Movement Ecology Lab Exercise Mammalogy Course Led by …€¦ · Movement Ecology Lab Exercise Mammalogy Course Led by Roland Kays North Carolina State University 2013. Lab Exercises

MovementEcologyLabExerciseMammalogyCourseLedbyRolandKays

NorthCarolinaStateUniversity2013

LabExercises1. Roadcrossingtimeforallanimals[GoogleEarth]2. PossumStart/endactivityfromACC3. MaleRaccoonStart/endactivityfromACC4. FemaleRaccoonStart/endactivityfromACC5. ODBAOvertime[excel&pivot]6. Distancemovedpernight,overtime[excel&pivot]7. Interactionsbetweenindividuals.8. Plotdensites(daytimelocations)

1.RoadCrossings

• Question:Whattimeofdaydoanimalscrosstheroads?• Methods:– Downloadgoogleearthdatain1-weekintervalsstarting8/26/13foreachanimal.

– Stepthroughanimalsnightlytracksslowly– Identifycrossings,note‘localtimestamp’,estimatetimefcrossing

– Recorddatainexcelsheet• Results:– CreateGooglemapwithcolorcodeddotsshowinginterestingpatters(species/timeetc…)

– Makeagraphshowingyourresults

2,3,4,StartandEndofActivity• Question:whattimeofdaydotheanimalsstartandendtheiractivity

period?• Methods:

– DownloadACCViewer,besuretoupdateJava,runprogram.https://www.3dyne.com/movebank-acceleration-viewer/download/

– WithinProgram,Downloaddatafromtagid:– Login– Zoomin/out,notestart/endofactivityforeachnightofactivity(float

cursorovercellandnotedate_timeinbottomleft),recordinexcel.NotetimeisUTC,butexcelissetuptoconverttoEST

– Typeinsunset/sunrisetimesforeachdate(suggestonseparatelaptop)notedaylightsavingstime(+1hr)andgetfromhttp://aa.usno.navy.mil/data/docs/RS_OneYear.php

– Noteanyextensivedaytimeactivity• Results

– GraphStarttimeandsunsetovertime– Graphendtimeandsunriseovertime– Graphactivetimeperiodlengthovertime

5.EnergyExpenditureovertime• Question:howdoestheenergyexpenditure/activitylevelvaryovertime?• Methods

– DownloadACCViewer,besuretoupdateJava,runprogram.https://www.3dyne.com/movebank-acceleration-viewer/download/

– WithinProgram,Downloaddatafromtagid:– Login– Experimental/exportODBA,savefile,openinexcel– Makeday,hour,andday_hrcolumninexcel(cut_pasteexcelbluetext)

– UseexcelCrosstabstocalculateaveragevaluesperday,hour,andday_hr.cut/pastethesevaluesintoanewworksheet.

– Repeatforall3animals• Results

– Graphaveragedailyodbaovertime.– Graphaveragehourlyodbaovertime– Graphavergeday_hrodbaovertime– Bonus,plothourlyaveragesforeachdayasseparatelinesonthesamegraph

6.Distancemovedpernight• Question:Howfardidtheanimalsmovepernight.• Methods

– DownloaddatafromMovebankasexcel– Deleteallrowsmissinglocationdata– Sortbyanimal,then‘localtimestamp’– UseUTMcoordinates&Pythagorean'stheoremtocalculatethedistancemovedineachtimestep.

– Makeday,hour,andday_hrcolumninexcel(bluetextinodbaworksheet)

– UseexcelCrosstabstocalculateaveragevaluesperday,hour,andday_hr.cut/pastethesevaluesintoanewworksheet.

– Repeatforall3animals• Results

– Graphaveragedailymovementrateovertime.– Graphaveragehourlymovementrateovertime– Graphavergeday_hrmovementrateovertime

7.Interactionsbetweenanimals• Howoften,andwhere,doanimalsinteract• Methods

– Downloadfrom:http://web.engr.illinois.edu/~klei2/movemine/– Runthethefile:MiningToolsFx.jar– Login:– SelectNCSUCampusCarnivores,Downloadfromserver– SetGap(min)15,ThreshGap(hr)2– ClickMining/DistanceCalculation,saveimage(png)anddata(csv).Lookatdata

– Clickmining/significance.Keep100roundsofsimulation,try20,50,100,250m.Savetheonesyouthinkaremeaningful.

