CASE REPORT Mucometra with Persistent Corpus Luteum in Goats SAMIR EL-SHAFEY, M. FATHALLA, I. HABIB AND S. A L-DAHASH* Summary Ovaries of two goats with persistent corpus luteum were studied by histo- chemical methods. Lutein cells showed moderate alkaline phospha- tase and acid phosphatase activity associated with strong delta 5-3,8- hydroxysteroid dehydrogenase and succinic tetrazolium reductase activ- ity. The results are interpreted as an indication that the corpus luteum was functioning. Resume Mucometrie et corps jaunes persis- tants, chez des chevres Cet article rapporte l'etude histo- chimique de deux corps jaunes persis- tants, chez autant de chevres. Les cel- lules luteiniques demontrerent une activite moderee de la phosphatase alcaline et de la phosphatase acide, ainsi qu'une activite intense de la deshydrogenase delta 5-3,B-hydroxy- steroide et de la reductase tetrazolium succinique. Les auteurs interpreterent leurs resultats comme un indice du fonctionnement de ces corps jaunes. Introduction Mucometra, a condition where mucus of variable consistency and amounts collects in the uterus, has been described in cattle, horses and dogs. It is associated with conditions such as imperforated hymen, segmen- tal aplasia of the vagina, cervix and uterus or secondary to longstanding cystic ovarian disease with cystic endometrial hyperplasia (3, 9, 10, 1 1, 13). A similar condition was also noted in beef heifers withheld from breeding for four to five years (7). An unusual case of mucometra associated with persistent corpus luteum has been des- cribed in cows (14). This report provides histoenzymatic studies on the ovaries of two cases of mucometra in goats associated with persistent corpus leteum. Materials and Methods Genital tracts of two Iraqui breed goats were collected from a local slaughter house near Baghdad. Case A - Grossly, the uterine horns were symetrically distended (each horn was 10 cm in diameter) and there was a well developed corpus luteum (CL) on the left ovary (diameter 1.8 cm). The uterus was filled with about one litre of watery fluid admixed with mucoid debris. Case B - The uterus was similarly distended with watery fluid containing mucoid flakes. The CL located on the right ovary was softer and paler than the CL in case A. The ovaries were recovered and processed routinely to obtain fresh frozen sections using cryostat' set at -20°C. Slides were stained for neutral lipids (oil red 0), for phospholipids (12) (Baker's acid hematin-BAH- method), for alkaline phosphatase (AKPase) (5), acid phosphatase (ACPase) (5), for localization of A5-3,8-hydroxysteroid dehydrogenase (A5-313-OHSD) (4) and for succinic tetrazolium reductase (S-TR) (12). Results Case A - Histologically, the CL was normal and mature. Histochemically, the lutein cells (large and small varieties) gave a FIGURE 1 Corpus luteum of case A showing high content of phospholipid in the luteal cells. X 160. strong positive reaction to oil redO0 and BAH (Figure 1) indicating the pres- ence of high amounts of lipids. The lutein cells gave a moderate AKPase and ACPase activity (Figure 2), a strong activity for S-TR (Figure 3) and consistent, strong A5-3/8-OHSD activ- ity (Figure 4). A0 FIGURE 2. Corpus luteum of case B showing ACPase in luteal cells. X63. Can. vet. J. 21: 165-167 (May 1980) *Department of Anatomy, College of Veterinary Medicine, University of Baghdad, Al-Ameria, Iraq. 'Leitz, West-Germany. 165
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CASE REPORT
Mucometra with Persistent Corpus Luteum in Goats
SAMIR EL-SHAFEY, M. FATHALLA, I. HABIBAND S. A L-DAHASH*
SummaryOvaries of two goats with persistent
corpus luteum were studied by histo-chemical methods. Lutein cellsshowed moderate alkaline phospha-tase and acid phosphatase activityassociated with strong delta 5-3,8-hydroxysteroid dehydrogenase andsuccinic tetrazolium reductase activ-ity. The results are interpreted as anindication that the corpus luteum wasfunctioning.
