Natural oxygenation according to the Jacquier method · the cell. This is where the final steps of the respiratory cycle take place, when the organic molecules generated during digestion

Natural oxygenation according to the Jacquier® method

HOLISTE

A NEW CONCEPT FOR HEALTH

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A significant increase in the number ofallergies, metabolic disorders, new diseases,chronic fatigue syndrome, sleep apnea,stress… and what if the solution is simplybetter cellular oxygenation?Recent studies constantly prove the role ofhypoxia in current pathologies.

It is expected that 1 in 2 people will have somesort of allergy by 2020 / children are the mainvictims /according to the WHO, this disorderwill be ranked 4th in the most frequentlyrecurring chronic diseases in the world.Fabienne Rancé, Paediatric allergist /researcher at Inserm Pôle U563.

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Located in South Burgundy area ofFrance, Holiste Laboratory designs anddevelops its products using its uniqueknow-how, benefiting the localeconomy with its pioneering eco-designand sustainable development policies.

Bol d’air Jacquier® is a uniqueapplication which improvescellular oxygenation in a simple,natural way, with few constraintsand without hyper-oxygenation orrisks involving free radicals.

Technologyinnovation awards

Holiste Laboratory has developed health applications and innovating, highperformance products, enabling people to manage their own health andwell-being. The company has been involved in the last 25 years in globalhealth methods where human being is no longer seen as a multi-part body, but a “whole” in its biological, psychological and energetic entirety…

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•Partners

The inventorA natural oxygenation•

A chemical engineer, student of the NobelPrize winner Victor Grignard, René Jacquierembarked on a research career. He coordinated work in several fields, such asantibiotics and penicillin extractionprocedures. But his main interest was always in thehormones, enzymes, vitamins and biologicalphenomena which determine “the ideal state ofhealth”.

His combined knowledge of biology, chemistryand physics was applied to the extremecomplexity of ecosystems and of life.

It is in this context that he launched the biocatalytic oxygenation concept thatgives birth to the Bol d’air Jacquier®, whose purpose is to stimulate theself-regulation and healing process that already exists within each of us,using cellular oxygenation.

Today, modern science confirms most of his theories, elaborated more than60 years ago.•

Why do we forget that oxygen is essential for life…?

No doubt it’s because the reality is not so obvious… we lackoxygen even while the air that we breathe contains enough ofit to meet all our needs.

In the long term, increasing the quantity of oxygen we breathewould have a detrimental effect on our health, even though ashort sharp inhalation gives us a pleasant and euphoricsensation, without deeper effects.

• This method is born from the inventor’s concern regarding theharmful effects of hypoxia (a lack of oxygen within the cell): apublic health problem that affects us all and intensifies each day,with the weakening of our body due to life conditions, stress,aging etc.

• Today, the Bol d’air® is the result of progress and technologicalinnovation realised during the past 25 years and more.The published conclusions of recent scientific works attest to theimportance of this simple and natural method, as well as itsirrefutable place in the public health issues of today andtomorrow.

• The Bol d’air® is not a medicine and millions of people in France,Europe and all over the world use it every day!

René Jacquier,

Chemist, researcher, visionary inventor

July 5th 1911 - March 21st 2010

Photosynthesis is the process through which plants capture carbondioxide (CO2) from air and release oxygen. A part of this oxygen suppliesanimal breathing. Another part is used to oxygenate organic matter. Theremainder is released into the atmosphere and used again by plants forthe respiration phase. Therefore, the “footprint” of a forest, over several years, is almost zero.This means it consumes the same amount of oxygen as it generates. TheAmazon forest is not the Earth’s lung.It is probably the ocean that plays the role of atmospheric oxygen regulator.

The plankton on the ocean surface forms an enormous biomass, muchlarger than the terrestrial biomass, and the vegetal component ofplankton (the phytoplankton).It generates extremely large quantities of oxygen, also throughphotosynthesis.However, other recent studies prove that oxygen could also come fromMajorite, a mineral capable of storing oxygen in the depths of the Earth’smantle. As it approaches the earth’s surface, Majorite breaks down andreleases its oxygen, which thus becomes available for the oxidationreactions that represent a precondition of life.Christian Ballhaus from the Mineralogy Institute at Bonn University.

