Microscopy fits needs of Microscopy fits needs of researcher, type of researcher, type of specimen being viewed specimen being viewed Brightfield Brightfield Darkfield Darkfield DIC/Nomarski DIC/Nomarski Ultrastructural Ultrastructural Dimensional microscopy (SEM and Dimensional microscopy (SEM and Confocal) Confocal) Simple magnification Simple magnification
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Microscopy fits needs of researcher, type of specimen being viewed Brightfield Brightfield Darkfield Darkfield DIC/Nomarski DIC/Nomarski Ultrastructural.
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Microscopy fits needs of Microscopy fits needs of researcher, type of researcher, type of
specimen being viewedspecimen being viewed
BrightfieldBrightfield DarkfieldDarkfield DIC/NomarskiDIC/Nomarski UltrastructuralUltrastructural Dimensional microscopy (SEM and Dimensional microscopy (SEM and
Resolution Depends Upon Resolution Depends Upon Magnification, Type of Scope, Magnification, Type of Scope, Specimen Fixation, Thickness, Specimen Fixation, Thickness,
StainingStaining Staining types – GeneralStaining types – General
Basic stains (Methyl green, methylene blue)Basic stains (Methyl green, methylene blue) Mordants (Hematoxylin, a generally basic Mordants (Hematoxylin, a generally basic
dye, is a mixture of hematoxylin and mordant dye, is a mixture of hematoxylin and mordant (link b/w dye and tissue) generally used in (link b/w dye and tissue) generally used in connection with acidic dyes to provide connection with acidic dyes to provide contrast and staining of cellular componentscontrast and staining of cellular components
Specific tissue component stains (eg., PAS Specific tissue component stains (eg., PAS for carbohydrates)for carbohydrates)
AutoradiographyAutoradiography Incorporation of label into DNAIncorporation of label into DNA
Injection of labelled tracer into animal or cellInjection of labelled tracer into animal or cell Removal of tissue, fixation, sectioningRemoval of tissue, fixation, sectioning Exposure of section to photographic emulsionExposure of section to photographic emulsion Emission of radioactive tracer reacts with Emission of radioactive tracer reacts with
emulsionemulsion Emulsion is developed, silver grains denote Emulsion is developed, silver grains denote
label locationlabel location Counterstain and viewCounterstain and view
Areas of label indicative of cellular Areas of label indicative of cellular process (eg., making DNA, protein) being process (eg., making DNA, protein) being examinedexamined