Miniaturized Silicon Nanowire System for the In Vivo Diagnosis of Disease R. Myers, L. Rivera, Y. He, Z. Li and J. Villalba.

Miniaturized Silicon Nanowire System for the In Vivo Diagnosis of DiseaseR. Myers, L. Rivera, Y. He, Z. Li and J. Villalba

Technical Need46% of all cancer patients are diagnosed when cancer has reached an advanced stage

Current cancer diagnostic techniques are invasive and expensive

Similar Available Technology: Miriam by Miroculus

In vitro detection of disease through blood samples

Drawbacks: long diagnostic time, high price ($500 per device) and size

Technical ApproachTask 1:

Biomarker Screening

Task 2: Nanowire

Distribution

Task 3: Miniaturizati

on

Task 4: In Vitro Testing

Risk Mitigation

If Benchmark Biomarker fails

If Miniaturizatio

n fails

Task 1: Biomarker ScreeningSelect benchmark biomarker based on:

Sensitivity

Specificity

Size of biomarker-aptamer-cross-linker complex

FDA approved biomarkers from Polanski M., Anderson Leigh N., “A List of Candidate Cancer Biomarkers for Targeted Proteomics”, Biomarker Insights, Volume 1, Pages 1-48 (2007)

Task 2: Nanowire DistributionManufacture systems

by varying manufacturing conditions for CVD and thermal evaporation

Test for sensitivity and specificity

Goal: Determine optimal surface area to nanowire distribution ratio

Aptamer-Biomarker binding by lock-and-key mechanism from De Vico L. et al, “Quantifying signal changes in nano-wire based biosensors”, Nanoscale, Volume 3, Pages 706-717 (2011)

Task 3: Miniaturization

Determine safe implant size range

Scale down current systems based on optimal surface area to nanowire distribution ratio

Re-evaluate project based on results Silicon Nanowire between source and drain from Hsu S., Tsai C.,

Hsu W., Lu F., He J., Cheng K., Hsieh S., Wang H., Sun Y. and Tu L., “Fabrication of Silicon Nanowires Field Effect Transistors for Biosensor Applications”, Bioengineering Conference (NEBEC) 2012 38th Annual Northeast, Pages 5-6 (2012)

Task 4: In Vitro Testing Test sensitivity to ensure proper functionality of miniaturized system

Ensure that miniaturized system will:

Be resistant to clogging

Allow filtration by biomarker size

Ensure laminar flow

Maintain mechanical and conductive integrity

Microfluidic channel for silicon nanowire biosensor from Leydent M.T., Schuman C., Sharf T., Kevek J., Remcho V.T. and Minot E.D., “Fabrication and Characterization of Carbon Nanotube Field-Effect Transistor Biosensors”, Organic Semiconductors in Sensors and Bioelectronics III, Conference Volume 7779 (2010)

Project Timeline

Project Cost and Deliverables

Research plan and outcomes will be presented and updated at the end of each task

Will provide a working prototype of a miniaturized silicon nanowire biosensor to monitor biomarker levels in blood plasma

Prototype will be ready for integration into applications for In Vivo testing Project cost distribution. Total estimated cost for 4-year

research project is $1,295,682

Social and Economic Impact

Will allow for focus of resources on treatment instead of diagnosis

Early diagnosis system save thousands of lives and up to $16 billion in healthcare costs within the first five years

Opportunities for project growth by partnerships with medical research teams and insurance providers

There is no time to waste!Early diagnostic procedures save lives

and save money!