Since the beginning of the Palaeozoic era, about 550 billion yearsago, an equilibrium has been created in the life cycle, maintainingthe oxygen rate in the atmosphere at about 21%, thusencouraging the development of animal life.

Today, despite the enormous additional consumption of oxygengenerated by man for industry and transport, this huge reservoir ofoxygen still appears to be stable.

Oxygen is one of the basic elements of Life on Earth

This oxygen keeps us alive

OXYGEN PLANET

It forms 21% of the air we breathe

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The body knows how to store food, but not how to build up oxygen reserves

Just five to ten minutes withoutoxygen and life stops! Our body

can survive several weeks without

food, a few days without water, but

it cannot survive without

breathing. In fact, breathing is thesource of the energy essential tothe functioning of our organs andthe metabolism of our body,which is the chain ofchemical reactions thatenable cell renewal and thepreservation of life. Deepwithin tissue, oxygenparticipates in thetransformation of nutrientsfrom food into energy.

Everything starts with inhalationThe air that surrounds us contains about 21% oxygen. When we inhale, it penetrates the airwaystraveling deep into the alveoli. Our lungs contain 300-450 million such tiny sacs with extremely finewalls and a surface area of 100 m2.

The gas exchanges occur through this wall irrigated by the blood capillaries: the oxygen from the air isdistributed in the blood. At the same time, the blood releases the carbon dioxide it contains.

Haemoglobin assures its transportIn blood, more than 95% of the oxygen bonds to the haemoglobin, a protein consisting mainly of redblood cells, which serves as a carrier. Within the blood stream, haemoglobin releases oxygen which then penetrates the cells. In return, it captures the carbon dioxide, a waste product of cellular respiration, andtransports it to the lungs, where it is eliminated in the exhaled air.

A basic equation is therefore created: Nutrients + oxygen = energy + carbon dioxide + water

OXYGEN, AT THE HEART OF OUR VITAL PROCESSES•

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•We must breathe continuouslyto remain alive

The mitochondrion supplies the energy

This intracellular organelle is considered to be the power plant ofthe cell. This is where the final steps of the respiratory cycle takeplace, when the organic molecules generated during digestionare transformed into energy directly usable by the cell (ATP).In the presence of oxygen, a glucose molecule can generate 36energy units (ATP), whereas only 2 units are generated without it.

The oxygen in the body

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A human being uses the equivalent of 30 kg of ATP/day…

Hypoxia is the lack of oxygen at cellular level

The hypoxia

WHEN WE LACK OXYGEN…

The obstacle created by pollutants

Pollutants, present in the atmosphere of all urban or industrial areas,decrease the quantity of oxygen present in the tissue. Coating the airways,they slow down the gas exchanges. Some of them, such as carbonmonoxide (CO) can even replace the oxygen in the haemoglobin! In allcases, breathing efficiency is diminished.

Besides that, there is the impact on the current way of life, where stressand emotional shock lead to a fall in blood pressure, and consequently inoxygenation.

Carbon monoxide (CO) intoxication is the main cause of toxic mortality

in France. The affinity of haemoglobin with this gas is 230 times

stronger than with oxygen. The haemoglobin, deprived of its oxygen,

can no longer act as a carrier.

The loss of life expectancy due to pollution is now clearly established.

• Hypoxia is not caused by an oxygen deficiency in the air, but rather by its insufficient assimilation within the tissue

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The caprices of oxygenation

Another possible origin of hypoxia: the modification of blood characteristics.In actual fact, even if it manages to capture its oxygen, the haemoglobin can only deliver it to the cells under certainconditions, more specifically conditions related to temperature, acidity (pH) or carbon dioxide content. Therefore, if the carbon dioxide content in the atmosphere exceeds 0.06%, it is sufficient to cause the body to start sufferingfrom hypoxia. This almost always occurs in big cities where the level oscillates between 0.09 and 0.14%.

Metabolism is altered

The oxygen deficiency in the tissue leads to a slowing down of energy production. Consequence: the biochemicalreactions for the assimilation and transformation of nutrients are incomplete. The waste products are notcompletely eliminated and then accumulate, progressively poisoning the body. The brain and the central nervoussystem, the biggest consumers of oxygen, are the first to be affected by the decrease of oxygen quantitiesavailable. Consequently, several alarm signals are triggered: chronic fatigue, nervousness, memory disorders.

