National report - Montenegro Regional Workshop 2: Improving Capacity for Diagnosis of Fish and Molluscan Diseases Banja Luka, Bosnia and Herzegovina, 20-24.

National report - Montenegro

Regional Workshop 2: Improving Capacity for Diagnosis of Fish and Molluscan Diseases

Banja Luka, Bosnia and Herzegovina, 20-24 October 2013, Hotel Palas

Production data 2012

Species/tons Fish: Rainbow trout – 500t Molluscs: Mussels – 250t, E.Oyster - ?

Species/farms Rainbow trout - 26 Sea bass/Sea bream - 2 Mussels -16 European oyster -2

Movement/transfers

We need veterinary certificate for any import of fry and mollusk.

Export for Albania Importation of eggs/fry/live fish No data of amounts (Bosnia and

Herzegovina, Croatia, Italy)

Diseases recorded in the country

Viral diseases

Bacterial diseases

Aeromonas hydrophila, Furunculosis , Saddle back diseases, Yersiniosis...

Parasitic diseases – several ecto and endo parazitic diseases

Fungal diseases - Saprolegniosis

Other health problems - lack of oxygen, strong rain...

Diagnostic capacity in the country

Aquatic animal diseases laboratory within country Diagnostic Veterinary Laboratory

Description (diagnostic, research, education) Diagnostic laboratory (not accreditated methods for

fish and mollusk diseases)

Scope and species interest Rainbow trout, Mussels, European oyster

Level of diagnostic

Level of diagnostic:Level 1: Gross observation, general necropsy

(external, internal)Level II: Bacteriology, Parasitology, Mycology,

Histology, CytologyLevel III: Immunoenzyme tests, Molecular

techniques

Analysis of diagnostic capacity

List of diseases and diagnostic methods used (samples VHS, furunculosis, marteiliosis, bonamiosis)

VHS – clinical observation, detection of gross changes,identification by ELISA, RT-PCR

IHN – clinical observation, detection of gross changes, RT- PCR

Furunculosis – Isolation of bacteria on bacteriological media, identification of genus by selective media and general properties, identification of the species by API or any other kit for biochemical identification

Marteilosis, bonamiosis – Cytology, Histology, PCR

Surveillance of nationally listed diseases

Diagnostic methods Monitoring for diseases of fish and mollusk within Operative

programme ELISA, isolation on bacteriological media Sampling / OIE recommendations 12 fish farms 2x per year, For mollusk 2 zones (Kotor – Risan and Tivat bay) 150 animals per zone (2013)

Results No positive cases in 2012.

Surveillance and monitoring of other pathogens

Targeted pathogens and aquaculture species

IPN clinical observation, detection of gross changes,identification by ELISA – no positive in 2012.

Study of abnormal mortality

In a case of abnormal mortality owner inform and veterinary inspector or team of DVL sample fish and mollusk and send in lab for analyses.

Diagnostic methods – same as for monitoring

Mortality description – mortality reported by fishermen, mortality because of other factors (rain, lack of oxygen)

No mortality report in mollusk in 2013 (problems with sea bream)

Conclusions and perspectives

We coduct monitoring programme for fish diseases last five years

Diagnostic of Aquatic animals diseases should be developed.

No collaboration with EURLNot enough trained in fish diseases diagnostic We have started mollusk diseases diagnostic this year. Good collaboration with EURL in La Tremblade, FranceCondacted training in diagnostic of bonamiosis and

marteiliosis

Future needs

Adopting new methods of diagnosticHelp in establishing method of isolation of virus on

cell culturesLab training Better collaboration among regional labsEstablishing coordination with EURL for fish

diseases in Danmark

HVALA NA PAŽNJI!!!!!!!

THANK YOU FOR YOUR ATTENTION!!!!!