www.icddrb.org Solving public health problems through innovative scientific research Presence of multidrug resistant organisms and their genes conferring antimicrobial resistance in the outdoor poultry environment of urban and periurban areas in Bangladesh with their temporospatial diversity Muhammad Asaduzzaman MBBS MPH MPhil Postdoc Assistant Scientist Food Microbiology Laboratory Laboratory Sciences & Services Division
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www.icddrb.orgSolving public health problems through innovative scientific research
Presence of multidrug resistant organisms andtheir genes conferring antimicrobial resistancein the outdoor poultry environment of urban andperiurban areas in Bangladesh with theirtemporospatial diversity
Muhammad AsaduzzamanMBBS MPH MPhil Postdoc
Assistant Scientist
Food Microbiology Laboratory
Laboratory Sciences & Services Division
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Problem Statement
Airborne resistant organism (resistomes) inBangladesh can confer resistance to antibioticswhich has no comprehensive information and thecapacity to carry and propagate resistance of theseresistomes is poorly studied worldwide.
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How project is addressing the problem
1. Determination of the prevalence of resistant bacteria inthe air samples of outdoor poultry environment ofBangladesh.
2. Characterization of antibiotic resistant organisms usingdifferent phenotypic and genotypic methods
3. Exploring the clonal relationship between AMRorganisms isolated from high and low risk areas
4. Identification of the temporal and spatial distribution ofresistant bacteria in outdoor poultry environment ofBangladesh.
Methods
Collection of air samples from
different areas and directly
inoculated on culture plates
Identification of organisms
Antimicrobial susceptibility
testing
Detection of antibiotic
resistance genes
Genetic fingerprinting of
isolates
Test for antibiotic resistance
plasmid
Analysis of ARGs in air
metagenome
Media used in this study
Gram negative bacteria in
MacConkey agar with Cefotaxime& Meropenem
Gram positive bacteria inMannitol Salt agar with Oxacillin
Gram positive bacteria in Slanetzand Bartley agar with Vancomycin
Figure : Different type of subculture plate for the isolation of Gram positive and Gram negative bacteria.