Nadh Flim Lasers

PowerPoint Presentation

In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia (http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1102397)For cell culture experiments, used a Ti:S laser (Spectra-Physics-Millennium/Tsunami, 82 MHz, 100 fs pumped by 5W Millenia) along with oil-immersion lens and a dichromatic mirror with 780 nm excitation light. Laser scanning unit and a GaAsp photon-counting PMT connected to a time-correlated single-photon counting system (TCSPC) were used to collect intensity and lifetime data.Differentiation of apoptosis from necrosis by dynamic changes of reduced nicotinamide adenine dinucleotide fluorescence lifetime in live cells (http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1102781)Time-domain FLIM used oil objective lens on TPM microscope with Ti:S Mira F-900 laser, pumped by Verdi laser. Fluorescence photons detected by photon-counting photomultiplier. Time-resolved detection was done by the single-photon-counting SPC-830 printed circuit board with a bandpass filter 450/40 for NADH.

Fluorescence lifetime imaging of free and protein-bound NADH (http://www.pnas.org/content/89/4/1271.full.pdf)Picosecond dye laser system: mode-locked Antares NdYAG laser, CCD from Photometrics (series 200) with thermoelectrically cooled PM-512 CCDIn vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia (http://www.pnas.org/content/104/49/19494.full.pdf)TPM system with TI:S laser (Spectra Physics: Tsunami) with 40x oil-immersion objective, excitation with 800 and 890 nm, ~15 mW. 490 nm short pass filter to collect NADH fluorescence and GaAsP PMT used to collect intensity and lifetime data for fluorescence. Time resolved fluorescence collected with time-correlated single-photon counting electronics (SPC-730).Autofluorescence Lifetime Imaging of Cultivated Cells Using a UV Picosecond Laser Diode (http://link.springer.com/article/10.1023/B:JOFL.0000039351.09916.cc)

Picosecond pulses of a 375 nm laser diode (LDH375 with driver PDL 800-B, pulse duration: 70 ps, 40 MHz, 200W)Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell level (http://www.sciencedirect.com/science/article/pii/S1011134408002625)Femtosecond Coherent laser (judging by specifications, looks same as Mira 900) and used a modified laser scanning confocal Olympus system with a dichroic mirror and water immersion 60x objective. Fluorescence detected with two microchannel plate photomultiplier tubes after a beam splitter, Glan Thompson polarizers, and filters. Signals were amplified into a single photon counting module (SPC830).A UV-Visible-NIR fluorescence lifetime imaging microscope for laser-based biological sensing with picosecond resolution (http://link.springer.com/article/10.1007/s00340-003-1152-4)

Pulsed nitrogen laser (GL-3300, Photon Technology International, Lawrenceville, NJ) at 337.1 nm, repetition between 1 and 20 Hz and output 1.45 mJ with a ICCD (Picostar HR, La Vision, Goettingen, Germany)Time-resolved microspectrofluorometry and fluorescence lifetime imaging of photosensitizers using picosecond pulsed diode lasers in laser scanning microscopes (http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1101542)

Short pulsed laser diode at 398 nm (LDH 400, PicoQuant GmbH, Berlin, Germany) coupled to a laser scanning microscope (LSM410, Carl Zeiss, Germany) with CCD detector from Princeton Instruments Inc., USA.

Fluorescence lifetime system for microscopy and multiwall plate imaging with a blue picosecond diode laser (https://www.osapublishing.org/ol/fulltext.cfm?uri=ol-27-16-1409&id=69698)Diode laser (PicoQuant Model PDL 800-B) at 40 MHz repetition rate, 1 mW avg. power, ~35 ps at 400 nm. Diode laser was coupled into multimode 62.5 um core diameter optical fiber. Output reflected by 45 degree angled mirror onto multiwell plate. Recorded with 8-bit ICCD (Photonic Science ISIS 3)Four-channel time-gated detection system, Ti:S laser (Spectra Physics) for excitation with repetition rate at 80 fs and 81 MHz, with inverted Nikon confocal microscope with 40x objective. Fluorescence detection with photomultiplier in high-speed lifetime imaging module and TCSPC system was used for fluorescence decay measurements.pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells (http://www.mdpi.com/1422-0067/14/1/1952/htm)Laser-assisted fluorescence microscopy and biomedical screening (http://www.mdpi.com/1422-0067/14/1/1952/htm)

Fluorescence recorded with image intensifying detection unit (IMD 4562, Hamamatsu Photonics, Ichino-Cho, Japan) Picosecond pulses from laser diode or mode locked argon ion laser. Recorded with ICCD (Picostar HR 12 image intensifier coupled to cooled ICCD camera; LaVision, Gottingen, Germany) with gate time of width 200-1000psFluorescence Lifetime Imaging with a Blue Picosecond Diode Laser (https://www.osapublishing.org/view_article.cfm?gotourl=https%3A%2F%2Fwww%2Eosapublishing%2Eorg%2FDirectPDFAccess%2F6837F445-BE5E-D53F-02A8D5827F6A938C_117587%2FBIO-2002-MF2%2Epdf%3Fda%3D1%26id%3D117587%26uri%3DBIO-2002-MF2%26seq%3D0%26mobile%3Dno&org=University%20of%20Maryland%20McKeldin%20Library)

Picosecond blue diode laser (pulsed) coupled with optical fiber to illuminate sample imaged onto optical intensifier with phosphor screen read out by CCD camera.