Microscope Introduction

Introduction to the Microscope

CarePartsFocusing

• Magnification – making the object bigger

• Resolution – how clearly you can see the object

• http://brunelleschi.imss.fi.it/esplora/microscopio/dswmedia/simula/esimula1.html

“Light” because we use light to see the images

“Compound” because it uses two lenses to view a specimen.

Light vs. Electron

Light vs. Electron

Light vs. Electron

SEM vs. TEM

Eyepiece orOcular Lens

Body Tube

Revolving NosepieceArm

Objective Lenses

StageStage Clips

Coarse Focus

Fine Focus

Base

Diaphragm

Light

• Always carry with 2 hands - by the arm and the base.

• Only use lens paper for cleaning and don’t touch the lenses to anything (including your fingers.)

• Always store covered• Don’t let cords hang where the

microscope could be pulled off the table.

• Ocular lens X Objective lens

• If the ocular lens is 10X and the objective lens is 5X, the total magnification would be:

• Place the Slide on the Microscope• Use stage clips only when you are sure

you don’t want to move the slide around.

• Click Nosepiece to the lowest (shortest) setting

• Start with the objective closest to the specimen.

• Use the Coarse Focus to move objective AWAY from specimen.

• Have focused on low power.

• Click the nosepiece to the next biggest objective.

• Do NOT use the Coarse Focusing Knob

Use the fine focus knob to bring the slide

• Do NOT force any knobs. They should all move easily.

• Do NOT turn the fine focus more than a full turn. Start over on low power if you have to.

Elodea

Onion Cells

Cheek Cells