The cell suffers a double stress

Both an excess or a lack of oxygen are dangerous for the cell. The first generates free radicals, very reactive molecules that destroy the organic structures. Hypoxia (lack of oxygen) goes even further…It causes a dysfunction of the mitochondrion which can no longer supply energy, thus generating free radicals inlarger quantities. Moreover, it leads to a deterioration of the anti-radical defence system / which means it creates adouble stress for the cell.

The disease becomes established

If this hypoxia situation persists, most of the body’s functions are damaged. The loss of vitality leads to thedevelopment of pathologies such as heart and circulatory disorders, chronic hepatitis, atherosclerosis, or evenmore serious diseases such as cancer and other degenerative pathologies.

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The hypoxia

The cell is in constant need of balanced oxygenation •

The Bol d’air Jacquier®

BREATHE MORE EFICIENTLY

How can we fight against hypoxia and stimulate theassimilation of oxygen by the cell? How can we improveboth its transport in blood and its release into the tissuein order to assure an optimal cellular oxygenation in thebody?

Aware of the fact that a simple increase in the quantityof oxygen breathed is not a solution, René Jacquiersearched for a natural element, easy to access, ready tobecome an oxygen carrier and available on a sufficientlyglobal scale so that the long history of its use would bethe guarantee of its harmlessness.

After observing that since ancient times, sitesassociated with health have been located in the middleof pine and fir tree forests, and based on his experience,he found the answer to his questions in pine resin.

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It is not about inhaling more oxygen, but allowingthe cells to better recover the oxygen circulatingin the blood.

Alpha and beta-pinenes are used in more than 280 medicines and

cosmetic products, as an active ingredient or excipient.

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IMPROVES THE BIOAVAILABILITY OF THE OXYGEN WITHIN THE BODY

An active principle

Pine resin essence is particularly rich in unsaturated molecules (i.e. transformable molecules): the pinenes (belonging to the terpenes family)mainly contain Alpha and Beta pinene (the most widespread molecules in the world). They are used in medicine and in cosmetics for theirwell-known beneficial effects (antiseptic, antibacterial, antifungal, antiparasitic, insecticide).

The Bol d’air®

These terpenes acquire new properties when they are transformed in theBol d’air. They become oxygen carriers. Inside the body, they attach tohaemoglobin and become a biocatalyst.

Biocatalysis

It is a double mechanism: on one hand, haemoglobin binds more readilyto oxygenated pinenes than to simple oxygen; on the other hand, oxygentransported in this way is more easily released into the tissue, in a “newlyborn” form, easily assimilated by the cell which uses it with no excess.In the biocatalytic complex, the oxygen/haemoglobin binding is unstable.Oxygen is easily released to the cells. New oxygen can be captured againand restored. This cycle can last several hours.

A balanced oxygenation

The body is better oxygenated. The cell tends to use the oxygen that itneeds without the oxidative stress related to a lack or excess of oxygen.The mitochondrial function improves and allows a plus of energy and animprovement in the anti-radical defence system.

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The Bol d’air Jacquier®

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Outside the body Transformation Inside the body

A balanced oxygenation / a cycle of several hours

Holiste Technology

The Bol d’air Jacquier®

COMPLEX TECHNOLOGY - BASIC USE

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Holiste is the only manufacturer and distributor of Bol d’air® devices. Trademark, registered patent and design.

• No other essential oil or other product canbe used or added for the proper applicationof the method.

• Product of biological agriculture.

The terpenecomponent Orésine®

proposed by theLaboratoire Holiste

does not containallergens such as the

delta 3 carene.

The special terpene component Bol d’air® : Orésine®

The really active principle of the Bol d’air® method, this concentrate is extractedfrom the hydrodistillate of the moor pine (Pinus Pinaster) resin.The terpene liquid thus obtained is a chemotypical turpentine essential oil of aparticular quality and cannot be confused with an industrial product…It has chemical properties due both to the tree species selected and its place ofgrowth, and to the treatment procedures used. Planted on the French andPortuguese moors, the moor pine is the only resinous plant that can guaranteeallergenic harmlessness. Its resin is collected while still respecting the tree andits durability.As all methods using essential oil, the practice of the Bol d'air Jacquier® is notrecommended for pregnant women or during breast feeding. It is also notrecommended for children under 36 months old.

