Mosquitoes are disease carrying organisms that can negatively impact a customer brand and their success by causing increases in guest complaints and risk of infection. The Ecolab Mosquito Program can help reduce the risk of mosquito bites and complaints by using a comprehensive science based three step program. The program includes a site assessment to Inspect for adult breeding sites and harborages, recommendations for larvicide treatments to Prevent potential breeding locations, and targeted product applications to adult harborages to Protect the property from future mosquito activity. Mosquito traps may also be incorporated to monitor success of the program as well as reduce additional risk in outdoor seating areas such as bars, dining, or other entertainment venues. 1. 2. Identify and inspect larger, natural bodies of water such as ponds, lakes, reservoirs, etc. for evidence of larvae and pupae. Ask the facility manager for a facility map or use Google Earth to identify potentially hidden water sources. Determine whether the body of water is managed by the property. Determine whether there are nearby sources that could also contribute to the mosquito activity on site. Identify potential land depressions, ditches, etc. that could fill with temporary water after heavy rain fall. Inspect gutters, drains and water tanks for larvae – use a larval scoop (Dipper) if necessary. Look for areas of small pooling water, such as around plant pots, potholes, cisterns, flower gardens, and other water bearing containers, and inspect for any larvae infesting the water. Note: Mosquitoes larvae such as Aedes aegypti and Aedes albopictus can develop in as little as a tablespoon of water. Identify areas with broad-leaved or thick vegetation and shaded areas that may be potential adult harborage locations. Inspect cool and dark interior and exterior locations (such as hallways, bathrooms, laundry facilities) for adult mosquitoes. Look for evidence of mosquito activity (live or dead) along windowsills, hallways, bathrooms and under tables or other seating areas and building eaves. Ask the customer about locations where people have complained of being bitten. Consider if some locations are high risk to guests, such as outdoor dining areas, pools, and bars. Prevent future mosquito activity by removing or treating potential breeding and adult resting sites. Make recommendations, as appropriate, to fill in ditches/holes/land depressions where water might pool. Discuss with the customer physical means that can be used to reduce mosquito breeding sites (e.g. emptying buckets, removing plastic containers and plant pot bases, and clearing gutters or drains). Discuss with the customer physical means that can be used to reduce adult harborage sites (e.g. trimming gardens, installing insect screens on windows and doors, and installing fans in rooms or areas where people spend long periods of time outdoors). If treatments to adult breeding sites are required, select the most appropriate larvicide based on the type of water source that needs to be treated (see product guide). Protect the property by applying treatment to the harborage sites and other high risk areas or by installing monitors and other mechanical means of control. Apply residual treatments to mosquito harborage sites and resting sites both inside and outside the building including cool dark hallways, under eaves, around window frames, etc. (see product guide). Apply residual treatments to garden areas, shrubs, and other landscape conducive to mosquito harborages areas. Note: Pay attention to broad-leaved plants and shaded areas when treating gardens. Recommend adding fans for additional air movement or traps to minimize mosquito pressure around outdoor dining or other entertainment areas. Discuss training materials such as the window/door stickers and fact sheets with staff to help minimize risk of mosquito bites and complaints in high risk areas. Recommend fogging for quick knockdown of large populations of adults (for large outdoor events only). Mosquito Program Protocol Quick Reference Guide Ecolab Confidential Information – For Internal Use Only Adult Breeding Sites (Larvae & Pupae) INSPECT, PREVENT & PROTECT INSPECT PREVENT PROTECT Adult Harborage Sites Use the Site Assessment Tool to inspect the site for the presence of larvae, pupae or adult mosquitoes