Nano-macro porous glass bone-scaffold provides new Nano-macro porous glass bone-scaffold provides new insight of cell migration insight of cell migration Himanshu Jain, Lehigh University, DMR 0602975 USA. Co-PIs: R. M. Almeida, Lisbon, Portugal; M. Marei, Alexandria, Egypt; W. Soboyejo, Princeton, USA; A.C. Beye, Dakar, Senegal Figure. Top: Confocal microscope images of MG63 osteosarcoma cells showing their migration into the pores - (a) at the surface, (b) at 100 m and (c) at 500 m under the surface. Bottom left. A high level of actin organization develops with parallel stretched fibers (seen green with nucleus in blue), indicating that the cells attach well to the glass. Bottom right. Scanning electron microscope images show that some cells stretch across the pore that is larger than the cell itself, while anchoring on the edge of the macropores. Such ‘jumping/bridging’ across In future, the missing or dysfunctional bone is likely to be replaced by a scaffold that helps the body regenerate its own natural bone. Nano-macro porous bioactive glasses, developed by the Lehigh Materials World Network (MWN) team, are some of the most promising bone scaffold materials. Experiments conducted by this team demonstrate the biocompatibility of the new material including the ability of the cells to infiltrate interconnected macropores (top figures) and then begin forming bone (bottom left). In addition, it has also found an unexpected pattern of cell ‘jumping/bridging’ across the pores (bottom right), indicating a new aspect of glass- cell interactions that would impact the performance of porous scaffolds. (a) (b) (c)