepared manually.

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Sample preparationGet a good start

Removal of myelin debrisMyelin Removal Beads II

• Effectivelyremovesmyelindebrisfromsingle-cell suspensions

• Usewithrodentbrainolderthan1weekandhumansamples

• Improvesantibodybindingindownstreamapplications

• Noneedfordeterminationofcell/debrisratioorcellnumbers

“Myelin removal beads allow us to reliably and quickly separate the “trash from the treasure”.

Noel Derecki from the Kipnis lab., Department of Neuroscience, University of Virginia, USA

Figure 4: Staining of microglia from post-natal (P22) mouse brain with CD11b-APC. 1×106 cells from a single-cell suspension of P22 mouse brain were stained with CD11b-APC without (left) and with (right) previous myelin removal using Myelin Removal Beads. The dot plots show that in samples with previous myelin removal, higher percentages of CD11b-positive cells are stained. Dead cells were excluded using propidium iodide. Only the positive cells along with positive debris are displayed in side and forward scatters. For detailed information visit www.macsneuroscience.com/info and download poster 2.

Forward scatter

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Figure 5: Removal of myelin debris from single-cell suspensions with Myelin Removal Beads. Postnatal (P22) mouse brains were dissociated using the Neural Tissue Dissociation Kit (P) and the resulting single-cell suspensions analyzed by flow cytometry either before or after treatment with Myelin Removal Beads. (A) Single-cell suspensions derived from mouse brain consist of large amounts of myelin membrane fragments and only 4% cells. (B) Myelin Removal Beads efficiently remove myelin debris.

before myelin removal

after myelin removal

Forward scatter

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4.48% 16.67% 89.0%

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Isolation of specific cell types from tissue samples or cell cultures,e.g.,ESoriPScells,isaprerequisiteforexactanalysis,efficient screening and optimal cell culture.

ThisisMACS®Technology:thegoldstandardinbench-topcellseparation with more than 14,500 publications to prove it. This renowned technology is also available for neuroscience research:

• Preparationofastrocytesormicrogliain1hourinstead of two weeks by the shake-off method

• Gentlerprocedurethanflowsorting,simplerthanimmuno-panning

• Optimalrecoveryandhighpurity

• Directlyonyourbench,noexperiencerequired

• Sterilesamplehandling

MicroBeads• Small,non-toxic,biodegradable

• Conjugatedtohighlyspecificmonoclonalantibodies

• Compatiblewithflowcytometryanalysis

MACS Columns• Amplifythemagneticfield,onlysmallamounts ofMicroBeadlabelingrequired

• Cell-friendlysteelmatrix

• Depletionandenrichmentofupto2×1010 total cells

Watch the isolation of astrocytes with Anti-GLAST (ACSA-1) MicroBeads on www.neuroscience.com/videos.

Cell separationThe fast track to pure, viable cells

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A B C

Figure 6: The features of MicroBeads and MACS Columns(A) Scanning electron microscopy of a cell isolated with MACS MicroBeads (B) 50 nm MicroBeads are so small they can only be seen on a Transmission Electron Microscope (C). A cross section of a MACS Column showing the steel ball matrix (gray) with the magnetic field in which labeled cells (purple) are retained.

Magnetic separation

MACS®ColumnTechnologyprovides a high-gradient magnetic field.

• Gentletocells

• Thoroughrinsingprocedure

• Highrecovery

Unlabeled cells are collected in the flow-through

Magnetic labeling

Gentle to cells; minimal influence on downstream experiments

Elution of the labeled cell fraction

Optimal results–even for rare cells–by using positive selection

Figure 7: The principle of MACS Technology for depletion or enrichment of cells.

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Table 2: Examples of cell separation reagents available for neuroscience research

“We are studying neuron-glia interactions using primary cultures of highly purified neurons and glial cells. Previously, we isolated cells by immunopanning.

… we switched to magnetic cell isolation kits from Miltenyi Biotec because of three advantages. First, the cell preparation is more economical, second, it takes two instead of six hours, and third, it delivers very pure, healthy cells.”

