dified Histone Map Modified Histone Map Featured Modified Histone Antibodies www.rockland-inc.com [email protected] Histone H3 [Dimethyl Lys4] Epi-Plus TM is a jointly owned trademark of Novus Biologicals, LLC and Rockland Immunochemicals, Inc. Modified Histone Map © Novus Biologicals, LLC 2011. All Rights Reserved. H4 H4 H2B H2B H3 H3 H2A H2A H2A H2B H3 H2B H4 H4 H2B H2A R T K Q A G G A P Q A T A P T G G V H R R P G V A L R E I R R Y Q S T E L L I R L P F Q R L V R E I A Q D F D L R F Q S S/A A A L Q E A C/S E A Y L V G L F E D N L C A I H A V I M P D I Q L A R R I R G E R A A T S T K R K K A A R K S K K Y T K K K T T K R T K P Ac P P P Ac Me Me Ac Me Ac Me P Ac Me P P Ac Me Ac Me P P Ac P Ac Me 2 3 4 6 10 11 14 17 18 23 26 27 28 36 37 41 45 56 64 79 80 107 115 118 122 Histone H3 G G G G G L G G G A R H R V L R D D N I Q T P A I R R L A R R G G V R I G L I E E T R G V L V F L E N V I R D A V T Y T H E A R T V T A M D V V A L Q G R T L Y G F G S R K K K K K K S Y K K K Y K R P Ac Ac Me Ac Ac P P Me Ac Ac Me P Ac Me 1 3 5 8 12 16 20 31 47 51 59 77 79 88 91 92 Histone H4 L P Ac Me G Cit R 8 Me Cit K Ac 9 Cit Ac A/S 31 Ac Me 87 V/I 89 M/G 90 96 Cit I K 44 G Legend Me P Ac Acetylation Methylation Phosphorylation A - Alanine C - Cysteine D - Aspartic acid E - Glutamic acid F- Phenylalanine G - Glycine H - Histidine I - Isoleucine K - Lysine L - Leucine M - Methionine N - Asparagine P - Proline Q - Glutamine R - Arginine S - Serine T - Threonine V - Valine W - Tryptophan Y - Tyrosine Modifications: Residues: Cit Citrullination Me1 Me2 Me3 Monomethylation Dimethylation Trimethylation Me2 Me1 Me2 Me1 Me3 Me2 Me1 Me3 P Me2 Me1 Me3 Me2 Me1 Me2 Me1 Me2 Me1 Me2 Me1 Me3 Me2 Me1 Ac Me Isoform Residue Changes: Histone H3.2 C96 S96 Histone H3.3 A31 S31 S87 A87 V89 I89 M90 G90 C96 S96 Ac Histone H3 [Trimethyl Lys4] Histone H3 [Dimethyl Lys36] Histone H3 [Phospho Thr6] Histone H3 [Dimethyl Lys18] Histone H3 [Monomethyl Lys36] Histone H3 [Trimethyl Lys4] 600-401-I58 600-401-I59 600-401-I59 600-401-I63 600-401-I79 600-401-I87 600-401-I86