Power Supply 110 - 230 V; 50 / 60 Hz; 240 VA Regulations CE, UL, CSA Temperature Storage: 0° - 40° C Range Operation: 15° - 35° C Humidity 10 – 85%, not condensating Dimensions 472 x 494 x 374 mm (W x D x H), incl. injectors Weight 52 – 54 kg (depending on configuration) Measurement Luminescence Technologies Top-reading Fluorescence Bottom-reading Fluorescence (option) Fluorescence Polarisation (option) Absorbance (option) AlphaScreen™ (option) Detection Unit Low-noise photomultiplier tube in single photon counting mode Excitation Sources Halogen lamp, 75 W, 340 to 700 nm; Laser, 680 nm (option) Excitation Filters 355 nm, 485 nm; 405 nm, 450 nm, 490 nm, 620 nm (with Absorbance option) Emission Filters 460 nm, 535 nm; 400 nm, 515 nm, 480 nm, 530 nm (with BRET option); 568 nm (with AlphaScreen™ option) Performance Luminescence: detection limit <20 amol ATP Fluorescence: detection limit <20 fmol FITC (top reading) Absorbance: linear range 0 – 2 OD Fluorescence Polarisation: c.v. 4.4 % with 1 nM Fluorescein AlphaScreen™: detection limit <12.5 ng standard beads Dynamic Range >6 orders of magnitude Crosstalk Low crosstalk through crosstalk reduction design: 5 x 10 -6 Injection Unit Up to 4 injectors (variable volume: 10 – 100 µl), JET Injection technology Injection into 384 well plates Plate Formats All 96 and 384 well microplates with outer dimen- sions: 86.0 x 128.2 (W x L); tolerance in plate height: 13.9 – 15.1 mm With plate height adjustment option: All 6 well to 1536 well microplates with outer dimensions: 86.0 x 128.2 mm (W x L); tolerance in plate height: 8.0 – 22.2 mm Petri dishes, Terasaki Plates, filters with respective adapters Plate Height Optional: automatic detection and adjust- Adjustment ment to varying plate heights (8.0 to 22.2 mm) Temperature Control Optional: +5°C above RT to 37 °C Includes cooling unit for PMT Operation Modes Integral measurement 0.1 – 600 sec Kinetics measurement (total time up to 24 h) Repeated measurement (total time up to 24 h) Plate repeats (up to 999) Delay (up to 600 sec) Scanning (up to 100 individual measurement points) Dispensing with 4 independently controlled vari- able volume injectors Shaking (3 modes, variable amplitude and speed) BRET and BRET 2 assays Dual-Luciferase® Reporter Gene assays Readit™ parameter file AlphaScreen™ settings FRET settings Ratiometric assays Stacker Stacker20 for 20 microplates (option) Stacker100 for 100 microplates (option) Robotic Integration Optional Interface Serial RS232, 9 pin PC Operating System Win 98, Win 2000, Win NT, Win XP PC Requirements Pentium processor, 500 MHz (or better), CD ROM drive, display 1024x768 (or better), serial port, parallel port Software Windows® application BERTHOLD TECHNOLOGIES MikroWin 2000 Order Information Order Number Mithras LB 940 incl. MikroWin 2000 Lite 940-38099-10 Mithras LB 940 with temperature control incl. MikroWin 2000 Lite 940-38099-15 Injector 1 pre-position L, 10 – 100 µl 940-37772-11 Injector 2 measurement position L, 10 – 100 µl 940-37772-12 Injector 3 measurement position L, 10 – 100 µl 940-37772-13 Injector 4 measurement position F, 10 – 100 µl 940-37772-14 Waste pump (for injectors 1 to 3) 940-38883 Plate Height adjustment 940-39364 Robot integration module 940-39354 BERTHOLD TECHNOLOGIES GmbH & Co. KG Calmbacher Str. 22 • D-75323 Bad Wildbad, Germany Phone +49 7081 177-0 • Fax +49 7081 177-100 [email protected] • www.BertholdTech.com Mithras LB 940 