Molecular Techniques in Microbiolo gy These include 9 techniques (1) Standard polymerase chain reaction (PCR): @ Kary Mullis invented the PCR in 1983 (USA) Given Nobel Prize in Chemistry in 1993. @ He was able to amplify DNA by repeated cycles driven by DNA polymerase
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Molecular Techniques in Microbiology These include 9 techniques (1) Standard polymerase chain reaction (PCR): @ Kary Mullis invented the PCR in 1983 (USA)Kary.
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Molecular Techniques in Microbiology
These include 9 techniques
(1) Standard polymerase chain reaction (PCR):
@ Kary Mullis invented the PCR in 1983 (USA) Given Nobel Prize in Chemistry in 1993.
@ He was able to amplify DNA by repeated cycles driven by DNA polymerase enzyme.
@ PCR technique used an automated process for DNA amplification & separation of the DNA strands.
1. DNA extraction: From cell genome DNA by selective amplification of a specific region of DNA. This is done by hybridization تهجين)) probe and DNA clones (نسيالت) techniques to create copies of DNA fragments.
2. DNA sequencing: To determine unknown PCR-amplified sequences by the insertion of a DNA sequence into a genetic material of another organism.
3. DNA-based phylogeny: To determine the evolutionary relationships among organisms.
Extracted DNA
4. Identification of uncultural or slow-growing organisms: Such as mycobacteria, anaerobic bacteria, or viruses.
5. Early diagnosis of infectious diseases:
Such as leukemia and lymphomas; detecting malignant cells at a high sensitivity.
PCR
6. Quantification viral load in a patient (HIV)
7. Diagnosis of hereditary diseases
8. Identification of genetic fingerprints. This is used in forensic sciences and paternity testing