University of Kentucky UKnowledge Physiology Faculty Publications Physiology 1-5-2017 Myogenic Progenitor Cells Control Extracellular Matrix Production by Fibroblasts during Skeletal Muscle Hypertrophy Christopher S. Fry University of Texas Medical Branch Tyler J. Kirby University of Kentucky, [email protected]Kate Kosmac University of Kentucky, [email protected]John J. McCarthy University of Kentucky, [email protected]Charloe A. Peterson University of Kentucky, [email protected]Right click to open a feedback form in a new tab to let us know how this document benefits you. Follow this and additional works at: hps://uknowledge.uky.edu/physiology_facpub Part of the Physiology Commons is Article is brought to you for free and open access by the Physiology at UKnowledge. It has been accepted for inclusion in Physiology Faculty Publications by an authorized administrator of UKnowledge. For more information, please contact [email protected]. Repository Citation Fry, Christopher S.; Kirby, Tyler J.; Kosmac, Kate; McCarthy, John J.; and Peterson, Charloe A., "Myogenic Progenitor Cells Control Extracellular Matrix Production by Fibroblasts during Skeletal Muscle Hypertrophy" (2017). Physiology Faculty Publications. 80. hps://uknowledge.uky.edu/physiology_facpub/80
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University of KentuckyUKnowledge
Physiology Faculty Publications Physiology
1-5-2017
Myogenic Progenitor Cells Control ExtracellularMatrix Production by Fibroblasts during SkeletalMuscle HypertrophyChristopher S. FryUniversity of Texas Medical Branch
Right click to open a feedback form in a new tab to let us know how this document benefits you.
Follow this and additional works at: https://uknowledge.uky.edu/physiology_facpub
Part of the Physiology Commons
This Article is brought to you for free and open access by the Physiology at UKnowledge. It has been accepted for inclusion in Physiology FacultyPublications by an authorized administrator of UKnowledge. For more information, please contact [email protected].
Repository CitationFry, Christopher S.; Kirby, Tyler J.; Kosmac, Kate; McCarthy, John J.; and Peterson, Charlotte A., "Myogenic Progenitor Cells ControlExtracellular Matrix Production by Fibroblasts during Skeletal Muscle Hypertrophy" (2017). Physiology Faculty Publications. 80.https://uknowledge.uky.edu/physiology_facpub/80
in GM- (DMEM + 20% fetal bovine serum). Fibrogenic cells were characterized by their
near-uniform expression of Tcf4 (Figure S6A-D). MPCs were characterized by their
expression of MyoD, with greater than 97% of cells expressing MyoD (Figure S6E-H).
Conditioned media was collected following a 24 hr conditioning period. Exosomes were
isolated from MPC CM with the ExoQuick-TC kit (Systems Biosciences, San Francisco,
23
CA) according to manufacturer instructions. Transfection of primary fibrogenic cells and
NIH 3T3 fibroblasts was accomplished using Lipofectamine 2000 (Life Technology)
according to manufacturer instructions. Primary fibroblasts were harvested for RNA and
protein 24 hr following transfection, and luciferase activity of 3T3 cells was measured 24
hr following transfection.
Statistical analysis
A two-way ANOVA, one-way ANOVA or student’s paired t-test was performed to
determine whether a significant interaction existed between factors for each dependent
variable under consideration. Data that demonstrated a non-normal distribution was
log-transformed. Significance was set at P ≤ 0.05.
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Author Contributions
C.S.F. and T.J.K. performed experiments; C.S.F. and T.J.K. analyzed data; C.S.F. and
T.J.K. interpreted results of experiments; T.J.K. prepared figures; C.S.F. and T.J.K.
drafted manuscript; C.S.F., T.J.K., J.J.M. and C.A.P. edited and revised manuscript;
C.S.F., T.J.K., J.J.M. and C.A.P. approved final version of manuscript.
Acknowledgements
The authors would like to thank Janna Jackson, Grace Walton, Alexander Alimov,
Samantha Michaelis, Angel Ho and Sarah White for their technical assistance.
Research was supported by NIH grant AR065337 and the Jeane B. Kempner
Postdoctoral Scholar Award to C.S.F.; NIH grants AG34453 to C.A.P. and
AR060701and AG049806 to C.A.P. and J.J.M.; and the NIH National Center for
Advancing Translational Sciences, through Grant UL1TR000117. The content is solely
the responsibility of the authors and does not necessarily represent the official views of
the NIH. The authors declare no conflicts of interest.
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Figure Legends
Figure 1. Depletion of satellite cells does not influence fibrogenic cell expansion
or myofibroblast differentiation during the first two weeks of overload. A)
Experimental schematic for conditional depletion of satellite cells using the Pax7-DTA
mouse strain. Following tamoxifen or vehicle treatment and a two week washout,
plantaris muscle was mechanically overloaded using synergist ablation for either one
(SA1) or two (SA2) weeks. Representative images of a SC-Dep muscle cross-section at