ResumeMucometrie et corps jaunes persis-tants, chez des chevres
Cet article rapporte l'etude histo-chimique de deux corps jaunes persis-tants, chez autant de chevres. Les cel-lules luteiniques demontrerent uneactivite moderee de la phosphatasealcaline et de la phosphatase acide,ainsi qu'une activite intense de ladeshydrogenase delta 5-3,B-hydroxy-steroide et de la reductase tetrazoliumsuccinique. Les auteurs interpreterentleurs resultats comme un indice dufonctionnement de ces corps jaunes.
IntroductionMucometra, a condition where
mucus of variable consistency andamounts collects in the uterus, hasbeen described in cattle, horses anddogs. It is associated with conditionssuch as imperforated hymen, segmen-tal aplasia of the vagina, cervix anduterus or secondary to longstandingcystic ovarian disease with cysticendometrial hyperplasia (3, 9, 10, 1 1,13). A similar condition was also notedin beef heifers withheld from breedingfor four to five years (7). An unusual
case of mucometra associated withpersistent corpus luteum has been des-cribed in cows (14).
This report provides histoenzymaticstudies on the ovaries of two cases ofmucometra in goats associated withpersistent corpus leteum.
Materials and MethodsGenital tracts of two Iraqui breed
goats were collected from a localslaughter house near Baghdad.
CaseA - Grossly, the uterine hornswere symetrically distended (eachhorn was 10 cm in diameter) and therewas a well developed corpus luteum(CL) on the left ovary (diameter 1.8cm). The uterus was filled with aboutone litre of watery fluid admixed withmucoid debris.
Case B - The uterus was similarlydistended with watery fluid containingmucoid flakes. The CL located on theright ovary was softer and paler thanthe CL in case A.The ovaries were recovered and
processed routinely to obtain freshfrozen sections using cryostat' set at-20°C. Slides were stained for neutrallipids (oil red 0), for phospholipids(12) (Baker's acid hematin-BAH-method), for alkaline phosphatase(AKPase) (5), acid phosphatase(ACPase) (5), for localization ofA5-3,8-hydroxysteroid dehydrogenase(A5-313-OHSD) (4) and for succinictetrazolium reductase (S-TR) (12).
ResultsCase A - Histologically, the CL
was normal and mature.Histochemically, the lutein cells
(large and small varieties) gave a
FIGURE 1 Corpus luteum of case A showinghigh content of phospholipid in the luteal cells.X 160.
strong positive reaction to oil redO0 andBAH (Figure 1) indicating the pres-ence of high amounts of lipids. Thelutein cells gave a moderate AKPaseand ACPase activity (Figure 2), astrong activity for S-TR (Figure 3) andconsistent, strong A5-3/8-OHSD activ-ity (Figure 4).
A0
FIGURE 2. Corpus luteum of case B showingACPase in luteal cells. X63.
Can. vet. J. 21: 165-167 (May 1980)
*Department of Anatomy, College of Veterinary Medicine, University of Baghdad, Al-Ameria, Iraq.'Leitz, West-Germany.
165
.....
FIGURE 3. Succinic tetrazolium reductaseactivity in the corpus luteum of case A. X63.
Case B - Histologically, the find-ings were similar to those in case A.
Histochemically, the lutein cellswere less reactive for lipids, AKPase,S-TR and A5-3,f-OHSD (Figure 5).The larger lutein cells were coloreddeeply with ACPase (Figure 6).
DiscussionA condition of goats termed
"'cloudburst" was described by Short(16). Mated does passed through anapparently normal gestation period,
',
4.Hj
4*N
FIGURE 4. Corpus luteum of case A showingstrong activity for A5-316-OHSD In the luteincells. X 160.
t-o1~~~~~~~~~AI
nas atviy inC fcs . NoetedfeecwithFiur 4.X10
bu ttr no memrans or fetu
...,, i :
exeld.A lugtr aC a presentthcevi wa mdrtel close
FIGURhyrmero53Byroxmucomd etyraoge-
Other reportsinCLnofcasB.Nte thatdiffrncte
with Fiur 4.X10
but at term no membranes or fetuswere present and gallons of fluid wereexpelled. At slaughter, a CL was pres-ent, the cervix was moderately closedand hydrometra or mucometra waspresent.Other reports indicate that in the
cow, mucometra developed only in theabsence of luteal tissue (1) and fewcases were associated with cyclic CL(2).