Frank W. Pfrieger Ph.D. Institute of Cellular and Integrative Neurosciences (INCI), France

Original fraction

Negative fraction

Positive fraction

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11b

Forward scatter

Figure 8: MACS Technology enables isolation of cells even from adult tissue samples. Enrichment of human microglia from a glioblastoma sample achieved a purity of 99%. For detail information visit www.macsneuroscience.com/info and download poster 3.

Cell type Product Species

Neurons

Retinal ganglion cells

Retinal Ganglion Cell Isolation Kit Rat

Neuronal precursors

Anti-PSA-NCAM-MicroBeads Human, mouse, rat

Neurons Neuron Isolation Kit Mouse

Astrocytes

Astrocytes and radial glia

Anti-GLAST (ACSA-1) MicroBead Kit

Human, mouse, rat

Astrocytes Anti-ACSA-2 MicroBeads (coming soon)

Oligodendrocytes

Immature oligodendrocytes

Anti-O4 MicroBeads Human, mouse, rat

Oligodendrocyte precursors

Anti-A2B5 MicroBeads Human, mouse, rat

NG2 glia/ Polydendrocytes

Anti-AN2 MicroBeads Mouse

Schwann cells CD271 (LNGFR) MicroBead Kit Human

Neural progenitors

Neural progenitors CD133 MicroBead Kit Human

Neural progenitors Anti-Prominin-1 MicroBeads Mouse

Neural crest cells CD271 (LNGFR) MicroBead Kit Human

ES/iPS derived Neural crest cells

Neural Crest Stem Cell MicroBeads (coming soon)

Microglia

Microglia CD11b (Microglia) MicroBeads Human, mouse

T cells

T helper cells CD4 (L3T4) MicroBeads Mouse

Regulatory T cells CD4+CD25+ Regulatory T Cell Isolation Kit, mouse

Mouse

Dendritic cells

Dendritic cells CD11c MicroBeads, mouse Mouse

Direct conjugates of monoclonal antibodies and MicroBeads are optimized and already titrated for you

Figure 9: MACS Technology is optimal for effective downstream applications. (A) Human brain biopsies were dissociated using the Brain Tumor Dissociation Kit (P) and the gentleMACS Dissociator. Myelin was removed using Myelin Removal Beads, and cells isolated using CD11b (Microglia) MicroBeads. The microglia show a normal morphology.(B) Astrocytes (7 DIV) isolated from P1 mice using the Anti-GLAST (ACSA-1) MicroBead Kit. Astrocytes were stained using Anti-GLAST (ACSA-1) (green) andAnti-GFAP(red).CellswereculturedinMACS®NeuroMediumandMACS®SupplementB27PLUS,0.5mML-glutamineand1%Pen/Streponpoly-D-lysine-coated coverslips.

A B

9.1% 0.75% 98.7%

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Cell separationThe fast track to pure, viable cells

“In contrast to very low numbers of microglia obtained with conventional cellular depletion methods, we could increase the purity to more than 95% by using CD11b Microglia MicroBeads.

Based on our long-term experience, we can highly recommend the products from Miltenyi Biotec. These products allow a time-efficient and reproducible cell separation, also in a high-throughput manner and with an excellent quality.“

Michel Mittelbronn,Ph.D, Institute of Neurology Goethe-University Frankfurt, Germany

Manual cell separationAll that is necessary for manual separation is a MultiStand with a MACS Separator, columns and our separation reagents.

TheStarterKitsaretheeasiestwaytobegincellseparation:

• Simple:thekitcontainseverythingyouneed–separator,columns and MicroBeads

• Flexible:orderwithantigen-specificMicroBeadsofyourchoice

• Value:saveonthepriceofindividualcomponents

Automated cell separationTheautoMACSPro®Separatorisabenchtopautomatedmagnetic cell sorter for the isolation of virtually any cell type fromanyspecies:

• Convenient:standardizedwalk-awaycellisolation

• Versatile:isolatevirtuallyanycelltype,andalsoremovemyelin debris

• Simple:anintuitivetouchscreeninterfaceandpresetprograms for optimal results

• Flexible:withhigh-speedsortingofmorethan10millioncells per second and volumes from 0.2 mL to 50 mL.