– Clickmining/plottrajectories.GetKMLfile.Tryeachpairofindividuals.Startwith25mand30min,thentryothervariations.

– OpenKML’sinGoogleEarth,saveinterestingmaps.• Results

– Distanceandsignificancematrices– Screen/shotstellingmainresultsofinteractionsites

8.DenSites• Question:wheredoanimalsspendtheday?• Methods– DownloaddatafromMovebankasexcelfile–Makeanewcolumnforhourusinglocaltime– LoaddataintoGISasshapefile–Makemapshowingresults– Summarizeresultsperanimal(#ofdens)andperdensite(#ofvisit)

• Results–Map(s)showingdens– Graphsshowingsummarystatistics

MovebankDownload

1.Movebank.orgClicktheMap

2.SearchNCSUClickmagnifyingglasstozoonClick+toopenanimalsCheckoptions

3.Clicktheibutton‘studiespage’Hasdates,overviewGobacktomap

4.IbuttonDownloadSelectdatatype,downloadtype,daterange

ACCHowTo

• Red/Blue/Greenshowsx/y/zaxesaccelerometervalues• YellowbarsattopshowwhereGPSdataiscollected• Mousewheeltozoomin/out• Click/Dragtomoveright/left• Zoomintoseegraybarsshowingseparatebursts• Floatcursoroverdatatoseedate/timeinbottomleft,inUTC

GoogleEarthHowToUse+/-tozoomin/outontimescale

Movefront&rearslidertocontrolhowmanydotsareshownatonce

Useforward&reversearrowstomoveforward/backwardintimesteps(steplengthwilldependonzoomlevel)

Theseareourresultsfrom2013

Hector’sHomerange

Heidi’sHomerange

Hilda’sHomerange

DailyODBA

ODBA

0

3750

7500

11250

15000

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34

HectorHeidiHilda

Day

Dailyenergyuseperdayoverthecourseofthestudy.Interestingtoseethedifferentoverallvaluesbetweenthethreeanimals,andalsothefewdaysadjustmentatthebeginningaftertheanesthesia(day1)

AccelerometerData:

Activevs.inactiveperiods

Initialdata–beginsduringanesthesiarecovery

PossumRaccoon

ODBA

0

4500

9000

13500

18000

Hour

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 240

5000

10000

15000

20000

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

DailyActivitypatterns(fromODBA)Possumstartsactivitylaterandcontinuesallnight.Raccoonstartsearlierbut

hasmidnightrestfollowedbyearlyAMpulse(bimodal)

Opossumactiveperiodlengthovertime

Ac/vePeriodNum

berofHou

rsAc/ve

0

125

250

375

500

DayNumber

1 6 11 16 21 26 31 36 41 46 51 56

Time

2:10

3:48

5:27

7:06

8:45

Day

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34

SunriseEndTime

Time

17:25

18:03

18:42

19:21

20:00

Day

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34

StartTimeSunset

Maleraccoonstartandendofactivityvs.sunsetandsunrise

TimeofDay

15:20

16:11

17:02

17:53

18:45

StudyDay

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

StartpmeSunset

TimeofDay

2:51

4:14

5:38

7:01

8:25

StudyDay

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

EndpmeSunrise

Femaleraccoonstartandendofactivityvs.sunriseandsunsetPushesactivitymuchclosertodawnthanthemale

Timeofdaytheycrossedroads Noavoidanceofbusyperiodsbyraccoon,butsomebypossum

MaleRaccoon

Num

berof/mes

0

3

6

9

12

Time(military/me)

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

FemalePossum

Num

berof/mes

0

1

2

3

4

Time(military/me)

0 2 4 6 8 10 12 14 16 18 20 22

TotalDailyMovement

NightHectorgothitbyacar

PossibleMeetingLocationsofHector(raccoon)andHilda(Opossum)

ActualMeetingLocationsofHector(Raccoon)andHilda(Opossum):NONE

PossibleMeetingLocationsforHildaandHeidi

ActualMeetingLocationsforHildaandHeidi:NONE

Heidi(Raccoon)andHector(Raccoon)ActualMeetingLocations