Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA • FUNKE • CASE Chapter 10, part B Classification of Microorganisms
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Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
PowerPoint® Lecture Slide Presentation prepared by Christine L. Case
Microbiology
B.E Pruitt & Jane J. Stein
AN INTRODUCTIONEIGHTH EDITION
TORTORA • FUNKE • CASE
Chapter 10, part BClassification of Microorganisms
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Numerical Identification
Figure 10.9
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
• Combine known antiserum + unknown bacterium
• Slide agglutination
• ELISA
• Western blot
Serology
Figure 10.10
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Western Blot
Figure 10.12
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Phage Typing
Figure 10.13
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• Differences in electrical conductivity between species
• Fluorescence of some species
• Cells selectively stained with antibody + fluorescent dye
Flow Cytometry Uses
Figure 18.11
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• DNA base composition
• Guanine + cytosine moles% (GC)
• DNA fingerprinting
• Electrophoresis of restriction enzyme digests
• rRNA sequencing
• Polymerase Chain Reaction (PCR)
Genetics
Figure 10.14
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Nucleic Acid Hybridization
Figure 10.15
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Nucleic Acid Hybridization: DNA probe
Figure 10.16
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Nucleic Acid Hybridization: DNA chip
Figure 10.17
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 10.5
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Dichotomous Key
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Cladogram
Figure 10.18.1
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Cladogram
Figure 10.18.2
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