• Time-saving:Multiplesampleswithlesshands-ontime

Figure 10: OctoMACS Separator with MS Columns on MACS MultiStand

Figure 11: autoMACS Pro Separator

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See for yourself how MACS Cell Analysis can be at the heart of your experiments. Watch the video on www.macsneuroscience.com/videos

MACS AntibodiesBrilliantfluorochromesandhighqualityantibodiesforbrighterstaining and even better data, particularly for flow cytometry.Choose from a large panel of antibodies against mouse, rat and human antigens.

For neural markers please visit www.macsantibodies.com to download the complete antibody list

Figure 13: The MACSQuant Analyzer family.MACSQuantAnalyzer: a bench-top cell analyzer for highly sensitive multicolor flow analysis, with uptotenparameters.MACSQuantVYB:aversatileopticallayout,poweredbya561nmlaserforfluorescentproteinslikeGFP,YFPandmCherry.

Cell analysisAnalyze millions of cells in seconds

MACSQuant® Analyzers• Easytouse,bestinclass,flowcytometryforexpertsand

beginners alike

• 3Lasersandupto10opticalparameters

• Absolutecellcounting

• Rarecellanalysis

• 96-wellautomation

• Compactbench-topdesignalsoaddsvaluetoacorefacility

• Live,aroundtheclock,remotesupport

A B C

Figure 12: Cells from P1 (A), P3 (B) and P7 (C) mouse cortex were stained with Anti-AN2-PE and Anti-A2B5-APC. AN2+ cells are in the twoupperquadrants,whileA2B5+ cells are in the upper right and lower rightquadrants.Thepercentageofcellsthatarepositiveforbothmarkersisshownintheupperrightquadrant.ThedotplotsshowasignificantdecreaseofA2B5positivecellswithincreasinganimalage,sothattheoverlap with AN2 disappears.

Revolutionize neural cell analysis with a range of premium qualityantibodies,brilliantfluorochromes,andnovelinstrumentation. Discover the benefits of flow cytometry.

• Asanalternativetoimmunohistochemistryandcytochemistry, a flow cytometer will measure millions of cells in seconds and enables the analysis of cell populations using multiple markers for a more accurate assessment of the whole cell population

• ComplementingWesternBlotting,aflowcytometercananalyzeproteinswithquantitativeanalysisonacellforcellbasis, analyzing up to eight proteins at once rather than one at a time

Flowcytometryenablestheexactquantificationofcellpopulations and analysis of overlapping markers. Dot plots depict cells and smaller particles as dots (events) and illustrate a staining for a marker by a shift on the respective axis.

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1.9% 3.2% 4.3%4.8% 4.9% 0.4%

14.0% 11.6% 4.4%

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Cell cultureThe medium makes the difference

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B

Figure 14: (A) Astrocytes and (B) oligodendrocytes show excellent growth and morphology in MACS Neuro Medium and MACS Supplement B27 PLUS, 0.5 mM L-glutamine and 1% PenStrep on poly-D-lysine-coated coverslips. Astrocytes (4 DIV) were isolated from P1 mice using Anti-ACSA-2 MicroBeads (coming soon), and oligodendrocytes were(5DIV)isolatedfromP3miceusingAnti-O4-MicroBeads.Astrocyteswere stained using Anti-GLAST (ACSA-1) (green) and Anti-GFAP (red).

The MACS Cell Culture product line for neuroscience includes specially formulated cell culture medium, cytokines and growth factors. Get the best for your cells and promote their growth and differentiation in vitro , by working with a great team.

MACS Supplement B27 PLUS and MACS Neuro Medium• Serum-freesupplementforastrocytes,neuronsand

oligodendrocytes

• BasedonB27withoptimizedcomponentsforinvitropropagation of all mouse, rat, or human neural cells

• Serum-freecellculturemedium

• Promotesoptimalgrowthandlong-termsurvivalofallmouse, rat, or human neural cells

Related products:Small moleculesLamininStem cell-specific media

Visit www.macs-stemcells.com for more information

Basic media: Visit www.macscellculture.com

MACS CytokinesA comprehensive range of cytokines is available for the promotion of neural differentiation and cell maintenance.

• SuperiorqualityincludingpremiumandGMP-grade

• Standardizedhighbiologicalactivity

• Convenientpackagingwithsmallorbulkfillings

Visit www.macscytokines.com to download the cytokine product list.

