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・DNA Step Ladders
・OneSTEP Ladders
・Ladders for 10minute separation
・Wide Range Ladders
・Markers & OneSTEP Markers
・DGGE Markers
・PCR Marker Makers
・Easy SeparatorTM
・Negative Gel Stain MS Kit
・SuperSepTM Ace
・SuperSepTM DNA
・RNA Size Standard Markers
・RNA Step Ladder
・Loading Buffers
RNA Si St d
・Prestain markers
・ Western marker
・ Biotinylated marker
・ Markers for CBB dyeing
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・PCR Marker Makers
・Easy SeparatorTM
・Loading Buffers
Molecular-WeightMarkers
DNA Ladder
10 bp DNA StepLadder (10-100 bp)
25 bp DNA StepLadder (25-300 bp)
25 bp DNA StepLadder (25-500 bp)
50 bp DNA StepLadder (50-800 bp)
DNA Step Ladder(10-300 bp)
100 bp DNA Step Ladder(0.1-1.5 kbp)
OneSTEP Ladder 50(0.05-2 kbp)
OneSTEP Ladder 100(0.1-2 kbp)
DNA Step Ladder (1~6)for low-molecular-range analysis.
10 bp DNA Step Ladder (10-100 bp) 25 bp DNA Step Ladder (25-300 bp) 25 bp DNA Step Ladder (25-500 bp) 50 bp DNA Step Ladder (50-800 bp) DNA Step Ladder (10-300 bp) 100 bp DNA Step Ladder (0.1-1.5 kbp)
OneSTEP Ladder 50 (0.05-2.0 kbp)
OneSTEP Ladder 100 (0.1-2 kbp)
OneSTEP Ladder 500 (0.5-5 kbp)
OneSTEP Ladder 1 kb (1-10 kbp)
OneSTEP Ladder Supercoiled PlasmidGene Ladder 100 (0.1-2 kbp)DNA Step Ladder (10-200 bp), Fast Separation TypeDNA Step Ladder (50-1,500 bp), Fast Separation TypeDNA Step Ladder (100-5,000 bp), Fast Separation TypeDNA Step Ladder (500-10,000 bp), Fast Separation TypeGene Ladder Fast 1(4 bands:0.1-2 kbp)Gene Ladder Fast 2 (6 bands:0.1-10 kbp)
DNA molecular-weight markers that set size standards for linear double-strand DNA. Each One-Step Marker comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel.
Wide Range Ladder (19~22)for wide-range and simultaneous analysis of both DNA size and amount.
DNA molec lar eight markers that set si e standards for linear do ble strand DNA E
DNA markers for DGGE (denaturing gradient gel electrophoresis). A CG clamp is added at the end of one side of each fragment to enable separation / inspection by DGGE.
for preparing DNA size markers. Consisting of a mixture of template DNA, primer, and reaction buffer, the reagent initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive control while the PCR is under way.
for preparing DNA size markers Consisting of a mixt
PCR Marker Maker (39~42)
aturing gradient gel electrophoresis) A CG clamp is aaturing grad
Contains protein G with the ability to combine with the Fc region of immunoglobulin. Reacts with both primary and secondary antibodies in a western blot.
Prestained Marker (1, 2) :
Contains biotinylated protein. Capable of both chromogenetic and photogenetic detection by reacting with avidin HRP or avidin AP.Biotinylated Marker (4) :
Prote
in S
ize M
arker
Western Marker (3) :
With dye combined in its recombinant protein content.
-20℃ -20℃ -20℃
Marker for CBB Dyeing (5~8)Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process.
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WIDE-VIEW TM Prestained Protein Size MarkerWIDE-VIEW TM Prestained Protein Size Marker 2WIDE-VIEW TM Western Size MarkerWIDE-VIEW TM Biotinylated Size MarkerMolecular Weight Marker, Low Range Molecular Weight Marker, Middle RangeMolecular Weight Marker, High RangeMolecular Weight Marker, Wide Range
Requires only a small amount (about 250-350ml) of running buffer for electrophoresis.
Allows the gel plate to be removed without removing the running buffer after electrophoresis has been completed.
Features
12
3
Detectable limit of protein: 1 ng
Sensitive and Simple procedure with 2-step staining in 5~10 minutes
Optimized the staining intensity by either de-staining the excess stain or re-staining following de-stain of the gel.
Applicable to proteome analysis by mass spectrometry and sequencing
Featureas
12
3
4
Easy SeparatorTM (Wako Catalog No.058-07681 (1 set ) )
Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting and removal of the gel plate.
Negative Gel Stain MS KitProtein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable, clear and transparent bands against the background of milky white gel stained by negative gel stain containing our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of protein such as sequencing and mass analysis of peptide.
①Remove the cover after electrophoresis has been completed.
①Insert a gel. ②Turn the knobs toward you.
③Setting is completed.
