14-3-3 Monoclonal ANTIBODY Catalog Number:66061-1-Ig For Research Use Only www.ptglab.com Basic Information Catalog Number: 66061-1-Ig Size: 107 μg/150 μl Source: Mouse Isotype: IgG1 Purif ication Method: Protein G purif ication Immunogen Catalog Number: AG6055 GenBank Accession Number: BC056867 GeneID (NCBI): 10971 Full Name: tyrosine 3-monooxygenase/tryptophan 5- monooxygenase activation protein, theta polypeptide Calculated MW: 28 kDa Observed MW: 31 kDa,28 kDa Recommended Dilutions: WB 1:2000-1:20000 IHC 1:50-1:500 IF 1:20-1:200 Applications Tested Applications: ELISA, FC, IF, IHC, WB Species Specif icity: human, rat,mouse,pig Positive Controls: WB : HEK-293 cells; IHC : human lung cancer tissue; human lung cancer tissue, rat brain tissue IF : HepG2 cells; Background Information 14-3-3 proteins interact with a wide spectrum of proteins and possess diverse f unctions. Mammals express seven distinct 14-3-3 isof orms (gamma, epsilon, beta, zeta, sigma, theta, tau) that f orm multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. 66061-1-Ig recognizes 14-3-3 tau (YWHAQ), eta (YWHAH), and zeta (YWHAZ), but not epsilon (YWHAE). Notable Publications Author Pubmed ID Journal Application Storage Storage: Store at -20ºC. Stable f or one year af ter shipment. Storage Buf f er: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary f or -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll f ree in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data f or this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
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14-3-3Monoclonal ANTIBODYCatalog Number:66061-1-Ig
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:66061-1-IgSize:107 μg/150 μlSource:MouseIsotype:IgG1Purif ication Method:Protein G purif icationImmunogen Catalog Number:AG6055
GenBank Accession Number:BC056867GeneID (NCBI):10971Full Name:tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptideCalculated MW:28 kDaObserved MW:31 kDa,28 kDa
Recommended Dilutions:WB 1:2000-1:20000 IHC 1:50-1:500 IF 1:20-1:200
Applications Tested Applications:ELISA, FC, IF, IHC, WBSpecies Specif icity:human, rat,mouse,pig
Positive Controls:WB : HEK-293 cells;IHC : human lung cancer tissue; human lung cancertissue, rat brain tissueIF : HepG2 cells;
Background Information 14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon,beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have beenimplicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. 66061-1-Ig recognizes 14-3-3 tau (YWHAQ),eta (YWHAH), and zeta (YWHAZ), but not epsilon (YWHAE).
Notable Publications Author Pubmed ID Journal Application
Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (tollf ree in USA), or 1(312) 455-8498 (outsideUSA)
E: [email protected] W: ptglab.com
For technical support and original validation data for thisproduct please contact:
This product is exclusively available underProteintech Group brand and is not available topurchase from any other manufacturer.
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 66061-1-Ig (14-3-3antibody) at dilution of 1:10000 incubated atroom temperature for 1.5 hours
Immunohistochemistry of paraff in-embeddedhuman lung cancer tissue slide using 66061-1-Ig(14-3-3 Antibody) at dilution of 1:500 (under 10xlens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraff in-embeddedhuman lung cancer tissue slide using 66061-1-Ig(14-3-3 Antibody) at dilution of 1:500 (under 40xlens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HepG2 cellsusing 66061-1-Ig(14-3-3 antibody) at dilution of1:50 and Alexa Fluor 488-congugated Aff iniPureGoat Anti-Mouse IgG(H+L)
1X10̂ 6 HeLa cells were stained with 0.2ug 14-3-3antibody (66061-1-Ig, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3%BSA (30 min). Alexa Fluor 488-congugatedAff iniPure Goat Anti-Mouse IgG(H+L) with dilution1:1500.
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