Microbiology and Parasitology - University of Benghazipharm.uob.edu.ly/assets/uploads/pagedownloads/2d58… · · 2017-05-04Course Specification Microbiology and Parasitology, 2012-2013

Course Specification

Microbiology and

Parasitology, 2012-2013

Faculty of pharmacy

Benghazi University

1

Microbiology and Parasitology

Faculty of Pharmacy - Benghazi University


Program on which the course is given: Bachelor Degree of Pharmaceutical

Sciences

Major or Minor element of programs: Single major

Department offering the programs: Interdisciplinary-

Department offering the course: Microbiology and Parasitology

Academic year / level: Third year

Date of Specification approval: 10/2012

A. Basic Information

Title: Microbiology and Parasitology

Code: P307

Course language: English

Lectures: 4 hrs/week

Practical: 4 hrs/week

Total: 8 hrs/week

Credit Hours: 6 hours

B. Professional Information

1. Overall Objectives of Course:

After completing the Program of Study in Microbiology and Parasitology, students should

be able to: Demonstrate an understanding of core concepts of microbiology and

Parasitology, including history of microbiology and parasitology and the pioneers in this

fields; bacterial and parasite cell structure; function; and metabolism.


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Apply the basic principles of kinetic of bacterial growth and quantitative reasoning to solve

problems in microbiology.

Display proficiency in basic microbiological and parasitological skills.

Demonstrate an understanding of hypothesis testing and experimental design in

Sterilization process.

Understand the basic, structure and mechanism of action of antibiotics.

Understand the mechanism of resistance to antibiotics.

Understanding the different non antibiotic antimicrobial agents and their mechanism of

action.

Understand all scientific disciplines of microscopes and parasites.

Be able to identify different microorganisms and recognize different extracellular products

on the microbial cells.

The student should be familiar with different categories of microorganisms including

bacteria, viruses, fungi & parasites regarding classification, structure, growth

characteristics & replication of each microbial category.

Understand the different infectious diseases, their diagnosis clinical symptoms or their

laboratory diagnosis as well as, their portal of entry to human, and the method of

prevention and control.

The student should also have a basic background about microbial genetics, immune

system & immune response.

Understand the scientific concept, diagnosis and control of parasitic infection.

Communicate the fundamental concepts of Microbiology and Parasitology, both in written

and in oral format.

Analyze, interpret and evaluate a range of scientific literature in microbiology.

2. Intended Learning Outcomes of Course (ILOs):

A. Knowledge and Understanding:

By the end of the course, students should be able to:

a1. Know the main information about microbial characteristics, bacterial metabolism and

bacterial growth.

a2. Define basics of microbial genetics.

a3. Discover principles and theories of sterilization process.

a4. Identify the different types of antibiotics, antiseptics and disinfectants and their

application in addition of different methods which used in the evaluation of their

effects.

a5. Know biotechnological concepts with mention of some methods used for producing

bioactive compounds in industry scale.

a6. Define basics of immunology and serological tests which use in detection of different

infectious diseases.


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a7. Know main information about different microbes causing infective diseases (bacterial,

viral & fungal), methods of diagnosis, treatment and prevention.

a.8 Name parasites causing diseases to man.

a.9 Mention the geographical distribution of each parasite.

a.10 Localize the organs inhabited by the parasite.

a.11 Describe the morphological features of parasite.

a.12 Identify the mode of infection with parasite.

a.13 Describe the life cycle of the parasite.

a.14 Describe the clinical presentations of the parasite.

a.15 Enumerate the diagnostic techniques.

a.16 Write the treatment of each parasitic disease.

a.17 Name the preventive measures to avoid the infection by this parasite in relation to the

environmental factors.

a.18 Identify how to use the microscope.

B. Intellectual Skills:

The students should be able to:

b1. Evaluate certain techniques for isolation and identification of microorganisms and their

classification.

b2. Distinguish the mechanism of action and uses of the different methods of sterilization.

b3. Distinguish the effectiveness of different antibiotics and disinfectants via certain methods.

b4. Interpret different techniques which determine considerable microbiological measures.

b5. Analyze cases of study and suggest the infectious disease and the possible causative

agent.

b5. Interpret different diagnostic tests which aid in the identification of causative agents of

several diseases.

b.6. Recognize the data obtained from the course

b.7. Interpret the laboratory data

b.8. Differentiate and compare the similar stages of different parasites.

b.9 Interpret the differential parasitic causes of a disease.