②Turn the knobs away from you.
③Remove the gel without removing the running buffer.
Electr
ophoresis
-rela
ted P
roducts
Negative Gel Stain MS KitKit Contents:1) Staining S olution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)
293-57701 20 tests
Wako Cat. No. Product name Package Size
SDS-PAGE analysis
MALDI-TOF/MS analysis
Figure 1. SDS-PAGE analysis of proteins using Negative Gel Stain MS Kit
a : rat phosphorylase (97k);b : bovine serum albumin (68k);c : hen egg white ovalbumin (45k);d : bovine carbonic anhydrase (31k).Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng
SDS-PAGE gel
Mass Spectrometry
Staining Sol. A
Staining Sol. B
Shake 5 ~ 10 min.Wash 5 ~ 10 sec. x 3
Shake 10 ~ 60 sec.Wash 1 min. x 3
Check staining intensityof the gel against a sheet of black paper
Procedure
Figure 2.
MALDI-TOF/MS of rat phosphorylase
The band was excised and treated with trypsin. Following the in-gel digestion and preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)
17well13wellSuperSepTM AceSuperSep™ Ace is a polyacrylamide gel of the highest quality, designed to resolve the problems of existing products (SuperSep™ and SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to existing products, but because its pH is neutralized, the gel’s chemical storage stability is improved. Physical storage stability is also improved by using vacuum packaging. And because it does not contain SDS, it can be used in SDS-PAGE and Native-PAGE by changing the running buffer.
① SuperSepTM: 10%
②SuperSepTM Ace: 10%
Plate size
Gel size
Number of wells
Well volume
Total amount of protein* (/well)
13 wells
30 μL
3.3~ 6.5 μg
17 wells
25 μL
1.3~ 3.9 μg
100 × 100 × 3 (mm)90 × 85 × 1 (mm)
Product specifications
*Indicates total amount of protein that can be clearly separated.
Good separation and uniform bands
Can be used with a multi-channel pipette.
Reasonably priced and capable of long-term storage (for 6 months)
Box sticker allows easy identification of type of gel.
Comes in 13-well and 17-well types.
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0 1.5 3 4.5 6 7.5 9 10.5 12 (hours)
200
(kDa)
150
10075
50
37
25
20
Improved
separation
near the center!Improved
separation
near the center!
17well13wellSuperSepTM AceSuperSep™ Ace amide gel of the highest qualityis a polyacryla
20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200 bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly separated with TBE buffer.
Features
(bp) (bp) (bp) (bp)
1009080706050403020
200150
25
(bp) (bp)
100
50
30
20
EtBr staining EtBr staining
100
200 200150
100
75
50
25
150
100
75
50
25
90
80706050
40
30
20
(bp)
100
50
3020
200150
25
(bp)
1% Agarose SuperSepTM DNA(15%, 17 well)
SuperSepTM DNA(15%, 17 well)
2.5% Agarose
Example of electrophoresis
Improved
separation
near the center!
1
3
2
The acrylamide concentration, degree of cross-linkage, and number of wells are optimized for the electrophoresis of low-molecular-weight DNA.
Cleaner separation image than that obtained with agarose gel.
Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is therefore effective for the separation of samples with a DNA size from 20-200 bp.
Electrophoresis-related Products
Convenient
sticker for
gel
identification!
EtBr Solution 315-90051 10 mLWako Cat. No.Product name Package Size
(Store at 2~10℃)
Staining Reagent
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Store at or below ー20℃
【How to read the chart】 【Display codes】
10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp
DNA Ladders
10000 b 1000 b 100 b 10 b
10000 b 1000 b 100 b 10 b
Protein Size Markers 10 kDa 100 kDa 1000 kDa
10 kDa 100 kDa 1000 kDa
●1 , p2
●1 , p1
●2 , p2●3 , p2
●6 , p2●7 , p2●8 , p2
●1 , p7●2 , p7
●5 , p7
●3 , p7
●1 , p8●2 , p8
●3 , p8●4 , p8
●5 , p8●6 , p8
●7 , p8●8 , p8
10 b
RNA Ladders
●10 , p2●9 , p2
●11 , p2●12 , p2
●17 , p3●18 , p3
●13 , p3●14 , p3
●15 , p3●16 , p3
●19 , p4●20 , p4
●21 , p4●22 , p4
●26 , p4●27 , p4
●23 , p4●24 , p4●25 , p4
●28 , p4●29 , p4●30 , p4
●31 , p5●32 , p5●33 , p5
●40 , p6●41 , p6●42 , p6
●39 , p6
●4 , p7
【How to read the chart】 【Display codes】Selection Guide
Number Reference page
Store at 2~10℃NO DISPLAY Store at or below ー80℃
Store at or below ー70℃
-80℃
-70℃-20℃
●4 , p2●5 , p2
10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp
08X05開01
co.jpco.jp
09Y3IBF
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