40
FIGURE 6. Corpus luteum of case B showingACPase activity. X160.
In this study, both cases were asso-ciated with functioning CL. The dif-ferences in histochemical charactersrevealed that the associated CLs werefully functioning which may indicatethat they were persistent.The presence of ACPase was consi-
dered a marker enzyme for lysosomes(6), so the presence of reaction pro-ducts reflected autolytic changes.However, in case A, lutein cells wereACPase-positive and this confirms therecent view (8) that the enzyme maynot only be associated with cellularbreakdown but also with steroido-genesis in lutein cells. Supporting evi-dence was gained from observingstrong staining for the enzyme respon-sible for steroid conversion, A5-3,6-OHSD. It has been shown that A5-3/3-hydroxysteroid dehydrogenase plays abasic role in the conversion of Clg andC21 A5-3/3-hydroxysteroids intoA4-3,8-ketosteroids (15). Therefore,we conclude that hydrometra in Iraqigoats can be associated with function-ing CL.
References1. AL-DAHASH, S. and J.S.E. DAVID. The inci-
dence of ovarian activity, pregnancy andbovine genital abnormalities shown by anabattoir survey. Vet. Rec. 101: 296-299.1977.
2. AL-DAHASH, s. and J.S.E. DAVID. Anatomicalfeatures of cystic ovaries in cattle foundduring an abattoir survey. Vet. Rec. 101:320-324. 1977.
3. ARTHUR, G.H. An analysis of the reproduc-tive function of mares based on post-mortem examination. Vet. Rec. 70: 682-686. 1958.
4. BAILLIE, A.H., M.M. FERGUSON and D.M. HART.
Development in Steroid Histochemistry.New York: Academic Press. 1966.
5. BURSTONE, M.S. Enzyme Histochemistry andits Application in the Study of Neoplasms.New York: Academic Press. 1962.
6. De DUVE, c. and R. WATTIAUX. Functions oflysosomes. A. Rev. Physiol. 28: 435-592.1966.
7. DeLANGE, M. The influence of delayed breed-ing on the fertility of beef heifers. Onderste-poort J. vet. Res. 24: 125-362. 1950.
8. ELFONT, E.A., J.P. ROSZKA and M.J. DIMINO.
Cytochemical studies of acid phosphatasein ovarian follicles: A suggested role forlysosomes in steroidogenesis. BiologyReprod. 17: 787-795. 1977.
9. McENTEE, E. The female genital system. InThe Pathology of Domestic Animals.K.V.F. Jubb and P.C. Kennedy, Eds. 2ndEdition. Vol. 1. pp. 487-585. New York:Academic Press. 1970.
166
10. McENTEE, K. Cited by Roberts S.J. Veteri-nary Obstetrics and Genital Diseases. Pub-lished by the author. Ithaca, New York.1971.
11. NACHLAS, M.M., K. TSOU, E. DeSOUZA, C. CHENG
and A.M. SELIGMAN. Cytochemical demon-stration of succinic dehydrogenase by theuse of a new P-nitrophenyl substitutedditetrazole. J. Histochem. Cytochem. 5:420-436. 1957.
12. PEARSE, A.G.E. Histochemistry. Theoreticaland Applied. 3rd Edition. Vol. II. London:J. and A. Churchill Ltd. 1968.
13. ROBERTS, S.J. Veterinary Obstetrics andGenital Diseases. p. 544. Published by theauthor, Ithaca, New York. 1971.
14. ROBERTS, S.i. and F.H. FOX. An unusual caseof bovine mucometra associated with per-sistent corpus luteum. Cornell Vet. 58: 116.1968.