C. Professional and Practical Skills


c1. Perform different techniques of bacterial staining.


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c2. Isolate and identify pure colonies of microorganisms from different types of specimens.

c3. Perform different methods to sterilize articles.

c4. Perform different practical methods to evaluate the effectiveness of different types of

antibiotics and disinfectants.

c4. Perform different serological techniques.

c5. Identify different microorganisms regarding to their morphological characteristics (shape,

size and color of the colony) and biochemical reactions on different types of media.

c.6. Perform some of the essential laboratory techniques.

c.7. Examine the slide to identify different parasitic stage.

c.8 Practice different laboratory techniques and how to perform some of them.

c.9- Examine and identify grossly parasites and their stages and parasitic lesions in different organs.

c.10- Examine and identify the microscopic morphology of parasites and their adult, larval stages

egg and cercaria.

c.11- Examine, identify, differentiate and classify arthropods of medical importance.

D. General and Transferable Skills


d1. Retrieve and evaluate information collected during practical sections.

d2. Communicate clearly by verbal means through group discussions.

d3. Implement writing and presentation skills through creation of a research proposal.

d.4 Retrieve recent data from web sites.

d.5 Work productively in a team.

d.6 Obtain, interpret and synthesise information from background reading and use it to

effectively supplement lecture notes.

d.7 Appreciate the team work.

d.8 Show respect to paramedical personnel and laboratory workers.

d.9 Respect the patient confidentiality.


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3. Course Contents:

a. Theoretical Part:

Topic

No.

of

hours

Contents

General Microbiology:

10

History.

General properties of microbial types, Classification,

morphology and staining.

Bacterial metabolism.

Bacterial growth and factors affect microbial death.

Viruses; morphology & virus like particles.

Microbial genetics:

6

Central dogma theory.

DNA & RNA (replication, transcription, translation).

Regulation of protein synthesis.

Bacterial genetic variation.

Mutation.

Recombination, transformation, transduction,

conjugation and gene cloning.

Sterilization 8

Introduction.:

Sterilization indicators.

Sterility testing.

Microbial contamination of pharmaceutical products.

Aseptic area.

The microbiological quality of pharmaceuticals.

Antimicrobial

chemotherapeutic

agents:

5

History.

Classification of antibiotics.

Mode of action of different antibiotics.

Examples of antibiotics and antiviral agents.

Non antibiotic

antimicrobial agents:

2

Types of antimicrobial agents, mode of action and

resistance to antimicrobial agents.

Evaluation of antimicrobials and other agents.

antisepsis, disinfection and preservation.

Biotechnology

4

Introduction.

Biology of industrial micro-organisms.

Biology of industrial micro-organisms.

Biophysical and biochemical processes.

Introduction to tissue culture and genetic engineering

techniques.

Techniques for the improvement of the economically

important plants and animals and for the

development of micro-organisms to act on the

environment.

Manipulation of living organisms, especially at the

molecular genetic level, to produce new products,

such as hormones, vaccines or monoclonal

antibodies.

Production of pharmaceuticals by microorganisms.


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Topic

No.

of

hours

Contents

Gene therapy.

Immunology

6

Non-specific & specific immunity.

Cells involved in the immune response

Types of immunoglobulin & mechanism of antibody

production.

Hypersensitivity reactions.

Serological tests.

Medical Bacteriology 20

Gram positive cocci.

Gram negative cocci

Gram positive rods.

Gram negative rods.

Chlamydiae and rickettsiaceae.

Mycology.

Mycology 4

Study the morphological and characters of different

fungi; their nature; chemical composition of cell wall;

fungal reproduction as well as classification of fungi.

Different important mycotic diseases: including

symptoms, diagnosis and treatment.

Virology

10 Introduction.

DNA viruses.