The Bol d’air® is not an aroma diffuser, or an oxygen generator, or an ionizer

A device: the Bol d'air Jacquier®

The Bol d’air® device is used to transform the volatilemolecules resulting from the special terpene componentBol d’air® in order to create a fine mist that is pleasant tobreathe.It consists of a tank containing the terpene concentrate, an electrical catalysis process and an inhalation corolla. A flow of air generates abubbling which releases themost volatile molecules ofhydrodistillate (mainly alphaand beta-pinenes). The aircontaining these aromaticmolecules passes into adevice where they aretransformed into terpeneperoxides carrying oxygen:this is the peroxidationprocess.

The Bol d’air Jacquier®

Other devices are also available: the Bol d’air®

Aero designed for professionals (medical practices, healthcare or thalassotherapy centres) and the Bol d’air® Tonic range for home use.

Non

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A personalized use

For the user, it is only necessary to sit down in frontof the apparatus within 10 cm of the corolla andsimply breathe.Each person uses the Bol d’air® according to theirown needs and preferences: in the form of a 21 daytreatment (at a medical practice or at home),throughout the entire year, for several sessions a dayor in small daily breathing sessions.The healthcare professionals and consultants at theLaboratoire Holiste support and advise the users fora comfortable and personalized use.

Where can we use the Bol d'air®?

> At home:• In the form of an

intensive treatmentlasting one monthseveral times in the year.

• More regularly aspart of everyday life.

> With a healthcare professional

> In healthcare, wellness, thalassotherapy and thermal centres

• This natural oxygenation method is also adopted as anadditional treatment by millions of healthcareprofessionals, aware of its effect on vitality and its impacton all functions of the body.

Made in France

All devices are provided with an electronicmanagement system of its functions.

They guarantee the totally safe use and complywith current EEC standards.

•Aero: 1 to 8 minute sessionsTonic: 3 to 15 minute sessions

Newtechnology

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Tonic

AéroGlass IndigoGranny Framboise

Scientific references

WHEN RESEARCH PROVES THE POTENTIAL OF THE BOL D'AIR® •Bol d’air® and oxygenation

Béatrice Mercier, Bachelor of science in biology and animal physiology, PhD in oxygenation sciences,gathered together a significant bibliography on cellular oxygenation, the role of terpene essences and theadvantages of the tetravalent form of oxygen generated by Bol d’air Jacquier®.Her thesis, defended at the Burgundy university in 2008 and approved by a prestigious jury, opened newperspectives on this unique tissue oxygenation method.She continues her analysis of the Bol d’air® in collaboration with various research teams in severalcountries in Europe.

Bol d’air® and free radicals

The scientific observations, carried out at the Faculty Gabriel of Dijon, highlighted the role of the Bold’air® in the increase of the anti-radical capacity of organisms, in vivo and ex vivo. It is not a matter ofconsuming a special form of food supplement, but of generating an organic reaction during the Bol d’air®

inhalations.Therefore, the method is both oxygenating and non-oxidizing, while the increase of oxygen, currently used inother methods, carries a risk of generating oxidative stress of the cell.Publications Béatrice Mercier, Josiane Prost and Michel Prost.

Bol d’air® and agingOne of the main causes of aging is the formation of free radicals. This is what Bol d’air® does: it creates an anti-radical protection. It has been proved that the glicated haemoglobin levels (producers of large amounts of freeradicals related to aging) are much lower in the subjects who inhale peroxidated terpenes.Béatrice Mercier / International Journal of Occupational Medecine and Environnemental Health.

•In order to deepen the

understanding of cellular

oxygenation mechanisms and to

study new applications, the

Laboratoire Holiste embarked on a

multidisciplinary research project.

This integrates explorations in the

fields of physics, chemistry and

molecular biology, in collaboration

with researchers, university staff

and professors in medicine. Since

2003, new observations, thesis and

publications have confirmed the

importance of this natural

oxygenation method, without risks

involving free radicals.