15. SAMUELS, L.T. and L.M. HELMREICH. The
influence of chorionic gonadotrophin onthe 3,-0 1 dehydrogenase activity of testesand adrenals. Endocrinology 58: 435-442.1956.
16. SHORT, R.V. Comparative Endocrinology ofEarly Gestation in Fetal Homeostasis. R.Wynn, Ed. Vol. II, p. 224. New YorkAcademy of Science. 1967.
VETERINARY DRUGS - NOTICES OF COMPLIANCE
NEW DRUG SUBMISSIONS
Common name * Trade name * Manufacturer * Date * Drug use or classification * Dosage form * Animal species
October 1979CAMBENDAZOLE * Equiben horsewormer paste * Merck Sharp &Dohme Canada Limited * 79-10-05 *For the treatment of internal parasiteinfestations due to: large strongyles:Strongylus vulgaris, Strongylus equi-nus, Strongylus edentatus; smallstrongyles (e.g. Trichonema spp.,Poteriostomum spp., Oesophagodon-tus spp.); pinworm: Oxyuris equi;threadworm: Strongyloides westeri;ascarid: Parascaris equorum; hair-worm: Trichostrongylus axei * Paste,each g contains: 0.45 g cambendazole *Horses.
November 1979FAMPHUR * Warbec injectable solu-tion * Cyanamid Canada Inc. * 79-11-20 * For the control of cattle grubs(Hypoderma spp.) * Injection: I mLcontains: 350 mg Famphur * Cattle.MEBENDAZOLE * Telmin suspensionequine wormer * Pitman-MooreLimited * 79-11-15 * Indicated forinfections of: large roundworms(Parascaris equorum); large strongyles(Strongylus edentatus, S. equinus, S.vulgaris); small strongyles (Cyclico-
cycluss spp., Trichonema spp., Trio-dontophorous spp.) and pinworms(Oxyuris equi) * Suspension: I mLcontains 33.3 mg mebendazole *Horses.PYRANTEL PAMOATE * Pyr-a-pamanthelmintic tablets * Rogar/ STBDivision, BTI Products Inc. * 79-11-27* For the treatment of the followingparasitic infections: roundworm andhookworm * Each tablet contains:pyrantel pamoate equivalent to 35 mgof pyrantel base * Dogs, cats.PYRANTEL TARTRATE * Pro-ban-minth premix * Pfizer Canada Inc. *79-11-15 * For the prevention ofmigration and establishment of largeroundworms (Ascaris suum) infec-tions * Premix containing pyranteltartrate 106 g/ kg * Swine.
December 1979CHLORTETRACYCLINE, SULFAME-THAZINE and PENICILLIN * Chlorachel250 swine concentrate * RachelleLaboratories Inc. * 79-12-24 * As anaid in (1) maintaining growth rate andfeed efficiency in the presence of atro-phic rhinitis, (2) maintaining weightgains and stimulating appetite during
periods of stress, (3) the prevention ofbacterial enteritis, (4) the treatment ofbacterial enteritis * Premix, each kgcontaining: chlortetracycline 88.2 g(calculated as chlortetracycline hydro-chloride), sulfamethazine 88.2 g, peni-cillin (from penicillin G procaine)44.10 g * Swine.COUMAPHOS * Baymix (coumaphos)crumbles * Bayvet Division CutterLaboratories Inc. * 79-12-20 * For useas a top-dressing on daily feed rationfor control of gastrointestinal round-worms (Haemonchus spp., Ostertagiaspp., Cooperia spp., Nematodirusspp., Trichostrongylus spp.) * Crum-bles with coumaphos 0.32% as activedrug * Cattle.FENBENDAZOLE * Panacur granules22% * Hoechst Canada Inc. * 79-12-14* For the control of large strongyles,small strongyles, pinworms and ascar-ids * Granules containing fenbenda-zole 222 mg/g * Horses.PENTAZOCINE LACTATE * Talwin-V *Winthrop Laboratories, Division ofStirling Drug Ltd. * 79-12-11 * Anal-gesic * Injectable, each mL contains 30mg pentazocine base as lactate *Horses, dogs.
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