min 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 mAU 0 25 50 75 100 125 150 175 mAU min 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 0 25 50 75 100 125 150 175 min 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 mAU 0 25 50 75 100 125 150 175 3.5 um 5 um 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 4-Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid LC Conditions Column: SB-C18, 4.6 x 30 mm Detector: 254 nm Injector: 1 ul, Mobile Phase: 1% Formic Acid Aceton (82:18 Flow: 2.0 ml/min 1.8 um Aumento della Risoluzione in funzione del Particle Size
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Min 00.250.50.7511.251.51.752 mAU 0 25 50 75 100 125 150 175 mAU min 00.250.50.7511.251.51.752 0 25 50 75 100 125 150 175 min 00.250.50.7511.251.51.752.
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higher speed – same resolution – higher sensitivity/precision
2 ml/min – 90bar
3 ml/min – 133bar
4 ml/min – 181bar
2x faster than 2ml/min without loss in resolution!
Ultra-fast analysis in 26 sec4 peak width ~ 0.6 sec
< 50 sec cycle time
7 analgesics separated in 21 secfor peaks 1,2,3Total time Injection-to-Injection
HPLC, 40°CSB-C184.6 x 150mm, 5µm1.20ml/min, 40°CAnalysis Time = 11min
min0 2 4 6 8 10
Rapid Resolution HT Columns – Up to 20X FasterRapid Resolution HT Columns – Up to 20X Faster
RRHT SB-C182.1mm x 50mm 1.8µm1.00ml/min, 40°CAnalysis Time= 1.1min
RRLC, 40°C 10x faster
min0.2 0.4 0.6 0.8 10
0.2 0.60 0.4 min
RRHT SB-C182.1mm x 50mm 1.8µm2.40ml/min, 95°CAnalysis Time: 0.4min
RRLC, 95°C
27x faster >20X faster>20X faster
Up to 20x faster than conventional HPLC • With same or better performance (resolution, precision, sensitivity, carry over)• Compliant with strictest regulatory performance requirements