RNA viruses

3) Course contents of Parasitology:

Subject Lectures

(hrs)

Tutorial / Small

group discussion

(hrs)

Practical

(hrs)

Total

(hrs)

% of Total

1- Introduction to

parasitology

1 1

2-Helminthology,

Trematodes and

Cestodes

10 10 10 30

3- Introduction to

Nematodes, intestinal

and tissue nematodes

6 5 5 16

4- Protozology, 3 5 5 13


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Intestinal, Urogenital,

blood and Tissue

protozoa

5- Immunology and

molecular parasitology

4 3 3 10

6- Entomology 1 2 2 5

total 25 25 25 100

b. Practical Part:

Topics No. of

hours Pharmaceutical Microbiology:

Safety rules in the laboratory & microscope.

1 Simple staining

Gram staining

1

Practice on Gram stain mixture.

Acid- Fast stain (Zeil Nelsen stain) & spore stain.

1

Motility

Pouring agar plates, isolation in pure culture, & distribution of microorganisms

viable count

2

Culture sensitivity to different antibiotics (disk diffusion method).

Determination of M.I.C by cup plate technique.

2

Determination of M.I.C by serial dilution method. 2 Antibiotic assay. 2 Effect of different antiseptics by cup plate agar diffusion method. 1 Extraction and Purification of DNA.

Amplification of DNA by polymerase chain reaction

2

DNA and separation by agarose gel electrophoresis 2 Polyachrylamide gel electrophoresis

ELISA

2

Immunology.

Antigen antibody reaction

Agglutination test.

Blood grouping.

Pregnancy test.

Widal test.

4


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Ouchterlony test.

ASO titre

Complement fixation test.

Medical Bacteriology

Gram positive cocci.

Gram negative cocci.

Gram positive Spore forming rods.

Gram positive non-spore forming rods.

Gram negative rods.

2

2

2

6 Revision 2 Parasitology.

Class Trematoda.

Class Cestoda.

Class Nematoda.

Arthropods.

III-A) THEORETICAL TOPICS:

1. Introduction to parasitology

2. Helminthology

a. Trematodes

b. Cestodes

c. Nematodes

3. Protozoology

4. Immunology and molecular parasitology

5. Entomology

III-B) PRACTICAL TOPICS:

A) Identification of different parasitic stages

1- Examination of adults, eggs, larva of Fasciola.

2- Examination of adult male and female, eggs, cercaria of Schistsoma.

3- Examination of adult, egg, of Paragonimus.

4- Examination of scolex, mature segment and egg of D. latum. scolex, mature and gravid segment

of Taenia. Cysticercous cellulosa . C. bovis

5- Examination of scolex, mature, gravid segment, eggs of H. nana, H. diminuta, D.

caninum and cysticercoids.


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6- Examination of adult Ecchinococcus , hydatid cyst , hydatid sand.

7- Examination of anterior, posterior ends of adult Ascaris and eggs.

8- Examination of adult male, female Enterobius and Trichuris and their eggs.

9- Examination of buccal capsule, bursa, egg and filariform larva of Ancylostoma, Necator buccal

capsule.

10- Examination of T. spiralis encysted larva.

11- Examination of W. bancrofti microfilaria.

12- Examination of Onchocerca in subcutaneous nodule.

13- Examination of E . histolytica and E .coli trophozoites , cysts.

14- Examination of Giardia trophozoite, cyst. Trichomonas trophozoite

15- Examination of Leishmania bodies. Leptomonad form

16- Examination of African Trypanosomiasis.

17- Examination of Plasmodium ring stage, gametocytes.

18- Examination of Toxoplasma trophozoite

19- Examination of B. coli trophozoite and cyst. Cryptosporidium oocysts

20- Examination of mosquitoes, Anopheles, Culex, Aedes, mouth parts, male, female, wings, larva,

pupa.

21- Fleas, adult male and female, eggs, wing, larva. Lice adult male, female, eggs, nymph, Phthirus

(male, female)

22- Cimex (male, female, nymph, egg). Hard tick (adult male, female and larva). Soft tick adult and

larva

23- Sarcoptes (male, female).

B) Case Study.