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A

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Figure 15: Whole mouse brain was dissociated using the Neural Tissue Dissociation Kit (P) and the gentleMACS Dissociator. Neural stem cells were isolated with Anti-Prominin-1MicroBeadsandsubsequentlycultivatedin MACS Neuro Medium supplemented with MACS Supplement B27 PLUS, penicillin/streptomycin, 2 mM L-glutamine, 20 ng/mL MACS Cytokines Human EGF and Human FGF-2. Primary neurospheres formed after one week in culture. (A) The Neurosphere Dissociation Kit (P) was used for the dissociation of primary neurospheres. (B) Secondary neurospheres formed in the same medium and cytokines.

Figure 16: Neural/neuronal cells differentiation of human iPS cells to peripheral neurons.Pluripotent human iPS cells were isolated using the TRA-1-60 MicroBead Kit prior to neural induction with dorsomorphin. At day 10 of differentiation, neural crest stem cells were enriched with Neural Crest Stem Cell MicroBeads. Differentiation to peripheral neurons was performd in MACS Neuro Medium with N2 Supplement, NGF, BDNF, dcAMP and ascorbic acid for three weeks. For more detailed information visit www.macsneuroscience.com/info and download poster 4.

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NeuroimagingInsights into neural function

Smallanimalimaging(SAI)techniquesareusedtoinvestigatemodels of human disease, such as Alzheimer’s Disease. State-of-the-art instrumentation coupled with sensitive and specific contrast agents, such as the Viscover™ range of pre-clinical contrast agents, allow researches to obtain high-fidelity anatomical and functional information from animal studies in vivo.

Modality Product group

Applications

MRI GadoSpin™ • Braintumors• CompromisedBBB• Neuroinflammation• Stroke• MS• Angiography• DCEmeasurements• Angiogenesis• Moleculartargetedimaging

FeraSpin™ • Different size-selected nanoparticles• CNSinflammation• Osteomyelitisandasepticvertebral

inflammation• Stroke• MS• Alzheimerdisease• Angiography• rCBV• Microvesseldensityimaging• Celltracking• Moleculartargetedimaging

FeraTrack™ Ready-to-use iron oxide particles for ex vivo intracellular labelling of cells prior to transplantation and tracking

CT ExiTron™ • Softtissuecontrast• Angiography• Angiogenesis

Ultrasound PolySon™ • Perfusionstudies• Moleculartargetedimaging

(see figure 22)

Optical Imaging

NiroWave™ • CNSinflammation• Stroke• MS• Alzheimer'sdisease• Angiography• Vascularleakage• Angiogenesis• Braintumor

Viscover quality• Ready-to-usewithstep-by-stepinstructions

• Excellenttolerability–only100μLinjectionvolume

• Translatabletotheclinic

• Iso-osmolarsterileformulation

• Singledosebolusadministration

SAItechniqueshaveevolvedfromhumanapplicationsintheclinic, including magnetic resonance imaging (MRI), computer tomography (CT), ultrasound (US) and optical imaging (OI). Of these,MRIandOIarefrequentlyusedinneuroscienceinvestigations in small animals although there are Viscover productstosuiteachapplication:

Magnetic resonance imagingGadoSpin™ contrast agents are based on gadolinium chelate and can be used to examine angiogenesis in brain tumors and neuroinflammation where the blood-brain barrier has been compromised.

Figure 17: Intracranial tumor in mouse exhibits contrast enhancement after GadoSpin M has passed the compromised blood-brain barrier. The image series shows tumor progression.

Figure 18: MRI image of mouse after intracerebral injection of GadoSpin F and intravenous injection of Gadospin D and FeraSpin XS. Image shows brain and spinal cord (purple) and the vasculature (red).

Table 3: SAI applications and Viscover imaging agents

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NeuroimagingInsights into neural function

Figure 19: Mouse whole-body T1-weighted MR angiography using FeraSpin XS.

Figure 20: In vivo MRI imaging of mouse cortex: Extracellularly and intracellularly FeraTrack™ labeled PSA-NCAM + cells were grafted into the cortex of a mouse. A strong contrast was detected for the FeraTrack™ labeled cells in the left cortex at 7 Tesla MRI (Prof M. Hoehn, Max Planck Institute for Neurological Research, Cologne).

