Microscope Lab Do Now: Pick up lab and preread the whole lab, answer pre-lab questions when finished reading. Not before, after!

Microscope LabMicroscope Lab

Do Now: Pick up lab and preread the whole lab, answer pre-lab questions

when finished reading. Not before, after!

Do Now: Pick up lab and preread the whole lab, answer pre-lab questions

when finished reading. Not before, after!

Set up your Cornell Notes As Follows (skip lines……):Set up your Cornell Notes

As Follows (skip lines……): I. Safety II. Magnification III. Preparing Wet Mount IV. Focusing V. Electron Microscopes Define: resolving power, depth of

view

I. Safety II. Magnification III. Preparing Wet Mount IV. Focusing V. Electron Microscopes Define: resolving power, depth of

view

Compound Light Microscope

Compound Light Microscope

Always carry with 2 hands Arm and base

Place away from edge of table Always start with

stage all the way down Light off Lowest power

Always return the microscope to this position when finished

Always carry with 2 hands Arm and base

Place away from edge of table Always start with

stage all the way down Light off Lowest power

Always return the microscope to this position when finished

• 1• 2• 3• 4• 5• 6• 7• 8• 9• 10• 11• 12• 13• 14• 15

• 1• 2• 3• 4• 5• 6• 7• 8• 9• 10• 11• 12• 13• 14• 15

Eyepiece Course

FocusFine FocusScanning

ObjArm Power Body TubeNose piece High

PowerLow PowerClips Stage Diaphragm Light Base

Eyepiece Course

FocusFine FocusScanning

ObjArm Power Body TubeNose piece High

PowerLow PowerClips Stage Diaphragm Light Base

MagnificationMagnification• Eyepiece

– What you look through– May have pointer inside– 10x

• Rotating nosepiece has 3 objectives– Scanning (short and red) 4x– Low (medium and yellow) 10x– High (long and blue) 40 x

• Eyepiece– What you look through– May have pointer inside– 10x

• Rotating nosepiece has 3 objectives– Scanning (short and red) 4x– Low (medium and yellow) 10x– High (long and blue) 40 x

Total Magnification =Eyepiece X objectiveTotal Magnification =Eyepiece X objective

• Eyepiece• 10x

• Eyepiece• 10x

• Scanning 4x = 40x total• Low 10x = 100x total• High 40x = 400x total

• Scanning 4x = 40x total• Low 10x = 100x total• High 40x = 400x total

Controlling lightControlling light

• First plug in and turn on the light.

• Under the stage is a rotating disc that turns and clicks

• It may have numbers on it• Turning the diaphragm (disc)

controls the amount of light

• First plug in and turn on the light.

• Under the stage is a rotating disc that turns and clicks

• It may have numbers on it• Turning the diaphragm (disc)

controls the amount of light

Slide #1• Prepared slide of crossed threads

• Place slide on stage and secure• Use scanning objective and try to center the

crossed threads then focus– Use course focus (large knob) first– Fine focus (small knob) second– Which way does the slide move if you push it left? Push

right?– What affect does changing the diaphragm have on the

image?

• Change to low power– Notice any change?– Draw

• Change to high power– USE ONLY FINE FOCUS!– Course focus can press into slide and crack

the glass, squish specimen, and damage the objective.

– It may take time to focus correctly - practice– How does the view of the image change?– Draw

NO SQUISH THE SLIDE!!!!!!

The Wonderful Letter e• Preparing a wet-mount

– Cut out a lower case letter e– Place on slide– Carefully add water drop– Try to keep the e looking like this… e– Carefully place cover slip on letter

• Place slide on microscope and secure

• Start with scanning objective - center and focus. See any thing unusual?

• Change to low power and draw

• Change to high power and draw

Depth of View

• Notice that as you increase magnification you see more detail of less of the image.

• You also see layer of the image that you can focus through

• More of less

Resolving Power• A measure of the clarity of an image.

Closure• Complete post lab questions

• Turn in lab

Electron MicroscopeElectron Microscope

• Scanning or SEM• Uses electrons bounced

off the surface of the specimen

• Transmission or TEM• Electrons through the

specimen

• Live specimens cannot be used

• Scanning or SEM• Uses electrons bounced

off the surface of the specimen

• Transmission or TEM• Electrons through the

specimen

• Live specimens cannot be used

Electron Microscope (2 kinds)

Electron Microscope (2 kinds)

• *• *

Transmission Electron Microscope

Transmission Electron Microscope

• TEM-• Electrons go • Through the• Specimen• (non living • Specimens• Only)

• TEM-• Electrons go • Through the• Specimen• (non living • Specimens• Only)

Scanning Electron Microscope

Scanning Electron Microscope

• SEM –• Uses electrons• Bounced off the• Surface of the • Specimen• (non living• Specimens only)

• SEM –• Uses electrons• Bounced off the• Surface of the • Specimen• (non living• Specimens only)