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Mild in vitro trauma induces rapid Glur2 endocytosis, robustly augments calcium permeability and enhances
susceptibility to secondary excitotoxic insult in cultured Purkinje cells
by Joshua D. Bell, Jinglu Ai, Yonghong Chen, and Andrew J. Baker
Validation of Purkinje cell culture system via immunocytochemical labelling using glial fibrillary acidic protein (GFAP) (A), calbindin D-28K (B), MAP2 (C,D) as markers for astrocytes, Purkinje
Immunocytochemical labelling of control and injured neurons for GluR2 (A & D respectively), synaptophysin (B & E respectively), and the overlay of the two (C and F respectively).
(A) Combination therapy of PKCα inhibition and blockade of calcium permeable GluR1/3 containing AMPA receptors confers profound neuroprotection against delayed cell death caused