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Scientific references

Bol d’air®, nutrition and overweight

Since 2003, Italian researchers, nutritionists, endocrinologists and clinicians, P. DeCristofaro, A. Pietrobelli and N.C. Battistini, proved the role of oxygenation in obesity –international congress in San Diego USA (known as FASEB), “Congress on Obesity” inHelsinki and “World congress on obesity” in Prague. They proved that the use of the Bol d’air® encourages both a decrease of the fat mass and an increase of the lean mass,in a significant way when compared with a control group. Since then, the Bol d’air® hasbeen used in specialist centres in Italy as a supplementary treatment and a natural supportto the treatment of obesity and metabolic pathologies.

Influence of the Bol d’air® on the metabolism ofsportspeopleProfessor De Cristofaro proved that during tests measuring constant effort, the respiratoryquotient (RQ) of a sportsperson can significantly decrease following a 6 minute breathingsession. This proves that the Bol d’air® acts on the metabolism, favouring the use of the fatmass. This is of interest to both sportspeople and overweight people.

Bol d’air® and high performance sportDoctor Andrea Lino, specialist in sports medicine, Professor of human physiology at theRome University “La Sapienza”, has observed the remarkable effects of the Bol d’air® onthe explosive force, muscular elasticity and endurance in competitive swimmers.

Bol d’air® and energyESP consulting, an independent research laboratory, hasevaluated in 2012, the effects of the Bol d’air® breathingsessions on healthy sportsmen (a control group versus aBol d’air® group).The impact of the training is important, for the twogroups, on metabolic and respiratory adaptation but theBol d’air® group present a better improvement on at leastthree points:• After 10 weeks training, the maximal oxygen uptake

level during the exercise increases by 2,5% for thecontrol group and by 9,3% for the Bol d’air® group.

• After these 10 weeks, the VO2 max is reduced by 2,8%for the control group and increases by 5,5% for the Bol d’air® group.

• The fatigue score decreased by 9,5% after training forthe control group and by 36,6% for the Bol d’air group®.This improvement is statistically significant. Thus, the Bold’air® represents a great help in the recovery process.

That’s why several athletes of all levels incorporate theBol d’air® breathing sessions in their training.

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The complete list of studies, publications and scientific references on biocatalyticoxygenation and the Bol d’air® can be found at www.holiste.com or obtained by a simple request to the Laboratoire Holiste

Partners•

A natural method

•For teenagers during exam periods, periods ofstress, for all those who want to live better and agemore healthily: use the Bol d’air® every day, as apart of normal life. A few minutes are enough…

During the change of seasons, cold weather, heatwaves, excessive humidity, low sunshine hours,confinement inside buildings, periods of pollendispersion or pollution peaks, when the body hasless resistance and becomes more vulnerable toviral or bacterial activity, as well as to fatigue.

A minimum treatment lasting three weeks, at themost strategic moments, will bringsignificant benefits.

During the “accidents” of life that can weaken thephysical or psychological defence systems:operations, mourning, severe anxiety, lack of sleep.

Before a sport practise.

To accompany the loss of weight.

To optimize other treatments.

Warning:This is not a medicinal product and can’t be assimilated to a medical treatment.For persons with severe reactions: asthmatic, spasmophilic, allergic, hyperthyroid…they just have to progress more slowly.Some very rare cases of allergic reaction have been reported to the chemical family of alpha and beta pinenes.For cases of terpene-dependent epilepsy, there is a contra indication to the Bol d’air®.Not recommended during pregnancy and while breathfeeding.To be avoid for children under 36 months old.Medical advice required.

WHEN SHOULD WE START A BOL D’AIR® TREATMENT?

It can be adopted as a normal part of life by anybody wishing to preserve or restore their basic health, regardless of age and physical condition

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In order to communicate more than

25 years of experience with this

application, and its technical and

biological operation in the light of

the most recent scientific

advances, the Laboratoire Holiste

organises seminars for the

healthcare professionals.

Bol d’air® /TrainingInter-professional meetings

www.holiste.com

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LYON

ARTAIX - BURGUNDY

PARIS

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