Optical imagingNiraWave™ optical imaging contrast reagents enable the study of disease-related changes in vascular permeability, for example, the breakdown of the blood-brain barrier. NiraWave can also be conjugated to antibodies in the same way as fluorochromes, to enable targeting.

Figure 21: Optical angiography in mouse injected with NiraWave nano 780. The fine structure of blood vessels is visible.

Ultrasound imagingPolySon™ Ultrasound microbubbles have been used, for example,todetectthedistributionoflesionsandtoquantifythe expression of ICAM-1 in the study of experimental allergic encephalitisinthemouse:

Figure 22: Detection of compromised BBB in EAE mouse model by i.v. injection of anti-ICAM-1 conjugated PolySon H microbubbles using Ultrasound Imaging: Anti-ICAM-1-PolySonH binds specifically on the surface of lesions that express ICAM-1.

Find out more at www.viscover.com

FeraSpin™ contrast agents are based on iron oxide nanoparticles and can be used for various neuroscience applications such as CNS inflammation studies, MRA, rCBV measurements or cell tracking.

The FeraTrack tracking kit simplifies intracellular labeling of cells ex vivo. Cells labeled with FeraTrack can then be tracked in real time by magnetic resonance imaging.

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For more information on our entire molecular range visit www.macsmolecular.com.

ExperiencehowMACSmolecularprovidesexquisitesensitivityin cDNA synthesis, outstanding purity in protein isolation, high recovery in mitochondria isolation, and enables expression profiling of both microRNA and mRNA.

More than just a power house

Are you studying the role of mitochondria in neurodegenerative disease or aging? The Mitochondria Isolation Kit, human and the Mitochondria Isolation Kit, mouse tissue, are based on renowned MACS Technology (seepage6)andenable:

• Pure,intact,viablemitochondriainlessthantwohours

• Higheryieldsofmitochondriacomparedtodifferentialcentrifugation or density gradient centrifugation

• Organelleintegritymaintainedforfullfunctionality

Watch the Mitochondria Isolation Kit in action. Visit www.macsneuroscience.com/videos.

μMACS™ and MultiMACS™ cDNA Synthesis Kits• Time-savingwithonestepinsteadofthree:combinationof

mRNA isolation with in-column cDNA synthesis. With the cDNA magnetically bound to the column, no extra cDNA purification step is needed.

• Highyields

• Ready-to-useenzymereactionmixesinthecompletekit

Molecular analysisLook below the cell surface

Genomic Services - make the most of small samplesIf sample material is minimal or is only available as formalin-fixed paraffin-embedded tissue, Genomic Services can extract that valuable information and provide you with the data you areseeking:

• SuperAmp:analysisof10-10,000cellsbymillion-foldamplification

• microRNAexpressionprofilingbasedon miRXplore™ Microarrays

• Agilentwholegenomeexpressionorarray-CGHanalysis and bioinformatics

• Cellcharacterization,diseasebiology,biomarkeridentification

Send us your sample and we’ll send you documented gene expression data by return. Visit us at www.miltenyibiotec.com/genomicservices.

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Molecular analysisLook below the cell surface

Technical supportWe are well known for the high level of support our team of expertsprovidesourcustomers.Wecanhelpwithanyquestionyouhaveaboutourproductsby:

• On-linelivetechnicalsupport(New)

• Email

• Telephone

• On-linediscussionforums

There are also many articles available for download including

• Scientificposters

• MACS&more–NeuroscienceSpecial

• Datasheets

Simply visit www.miltenyibiotec.com/support We also run customer training at our HQ near Cologne, Germany and on-line customer webinars

For ordering information and the latest news on our products:

macsneuroscience.com [email protected]

The proof is in the publications! Visit www.macsneuroscience.com/references for the extensive list of literature on successful research conducted with our neuroscience products.

Further informationSupport and links

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Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.

Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, gentleMACS, gentleMACS Octo, AutoMACS Pro, μMACS, MultiMACS, MACSQuant, Viscover and the MACS Logo are registered trademarks or trademarks of Miltenyi Biotec GmbH. Copyright © 2011 Miltenyi Biotec GmbH. All rights reserved.

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