NAME _______________________________________ Circle One: A1 A2 SCORE _____________ /16 MULTIPLE CHOICE Circle the best answer for questions 1-10 (1 pt each) 1. A piece of double stranded DNA has 30% A, what will be the % of G? a. 30% b. 40% c. 20% d. 70% e. not enough information to answer 2. You have a piece of DNA with the sequence ATGC. From this you know, a. A is at the 5’ end b. A is at the 3’ end c. A could be at either the 5’ end or the 3’ end of DNA d. You have a palindromic sequence e. None of the above 3. Which of the following bases pairs with guanine? a. Adenine b. Guanine c. Cytosine d. Uracil e. Thymine 4. The nitrogenous base is linked to the ribose or deoxyribose sugar via a a. Glycosidic bond b. Amide bond c. Hydrogen bond d. Phosphoanhydride bond e. Phosphodiester bond 5. Genomic DNA is _______ resulting in the production of _______. a. Transcribed mRNA b. Translated, tRNA c. Transcribed, DNA d. Translated, protein e. Replicated, mRNA
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NAME_______________________________________
CircleOne:A1A2
SCORE_____________/16MULTIPLECHOICECirclethebestanswerforquestions1-10(1pteach)1. ApieceofdoublestrandedDNAhas30%A,whatwillbethe%ofG?
a. 30%b. 40%c. 20%d. 70%e. notenoughinformationtoanswer
2. YouhaveapieceofDNAwiththesequenceATGC.Fromthisyouknow,
a. Aisatthe5’endb. Aisatthe3’endc. Acouldbeateitherthe5’endorthe3’endofDNAd. Youhaveapalindromicsequencee. Noneoftheabove
3. Whichofthefollowingbasespairswithguanine?
a. Adenineb. Guaninec. Cytosined. Uracile. Thymine
4. Thenitrogenousbaseislinkedtotheriboseordeoxyribosesugarviaa
a. Glycosidicbondb. Amidebondc. Hydrogenbondd. Phosphoanhydridebonde. Phosphodiesterbond
5. GenomicDNAis_______resultingintheproductionof_______.
a. TranscribedmRNAb. Translated,tRNAc. Transcribed,DNAd. Translated,proteine. Replicated,mRNA
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6. _______istheadaptorthatiskeytoconvertingthetripletcodonsofmRNAintotheproteinpolymers.
a. tRNAb. rRNAc. mRNAd. siRNAe. lcRNA
7. Whichofthefollowingstatementsaboutdouble-strandedDNAisfalse?
a. Thebackbonecontainsalternatingribosesugarandphosphategroups.b. Thetwostrandshaveanantiparallelorientation.c. Thehelixhastwogroovescalledthemajorgrooveandtheminorgroove.d. Thebasesareontheoutsideofthehelix.e. Thestrandsareformedbynucleotideslinkedtogetherviaphosphodiester
bonds.
8. Inthepicturebelow,arrowlabeled1correspondsto______andarrow2correspondsto_________.
a. Replication,transcriptionb. Translation,transcriptionc. Mutation,replicationd. Transcription,replicatione. Transcription,translation
9. Inthepicturebelow,whichlabel(s)ontheleftrefertothetemplatestrand?a. 1b. 2c. mRNAd. both1and2aretemplatestrandse. both1andmRNAarethetemplatestrands
10. WhichofthefollowingstatementsareTRUE?
a. ThemRNAistranslatedfrom5’tothe3’endb. ThemRNAistranslatedfrom3’to5’endc. Duringtranscription,themRNAissynthesizedinthe3’à5’directiond. AandCarebothtruee. BandCarebothtrue
3
SHORTANSWER.Followdirectionswitheachquestion.11. Forthebasepairshownbelow,answerthefollowingquestions.(6pts)
a. Shownaboveis(circleallthatapply).
Adenosine Guanosine Cytidine Uridine Thymidine
b. Drawhydrogenbondsbetweenthebasesasdashedordottedlines.c. CIRCLEthegroupthatisthemajordifferencebetweenuridineandthymine.d. Drawanarrowtotheglycosidiclinkageofthepurine.e. Drawasquarearoundthe5’-carbonofthepyrimidine.
12. ThefollowingpieceofdoublestrandedDNAencodesaveryshortpeptide.Inthespace
below,writethesequenceofmRNAthatwouldbesynthesizedbyRNApolymerasefromthispieceofDNAifthebottomstrandisthetemplate.Thenwritetheproteinsequenceinone-lettercodethatwouldbesynthesizedbytheribosomefromthatmRNAusingthefirstreadingframe(useGeneticCodeTablebelow).(0pts)5’- ATGGCTGGGTGCTTTTAA-3’ 3’ –TACCGACCCACGAAAATT-5’mRNAsequence:AUG GCU GGG UGC UUU UAAProteinsequence:MAGCF
N
NN
N
NH2
O
HO
HH
HH
O
O
H O
H H
H H
O
N
HN
O
O
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