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Muhammad Ashfaq Pakistan barcode project: progress and applications
30

Muhammad Ashfaq Pakistan barcode project: progress and applications.

Jan 21, 2016

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Page 1: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Muhammad Ashfaq

Pakistan barcode project:progress and applications

Page 2: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 4: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Project execution

Page 5: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Manual catching Pipettes and sieves

Arial nets Light traps Shaking onto sheets

Activities and Progress

Collecting animal parasites

Page 6: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Specimen preparation and databasing

Page 7: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 8: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 9: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 10: Muhammad Ashfaq Pakistan barcode project: progress and applications.

SpecimensAnimals: 34103Plants: 1289

BarcodesAnimals: 28605Plants: 988

IDRC (62%)

18 months

Page 11: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 12: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Pakistan-only BINs/ species

II. Plants: 363

Number of BINs/ species

Page 13: Muhammad Ashfaq Pakistan barcode project: progress and applications.

II. Genera: 698

Taxonomic identifications

III. Species: 764

Page 14: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Barcode Applications

Page 15: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Reference libraries

Page 16: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Helicoverpa armigera

Helicoverpa zea (GU087831)

Heliothis virescens (GU336572)

Masalia sp.

Heliothis sp.

Heliocheilus confertissima

Spodoptera littoralis

Spodoptera litura

Spodoptera cilium

Spodoptera exigua

Mamestra sp.

Mythimna sp.

Leucania venalba

Mythimna convecta

Agrotis segetum

Agrotis ipsilon

Chrysodeixis acuta

Thysanoplusia orichalcea

Chrysodeixis eriosoma

Ctenoplusia albostriata

Trichoplusia ni

Hypocala deflorata

Pandesma robusta

Dysgonia torrida

Achaea janata

Earias insulana

Earias vittella

Pectinophora gossypiella

53

99

90

90

29

30

89

92

72

41

91

2

21

21

41

18

19

98

33

73

24

23

5

86

49

Heliothinae

Amphipyrinae

Hadeninae

Noctuinae

Plusiinae

Catocalinae

Chloephorinae

Gelechiidae

Noctuid species in/around cotton fields of Punjab

Previously only S. litura was reported from cotton in Punjab

Page 17: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Genetic diversity:

mango mealybug, Drosicha

mangiferae

Page 18: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Bt- 1 (36) [AAM1247] Asia II 1

Bt- 2 (1) [ AAM1246]

Bt- 3 (2) [ACB7718]

Bt- 4 (5) [ AAM1248] Asia II 7

Bt- 5 (2)

Bt- 6 (8) [AAM1243]

Bt- 7 (11) [AAM1245] Asia II 5

Bt- 8 (15) [AAM1244] Asia 1

Bt- 9 (2)

Bt- 10 (2) [AAA4495]

Bt- 11 (13) [AAT8875] MEAM1

Bt- 12 (19)

99

99

99

99

99

97

99

99

59

99

84

63 99

52 58

98

29

40

99

91

0.02

Bemisia tabaci, a complex of cryptic species

Page 19: Muhammad Ashfaq Pakistan barcode project: progress and applications.

E

W

N

S

(A) Asia II 1

(B) MEAM 1

(C) Asia 1

Bemisia tabaci, distribution analysis

Page 20: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Dengue epidemic in Punjab

Page 21: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Culex fuscocephala (5; 0.6) Culex perexiguus (1; -) Culex bitaeniorhynchus (4; 0.15) Culex mimeticus (1; -)

Lutzia fuscana (2; 0.0) Culex tritaeniorhynchus (84; 2.01) Culex quinquefasciatus (1025; 1.05)

Culex theileri (3; 0.0) Armigeres subalbatus (45; 0.31)

Culicidae (5; 0.6) Aedes MA03 (3; 0.9)

Aedes MA02 (9; 1.0) Aedes MA04 (45; 1.4)

Aedes aegypti (48; 0.5) Aedes albopictus (175; 1.3)

Aedes MA01 (6; 0.9) Aedes w-albus (10; 0.9) Ochlerotatus pseudotaeniatus (5; 2.0)

Ochlerotatus sp. (2; 0.0) Anopheles subpictus (39; 1.4)

Anopheles peditaeniatus (8; 0.8) Anopheles pulcherimus (8; 0.3)

Anopheles splendidus (1; -) Anopheles stephensi (29; 0.4)

Anopheles sp. nr. dravidicus (1; -) Anopheles annularis (3; 2.4)

Anopheles culicifacies (5; 0.6) Anopheles culicifacies A (1; -)

Mansonia bonneae (1; -) Chironomus kiiensis

100 49

31

13

10

31

13

96

65

62

73

55

41

28

34

31

91

24

21

22

7

8

14

6

35

2

0

0.02

Cule

x Ae

des

Anop

hele

s

Mosquito species in Punjab

Page 22: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Genus: Anopheles Genus: Culex

Genus: Aedes Aedes aegypti Aedes albopictus

Genus-wise distribution of mosquitoes in Punjab

Page 23: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Outreach

Page 24: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 25: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 26: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Barcoding project at NIBGE

Page 27: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 28: Muhammad Ashfaq Pakistan barcode project: progress and applications.
Page 29: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Barcoding team members

Page 30: Muhammad Ashfaq Pakistan barcode project: progress and applications.

Thank you for your attention