C) Demonstration of different parasitological techniques.

a. Direct stool examination

b. Concentration techniques

c. Blood smears

d. Lymph smear

Biochemistry Course Specification

2012-2013

Faculty of pharmacy

Benghazi University

4. Matrix of achievement of course ILOs in course contents:

Topic

Knowledge and Understanding Intellectual Skills Practical and Professional Skills General and transferable

Skills

A

1

A

2

A

3

A

4

A

5

A

6

A

7

A

8

A

9

A

10

A

11

A

12

A

13

A

14

A

15

B

1

B

2

B

3

B

4

B

5

C

1

C

2

C

3

C

4

C

5

C

6

C

7

C

8

C

9

C

10

D

1

D

2

D

3

D

4

D

5

D

6

Microbial

genetics:

X X X X X X X

Sterilization X X X X X X X X X X X X

Antimicrobial

chemotherapeutic

agents:

X X X X X X X

Non antibiotic

antimicrobial

agents:

X X X X X X X X X X X X

Biotechnology

X X X X X X X

Immunology

X X X X X X X X X X X X

Medical

Bacteriology X X X X X X X X X X

Mycology X X X X X X X X X X

Virology

X X X X X X X X X X

Parasitology X X X X X X X X


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5. Teaching and Learning Methods

Lectures (board, data show).

Practical sessions (PPP and some practical's experiments).

Self learning in the form of student research and presentation.

Office hours

Small group discussions to answer the questions of the students.

6. Student Assessment Methods

a. Attendance Criteria:

The minimum acceptable practical attendance is 75%; students who fail to

attend that percentage of activities will lose 30 marks out of the practical

marks.

b. Assessment Tools:

Tool Purpose

Student research and

presentation Assessment of general and transferable skills

Written examination

Assessment of knowledge and understanding

and intellectual skills.

Oral examination

Assessment of knowledge and

understanding, intellectual, general and

transferable skills.

Practical examination

Assessment of intellectual and transferable

skills.

c. Time Schedule of assessment:

Practical:

Evaluation of students' performance and activity during labs

Student presentation evaluation

Quizzes:


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1st semester: Two equal exams: one held in December and the other in

January for all students.

Those who don't attend the examination(s) for acceptable reason(s);

their marks will be raised as a proportion from the final written

examination score.

Final examination:

At the end of the academic year (May) for all students in the form of

oral and written exams

The exam is re-held in November for those who fail to pass the final

exam or postpone it

d. Grading system:

Examination Marks allocated

Practical

Performance during Lab 50

Student presentation 10

Quizze

December 2012 30

January 2013 30

Final examination

Written 135

Oral 45

Total 300

The minimum passing score is 180 marks provided at least 36 marks are

obtained in the final written examination.


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Passing grades are:

Grade Percentage

Excellent ≥ 85%

Very good 75 - 85%

Good 65 - 75%

Pass 60 - 65%.

e. Examination description:

Examination Description Marks

Practical

Regular Evaluation of

Performance during Lab

Student presentation

60

Quizzes One hour MCQ exam (70%) +

short essy (30%). 60

Final Written

examination

A 3-hour written paper composed

of MCQ 135

Final oral

examination One oral examination station 45

Total 300

7. Learning and reference materials:

a. Basic materials:

Overhead projections and computer presentations used during

teaching in classes.

Notes on the laboratory practical work and soft copy .

b. Suggested materials:

Warren Levinson& Ernest Jawetz. Medical Microbiology and

Immunology Fourth edition. Prentice –Hall International, Inc, 1996.

J.G Collee, J.P. Duguid, A.G. Fraser and B.P.Marmion. Practical Medical

Microbiology. Edinburgh London Melbourne and New York, 1989.


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c. Periodicals, Web sites, … etc

www.asm.org

www.cdc.org

Essential books (text books):

Medical parasitology,

Foundation of Parasitology

Medical Diagnostic Parasitology,

Web sites: www.dpd.cdc.org, www.abebooks.com

8. Facilities required for teaching and learning

Study halls, data show, books, laboratories, instruments and microscopes.

Course coordinator:

Dr./ Ahmed Abdel-Rady

Head of Department:

Dr./ Ahmed Abdel-Rady

Date: 10/2012

http://www.asm.org/

http://www.cdc.org/

http://www.dpd.cdc.org/

Microbiology and Parasitology - University of Benghazipharm.uob.edu.ly/assets/uploads/pagedownloads/2d58… · · 2017-05-04Course Specification Microbiology and Parasitology, 2012-2013

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