This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
CignalTM Lenti ReportersMonitor Signaling Pathway Activity in Any CellCignalTM Lenti Reporters
Cignal Lenti Reporters
The Cignal Lenti Reporters are ready-to-transduce lentiviral particles for assessing cell signaling activities in virtually any mammalian cell type. This system utilizes a unique combination of robust transcription factor reporter technology and superb lentiviral delivery power. Applications Study Signaling Pathway Activities in Impossible to Transfect Cell Types Immune Cells Stem Cells Neuronal Cells Differentiated Cells Primary Cells Other Difficult-to-Transfect Cells
Transient Transduction Studies to Study the Biological Impact of: Drug candidates or chemical compounds Gene knockdown (siRNA, shRNA) or gene overexpression events Peptides, proteins, or ligands
Generate Pathway Sensor Cell Lines Valuable cell-based assay platform for drug or gene function studies Why Cignal Lenti Reporters? Ready-to-transduce lentiviral particles
Reporter technology exhibiting exceptional sensitivity, specificity, and signal-to-noise ratio
Available with luciferase or GFP as reporter gene
How Cignal Lenti Reporters Work
tel: 301.682.9200 fax: 301.682.7300
Cignal LentiReporter
live lentivirusparticles
TransientTransduction Studies
Transduction w/ Cignal Lenti
AssayReadout
Treatment
Plate Cells
AntibioticSelection
Establish PathwaySensor Cell Lines
Clonal CellLine Isolation
Transductionw/ Cignal Lenti
Plate Cells
High Efficiency Lentiviral Delivery Made These Studies Possible.
s Cignal Finder Lenti Cancer 10-Pathway Reporter ArrayCignal Finder Lenti Immune Response 10-Pathway Reporter ArrayCignal Finder Lenti Development 10-Pathway Reporter ArrayCignal Finder Lenti Toxicity 10-Pathway Reporter Array
Cignal Lenti Positive Control (GFP)Cignal Lenti Negative Control (GFP)Cignal Lenti Positive Control (luciferase)Cignal Lenti Negative Control (luciferase)Cignal Lenti Renilla Control (luciferase)