1 1 NanoForum09 Las Vegas, NV A NanoForum09 Las Vegas, NV A pril 29, 2009 M. Reed (Yale) pril 29, 2009 M. Reed (Yale) Nanowire FET Biomolecular Sensors Mark Reed Yale University Nanowire FET Nanowire FET Biomolecular Biomolecular Sensors Sensors Mark Reed Mark Reed Yale University Yale University Departments of Applied Physics and Electrical Engineering Yale Institute for Nanoscience and Quantum Engineering with: Eric Stern, David Routenberg, Erin Steenblock, Alek Vacic, Nitin Rajan, Prof. Tarek Fahmy Thanks to: Jin Chen, James Klemic, Daniel Turner-Evans, Pauline Wyrembak , Cathy Jan Labs of Profs. Ronald Breaker, Andrew Hamilton, Tarek Fahmy Departments of Applied Physics and Electrical Engineering Departments of Applied Physics and Electrical Engineering Yale Institute for Nanoscience and Quantum Engineering Yale Institute for Nanoscience and Quantum Engineering with: Eric Stern, David Routenberg, Erin Steenblock, Alek Vacic, Nitin Rajan, Prof. Tarek Fahmy Thanks to: Jin Chen, James Klemic, Daniel Turner-Evans, Pauline Wyrembak , Cathy Jan Labs of Profs. Ronald Breaker, Andrew Hamilton, Tarek Fahmy
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11NanoForum09 Las Vegas, NV ANanoForum09 Las Vegas, NV April 29, 2009 M. Reed (Yale) pril 29, 2009 M. Reed (Yale)
Nanowire FET Biomolecular
Sensors
Mark ReedYale University
Nanowire FET Nanowire FET Biomolecular Biomolecular
Sensors Sensors
Mark ReedMark ReedYale UniversityYale University
Departments of Applied Physics and Electrical Engineering Yale Institute for Nanoscience and Quantum Engineering
with: Eric Stern, David Routenberg, Erin Steenblock, Alek Vacic, Nitin Rajan, Prof. Tarek Fahmy
Thanks to: Jin Chen, James Klemic, Daniel Turner-Evans, Pauline Wyrembak , Cathy JanLabs of Profs. Ronald Breaker, Andrew Hamilton, Tarek Fahmy
Departments of Applied Physics and Electrical Engineering Departments of Applied Physics and Electrical Engineering Yale Institute for Nanoscience and Quantum EngineeringYale Institute for Nanoscience and Quantum Engineering
with: Eric Stern, David Routenberg, Erin Steenblock, Alek Vacic, Nitin Rajan, Prof. Tarek Fahmy
Thanks to: Jin Chen, James Klemic, Daniel Turner-Evans, Pauline Wyrembak , Cathy JanLabs of Profs. Ronald Breaker, Andrew Hamilton, Tarek Fahmy
22NanoForum09 Las Vegas, NV ANanoForum09 Las Vegas, NV April 29, 2009 M. Reed (Yale) pril 29, 2009 M. Reed (Yale)
What I won’t talk about todayWhat I won’t talk about today
nn initial S/N initial S/N ~ 140 (@10fM)~ 140 (@10fM)
⇒⇒ <100 aM limit<100 aM limit(< 3 fg/ml)(< 3 fg/ml)
((1 aM = 30 1 aM = 30 molecule per mmmolecule per mm33))
-20 0 20 40 60 80
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
Nor
mal
ized
|ISD
(A)|
Time (sec)
1 nM 10 pM 100 fM 10 fM
DC, ambient
1212NanoForum09 Las Vegas, NV ANanoForum09 Las Vegas, NV April 29, 2009 M. Reed (Yale) pril 29, 2009 M. Reed (Yale)
DNA sensing: criss-crossDNA sensing: criss-cross
•• Capture1 is the Capture1 is the complementary strand of Probe1;complementary strand of Probe1;•• Capture2 is the Capture2 is the complementary strand of Probe2complementary strand of Probe2
Surface: Capture2Surface: Capture2
-50 0 50 100 150 200
2.0
2.2
2.8
3.0
3.2
|I SD (A
)|
Time (sec)
Probe 1 Probe 2
-50 0 50 100 150 200500.0n
1.0
3.0
3.5
|I SD (A
)|
Time (sec)
Probe 1 Probe 2
SS DD
S D
Surface: Capture1Surface: Capture1
1313NanoForum09 Las Vegas, NV ANanoForum09 Las Vegas, NV April 29, 2009 M. Reed (Yale) pril 29, 2009 M. Reed (Yale)
OTOT--1 reacts to H1 reacts to H--2K2Kbb--SIIN, not HSIIN, not H--2K2Kbb--SIYSIY
2C reacts to H2C reacts to H--2K2Kbb--SIY, not HSIY, not H--2K2Kbb--SIINSIIN
OT-1
2C
Model system for detecting autoimmune diseases and cancerModel system for detecting autoimmune diseases and cancerStern Stern et alet al, , Nano Lett. Nano Lett. 88, 3310 (2008)., 3310 (2008).
OTOT--11 2C2C
HH-- 2
K2Kbb --
SIY
SIY
HH-- 2
K2Kbb --
SIIN
SIIN
XX
1818NanoForum09 Las Vegas, NV ANanoForum09 Las Vegas, NV April 29, 2009 M. Reed (Yale) pril 29, 2009 M. Reed (Yale)
SummarySummarySummary
nn CMOSCMOS--integrable integrable ““NWsNWs””uu LabelLabel--free sensing to aM resolutionfree sensing to aM resolution
nn RealReal--time cellular immune response time cellular immune response uu Applicable to simple, pointApplicable to simple, point--ofof--care diagnosticscare diagnostics