Specifications and ordering informations LB 940 · 042003 · Id.Nr. 81425PR2 Rev02 DLR, DLReady logo and Readit are trademarks of Promega Corporation. Dual-Luciferase and Stop & Glo are registered trademarks of Promega Corporation. AlphaScreen and DeepBlueC are trademarks of PerkinElmer Inc. Windows is a registered trademark of Microsoft. To run certain applications on this product may require licenses from others. PMT cooling unit 940-39337 Bottom-reading Fluorescence 940-39348 BRET package 940-39350 AlphaScreen™ package 940-39359 Absorbance option 940-39360 Fluorescence Polarisation option 940-39351 Stacker20 940-40056 Stacker100 940-40057 MikroWin 2000 Advanced I 37854-003 MikroWin 2000 Advanced II 37854-006
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Power Supply 110 - 230 V; 50 / 60 Hz; 240 VA
Regulations CE, UL, CSA
Temperature Storage: 0° - 40° C
Range Operation: 15° - 35° C
Humidity 10 – 85%, not condensating
Dimensions 472 x 494 x 374 mm (W x D x H),
incl. injectors
Weight 52 – 54 kg (depending on configuration)
Measurement Luminescence
Technologies Top-reading Fluorescence
Bottom-reading Fluorescence (option)
Fluorescence Polarisation (option)
Absorbance (option)
AlphaScreen™ (option)
Detection Unit Low-noise photomultiplier tube in single photoncounting mode
Excitation Sources Halogen lamp, 75 W, 340 to 700 nm;
AlphaScreen™: detection limit <12.5 ng standard beads
Dynamic Range >6 orders of magnitude
Crosstalk Low crosstalk through crosstalk reduction design:5 x 10-6
Injection Unit Up to 4 injectors (variable volume: 10 – 100 µl),
JET Injection technology
Injection into 384 well plates
Plate Formats All 96 and 384 well microplates with outer dimen-sions: 86.0 x 128.2 (W x L); tolerance in plateheight: 13.9 – 15.1 mm
With plate height adjustment option:
All 6 well to 1536 well microplates with outerdimensions: 86.0 x 128.2 mm (W x L); tolerance inplate height: 8.0 – 22.2 mm
Petri dishes, Terasaki Plates, filters with respectiveadapters
Plate Height Optional: automatic detection and adjust-
Adjustment ment to varying plate heights (8.0 to 22.2 mm)
Temperature Control Optional: +5°C above RT to 37 °C
Includes cooling unit for PMT
Operation Modes Integral measurement 0.1 – 600 sec
Kinetics measurement (total time up to 24 h)
Repeated measurement (total time up to 24 h)
Plate repeats (up to 999)
Delay (up to 600 sec)
Scanning (up to 100 individual measurementpoints)
Dispensing with 4 independently controlled vari-able volume injectors
Shaking (3 modes, variable amplitude and speed)
BRET and BRET2 assays
Dual-Luciferase® Reporter Gene assays
Readit™ parameter file
AlphaScreen™ settings
FRET settings
Ratiometric assays
Stacker Stacker20 for 20 microplates (option)
Stacker100 for 100 microplates (option) Robotic Integration Optional Interface Serial RS232, 9 pin PC Operating System Win 98, Win 2000, Win NT, Win XP PC Requirements Pentium processor, 500 MHz (or better), CD ROM
drive, display 1024x768 (or better), serial port,parallel port
Mithras LB 940 Specifications and ordering informations
LB 9
40 ·
042
003
· I
d.N
r. 8
1425
PR2
Rev
02
DLR, DLReady logo and Readit are trademarks of Promega Corporation. Dual-Luciferase and Stop & Glo areregistered trademarks of Promega Corporation. AlphaScreen and DeepBlueC are trademarks of PerkinElmerInc. Windows is a registered trademark of Microsoft. To run certain applications on this product may requirelicenses from others.
