Monteiro-Riviere ,NA. Indirect Immunohistochemistry and immunoelectron microscopy distribution of eight epidermal-dermal junction epitopes in the pig and in isolated perfused skin treated with bis (2-chloroethyl) sulfide. Toxicologic Pathology 23:313-325, 1995.
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Monteiro-Riviere ,NA. Indirect Immunohistochemistry and ...€¦ · Monteiro-Riviere NA: Integument. In Eurell J, Frappier B, eds. Dellman’s Textbook of Veterinary Histology, Blackwell
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Monteiro-Riviere ,NA. Indirect Immunohistochemistry and immunoelectron microscopy distribution of eight epidermal-dermal junction epitopes in the pig and in isolated perfused skin treated with bis (2-chloroethyl) sulfide. Toxicologic Pathology 23:313-325, 1995.
• Species differences for different mechanisms are independent ∴one species is not the best model for all endpoints in humans!
Species Differences
• Anatomical factors such as thickness, adnexial structures, etc.
• Biochemical differences in lipid composition, enzymes, etc.
• Different receptors for immunological or pharmacological agents
• Physiological differences including blood flow, etc.
Body Site Differences• The rate of penetration and absorption differs
across various body sites• Scrotum > Forehead > Axilla > = Scalp > Back =
Abdomen > Palm and Plantar surface• Seen in humans and animals making route to
route extrapolations “interesting.”• The major reasons are due to:
– Differences in anatomy:skin thickness– Differences in physiology: blood flow and distribution
of blood vessels– Stratum corneocyte cell size ?
Pig Skin is Similar to Human Skin
I like pigs. Dogs look up to us. Cats look down on us. Pigs treat us as equals.
• Similar surface characteristics• Body masses and skin to body surface area ratio• Sparse hair coat• Thick epidermis• Hair follicle density• Epidermal turnover kinetics• Lipid composition• Biophysical properties of lipid
Don’t use skin from an abattoir if hide is scalded!
Differences Between Pig and Human Skin
• Pig skin has an additional interfollicular muscle
• Young pigs have hair follicles that occur in a triad
• Pigs have only apocrine sweat glands over most of the body
• Immunological and drug metabolism differences not well characterized
Monteiro-Riviere, NA. Comparative Anatomy, Physiology, and Biochemistry of Mammalian Skin . In Hobson DW (ed) Dermal and Ocular Toxicology: Fundamentals and Methods, Boca Raton, FL, CRC Press, Chpt. 1, 3-71, 1991.
Porcine Skin
Stromberg MW, Huang YC, Monteiro-Riviere NA. Interfollicular smooth muscle in the skin of the domesticated pig. The Anatomical Record 201:455-462, 1981.
Monteiro-Riviere et al. Interspecies and interegional analysis of the comparative histological thickness and laser Doppler blood flow measurements at five cutaneous sites in nine species. Journal of Investigative Dermatology 95:582-586, 1990.
Blood Flow Measurements of Nine Species at Five Cutaneous Sites
Monteiro-Riviere et al. Interspecies and interegional analysis of the comparative histological thickness and laser Doppler blood flow measurements at five cutaneous sites in nine species. Journal of Investigative Dermatology 95:582-586, 1990.
Hair Follicle Density
Species Area of Skin Number of Hair Follicles/cm2
Human Abdomen 11 + 1Pig Back 11 + 1Rat Back 289 + 21Mouse Back 658 + 38Hairless Mouse Back 75 + 6
Bronaugh et al. Methods for in vitro percutaneous absorption studies II. Animal models for human skin. Toxicology and Applied Pharmacology 62, 481-488, 1982.