Dieser Report wurde automatisch mit Hilfe der Adobe Acrobat Distiller Erweiterung "Distiller Secrets v1.0.5" der IMPRESSED GmbH erstellt. Sie koennen diese Startup-Datei für die Distiller Versionen 4.0.5 und 5.0.x kostenlos unter http://www.impressed.de herunterladen. ALLGEMEIN ---------------------------------------- Dateioptionen: Kompatibilität: PDF 1.3 Für schnelle Web-Anzeige optimieren: Nein Piktogramme einbetten: Nein Seiten automatisch drehen: Nein Seiten von: 1 Seiten bis: Alle Seiten Bund: Links Auflösung: [ 2400 2400 ] dpi Papierformat: [ 595.276 793.701 ] Punkt KOMPRIMIERUNG ---------------------------------------- Farbbilder: Downsampling: Ja Berechnungsmethode: Durchschnittliche Neuberechnung Downsample-Auflösung: 150 dpi Downsampling für Bilder über: 225 dpi Komprimieren: Ja Automatische Bestimmung der Komprimierungsart: Ja JPEG-Qualität: Hoch Bitanzahl pro Pixel: Wie Original Bit Graustufenbilder: Downsampling: Ja Berechnungsmethode: Durchschnittliche Neuberechnung Downsample-Auflösung: 150 dpi Downsampling für Bilder über: 225 dpi Komprimieren: Ja Automatische Bestimmung der Komprimierungsart: Ja JPEG-Qualität: Hoch Bitanzahl pro Pixel: Wie Original Bit Schwarzweiß-Bilder: Downsampling: Ja Berechnungsmethode: Durchschnittliche Neuberechnung Downsample-Auflösung: 800 dpi Downsampling für Bilder über: 1200 dpi Komprimieren: Ja Komprimierungsart: CCITT CCITT-Gruppe: 4 Graustufen glätten: Nein Text und Vektorgrafiken komprimieren: Ja SCHRIFTEN ---------------------------------------- Alle Schriften einbetten: Ja Untergruppen aller eingebetteten Schriften: Nein Wenn Einbetten fehlschlägt: Warnen und weiter Einbetten: Immer einbetten: [ ] Nie einbetten: [ /Symbol /ZapfDingbats /Courier-BoldOblique /Helvetica-BoldOblique /Courier /Helvetica-Bold /Times-Bold /Courier-Bold /Helvetica /Times-BoldItalic /Times-Roman /Times-Italic /Helvetica-Oblique /Courier-Oblique ] FARBE(N) ---------------------------------------- Farbmanagement: Farbumrechnungsmethode: Farbe nicht ändern Methode: Standard Geräteabhängige Daten: Einstellungen für Überdrucken beibehalten: Nein Unterfarbreduktion und Schwarzaufbau beibehalten: Ja Transferfunktionen: Beibehalten Rastereinstellungen beibehalten: Nein ERWEITERT ---------------------------------------- Optionen: Prolog/Epilog verwenden: Nein PostScript-Datei darf Einstellungen überschreiben: Nein Level 2 copypage-Semantik beibehalten: Ja Portable Job Ticket in PDF-Datei speichern: Nein Illustrator-Überdruckmodus: Ja Farbverläufe zu weichen Nuancen konvertieren: Ja ASCII-Format: Nein Document Structuring Conventions (DSC): DSC-Kommentare verarbeiten: Ja DSC-Warnungen protokollieren: Nein Für EPS-Dateien Seitengröße ändern und Grafiken zentrieren: Ja EPS-Info von DSC beibehalten: Ja OPI-Kommentare beibehalten: Nein Dokumentinfo von DSC beibehalten: Ja ANDERE ---------------------------------------- Distiller-Kern Version: 5000 ZIP-Komprimierung verwenden: Ja Optimierungen deaktivieren: Nein Bildspeicher: 524288 Byte Farbbilder glätten: Nein Graustufenbilder glätten: Nein Bilder (< 257 Farben) in indizierten Farbraum konvertieren: Ja sRGB ICC-Profil: sRGB IEC61966-2.1 ENDE DES REPORTS ---------------------------------------- IMPRESSED GmbH Bahrenfelder Chaussee 49 22761 Hamburg, Germany Tel. +49 40 897189-0 Fax +49 40 897189-71 Email: [email protected] Web: www.impressed.de
Today’s needs to perform Many different assaysWith Multiple labels
Are now met by One readerWith No compromise in terms of sensitivity.
The Mithras LB 940 operates with dedicated optical paths to ensure thebest available performance for each technology. In consequence, theimplemented detection technologies can be read with the performanceof dedicated instruments. Those consist of luminescence, fluorescence,absorbance and in addition the homogeneous methods BRET, FRET,AlphaScreen™ as well as Fluorescence Polarisation.
Ancient legend ...... says that on December 25th, Mithras was conceived in the
shade of a sacred tree beside a sacred stream, holding a knifeand a torch. A raven from the sun god Sol then gave him a
message that told him to slay the mysterious white bull.Mithras obeyed and tracked down the bull, accompanied by two minor deities, Cautes and Cautophes. When he finally
met up with the mystic bull, he did slay it, using the knife hewas born with. The white bull then became the moon and
Mithras’ vicorious cape became the sky. Supposedly, this evolved into the alteration of night and day. The legend endwith Mithras’ climbing into Sol’s golden chariot and flashing